Assessment of the Target Engagement and d-Serine Biomarker Profiles of the d-Amino Acid Oxidase Inhibitors Sodium Benzoate and PGM030756

Neurochemical Research - Irregular N-methyl-d-aspartate receptor (NMDAR) function is one of the main hypotheses employed to facilitate understanding of the underlying disease state of...     

Thrombopoietin: a pan-hematopoietic cytokine.

The recent discovery of thrombopoietin has enhanced our understanding of both hematopoiesis and platelet production. Thrombopoietin supports hematopoietic stem cell survival and expansion as well as promoting all aspects of megakaryocyte development. The hormone displays many structural similarities to other members of the hematopoietic cytokine family and some notable differences, and regulation of its expression requires both receptor-mediated removal and other mechanisms. Thrombopoietin induces receptor dimerization and tyrosine phosphorylation, and a series of signaling events including activation of JAK/STAT, Shc/Ras/MAPK and PI3K/Akt; these pathways overlap with those induced by other cytokines, but the differences that lead to the unique biological effects of the hormone are gradually being uncovered. Our growing appreciation of how cytokine signaling pathways are translated into megakaryocyte development is discussed.     

New times for biology: nerve cultures and the advent of cellular life in vitro

This article is about the beginnings of tissue culture—the culture of living, reproducing cells of complex organisms outside the body. It argues that Ross Harrison’s experiments in nerve culture between 1907 and 1910 should be viewed as part of a larger shift in early twentieth-century laboratory practice from in vivo to in vitro experimentation. Via a focus on the temporality of experiment—contrasting the live object of Harrison’s investigation with the static object of histological representations—this article details the production of a new and surprising form of life, cellular life in vitro. Tissue culture, developed from Harrison’s experiments, was greeted with great surprise and disbelief, despite Harrison’s protestations that he had merely juxtaposed extant techniques. An analysis of these initial reactions to tissue culture illuminates the extent to which cells living visibly outside of the body in glass broke with in vivo practices and assumptions of the hiddenness and interiority of certain processes of growth and change.     

Minimal moisture content for growth and aflatoxin production by Aspergillus parasiticus in mixed feeds

Minimal moisture content for growth and aflatoxin production by Aspergillus parasiticus in mixed feeds was studied. Minimal moisture content for growth is 16.51%+/–0.45. Very low amounts of aflatoxins are accumulated at days 1 or 2 after the growth started when the initial moisture content of the mixed feed was 17% or lower; on the other hand, significant amounts of aflatoxin are detected when it was 18% or higher.