全文获取类型
收费全文 | 3625篇 |
免费 | 364篇 |
国内免费 | 1篇 |
出版年
2023年 | 36篇 |
2022年 | 105篇 |
2021年 | 172篇 |
2020年 | 77篇 |
2019年 | 92篇 |
2018年 | 95篇 |
2017年 | 83篇 |
2016年 | 143篇 |
2015年 | 218篇 |
2014年 | 215篇 |
2013年 | 224篇 |
2012年 | 290篇 |
2011年 | 251篇 |
2010年 | 160篇 |
2009年 | 144篇 |
2008年 | 198篇 |
2007年 | 167篇 |
2006年 | 163篇 |
2005年 | 151篇 |
2004年 | 138篇 |
2003年 | 106篇 |
2002年 | 113篇 |
2001年 | 63篇 |
2000年 | 75篇 |
1999年 | 51篇 |
1998年 | 37篇 |
1997年 | 21篇 |
1996年 | 20篇 |
1995年 | 17篇 |
1994年 | 26篇 |
1993年 | 13篇 |
1992年 | 33篇 |
1991年 | 33篇 |
1990年 | 25篇 |
1989年 | 26篇 |
1988年 | 24篇 |
1987年 | 20篇 |
1986年 | 16篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 6篇 |
1981年 | 8篇 |
1980年 | 9篇 |
1979年 | 8篇 |
1978年 | 9篇 |
1977年 | 9篇 |
1976年 | 8篇 |
1975年 | 6篇 |
1974年 | 6篇 |
1955年 | 4篇 |
排序方式: 共有3990条查询结果,搜索用时 15 毫秒
961.
Mitochondria form a highly dynamic reticular network in living cells, and undergo continuous fusion/fission events and changes in ultrastructural architecture. Although significant progress has been made in elucidating the molecular events underlying these processes, their relevance to normal cell function remains largely unexplored. Emerging evidence, however, suggests an important role for mitochondrial dynamics in cellular apoptosis. The mitochondria is at the core of the intrinsic apoptosis pathway, and provides a reservoir for protein factors that induce caspase activation and chromosome fragmentation. Additionally, mitochondria modulate Ca2+ homeostasis and are a source of various metabolites, including reactive oxygen species, that have the potential to function as second messengers in response to apoptotic stimuli. One of the mitochondrial factors required for activation of caspases in most intrinsic apoptotic pathways, cytochrome c, is largely sequestered within the intracristae compartment, and must migrate into the boundary intermembrane space in order to allow passage across the outer membrane to the cytosol. Recent evidence argues that inner mitochondrial membrane dynamics regulate this process. Here, we review the contribution of mitochondrial dynamics to the intrinsic apoptosis pathway, with emphasis on the inner membrane. 相似文献
962.
This study examined the effect of dietary alpha-tocopheryl acetate on lipid and cholesterol oxidation in retail packed chicken meat during refrigerated storage. Male broiler chicks were randomly assigned to five pens containing 30 chicks each, which was subjected to one of five dietary treatments (0, 50, 100, 200, or 400 IU of alpha-tocopheryl acetate/kg diet). Five different levels of alpha-tocopherol were supplied to the chicks from 3 to 6 weeks. After 42 d of feeding all the broilers were slaughtered, and the carcasses were packed in polyethylene bags individually, bags similar to those used in the retail trade, and stored for 12 d at 4 degrees C. Growth performance and fatty acid composition were not affected by the dietary alpha-tocopherol levels. The alpha-tocopherol content in breast and thigh muscles increased as the level of dietary alpha-tocopherol increased. The supplementation with 200 or 400 IU of alpha-tocopherol was more effective in reducing the level of lipid oxidation (P<0.05) and total cholesterol oxidation products (P<0.05). Therefore, an increase in the dietary alpha-tocopherol level from 200 to 400 IU/kg feed causes major improvements in the oxidative stability of chicken meat during refrigerated storage. 相似文献
963.
