全文获取类型
收费全文 | 2971篇 |
免费 | 419篇 |
国内免费 | 2篇 |
专业分类
3392篇 |
出版年
2023年 | 37篇 |
2022年 | 100篇 |
2021年 | 168篇 |
2020年 | 68篇 |
2019年 | 88篇 |
2018年 | 91篇 |
2017年 | 73篇 |
2016年 | 119篇 |
2015年 | 195篇 |
2014年 | 175篇 |
2013年 | 193篇 |
2012年 | 249篇 |
2011年 | 216篇 |
2010年 | 129篇 |
2009年 | 112篇 |
2008年 | 154篇 |
2007年 | 117篇 |
2006年 | 131篇 |
2005年 | 103篇 |
2004年 | 99篇 |
2003年 | 75篇 |
2002年 | 79篇 |
2001年 | 40篇 |
2000年 | 40篇 |
1999年 | 20篇 |
1998年