Hannah L. Stewart Sally J. Holbrook Russell J. Schmitt Andrew J. Brooks 《Coral reefs (Online)》2006,25(4):609-615
Stony corals are the foundation of coral reef ecosystems and form associations with other reef species. Many of these associations
may be ecologically important and play a role in maintaining the health and diversity of reef systems, rendering it critical
to understand the influence of symbiotic organisms in mediating responses to perturbation. This study demonstrates the importance
of an association with trapeziid crabs in reducing adverse effects of sediments deposited on corals. In a field experiment,
mortality rates of two species of branching corals were significantly lowered by the presence of crabs. All outplanted corals
with crabs survived whereas 45–80% of corals without crabs died within a month. For surviving corals that lacked crabs, growth
was slower and tissue bleaching and sediment load were higher. Laboratory experiments revealed that corals with crabs shed
substantially more of the sediments deposited on coral surfaces, but also that crabs were most effective at removing grain
sizes that were most damaging to coral tissues. The mechanism underlying this symbiotic relationship has not been recognized
previously, and its role in maintaining coral health is likely to become even more critical as reefs worldwide experience
increasing sedimentation. 相似文献
964.
965.
Translation inhibition reveals interaction of 2′-deoxy and 2′-O-methyl molecular beacons with mRNA targets in living cells 下载免费PDF全文
Understanding the interaction between oligonucleotide probes and RNA targets in living cells is important for biological and clinical studies of gene expression in vivo. Here, we demonstrate that starvation of cells and translation inhibition by blocking the mTOR or PI-3 kinase pathway could significantly reduce the fluorescence signal from 2′-deoxy molecular beacons (MBs) targeting K-ras and GAPDH mRNAs in living cells. However, the intensity and localization of fluorescence signal from MBs targeting nontranslated 28S rRNA remained the same in normal and translation-inhibited cells. We also found that, in targeting K-ras and GAPDH mRNAs, the signal level from MBs with 2′-O-methyl backbone did not change when translation was repressed. Taken together, our findings suggest that MBs with DNA backbone hybridize preferentially with mRNAs in their translational state in living cells, whereas those with 2′-O-methyl chemistry tend to hybridize to mRNA targets in both translational and nontranslational states. This work may thus provide a significant insight into probe design for detection of RNA molecules in living cells and RNA biology. 相似文献
966.
Takács S Bottomley H Andreller I Zaradnik T Schwarz J Bennett R Strong W Gries G 《Proceedings. Biological sciences / The Royal Society》2009,276(1657):649-655
Foraging animals use diverse cues to locate resources. Common foraging cues have visual, auditory, olfactory, tactile or gustatory characteristics. Here, we show a foraging herbivore using infrared (IR) radiation from living plants as a host-finding cue. We present data revealing that (i) conifer cones are warmer and emit more near-, mid- and long-range IR radiation than needles, (ii) cone-feeding western conifer seed bugs, Leptoglossus occidentalis (Hemiptera: Coreidae), possess IR receptive organs and orient towards experimental IR cues, and (iii) occlusion of the insects' IR receptors impairs IR perception. The conifers' cost of attracting cone-feeding insects may be offset by occasional mast seeding resulting in cone crops too large to be effectively exploited by herbivores. 相似文献
967.
968.
969.
970.
Alison L. Galvin Lu Kang Itoro Udofia Louise M. Jennings Hannah M.J. McEwen Zhongmin Jin John Fisher 《Journal of biomechanics》2009,42(12):1898-1902
Ultra high molecular weight polyethylene (PE) remains the primary bearing surface of choice in total knee replacements (TKR). Wear is controlled by levels of cross-shear motion and contact stress. The aim of this study was to compare the wear of fixed-bearing total knee replacements with curved and flat inserts and to test the hypothesis that the flat inserts which give higher contact stresses and smaller contact areas would lead to lower levels of surface wear. A low-conforming, high contact stress knee with a low-medium level of cross shear resulted in significantly lower wear rates in comparison to a standard cruciate sacrificing fixed-bearing knee. The low wear solution found in the knee simulator was supported by fundamental studies of wear as a function of pressure and cross shear in the pin on plate system. Current designs of fixed-bearing knees do not offer this low wear solution due to their medium cross shear, moderate conformity and medium contact stress. 相似文献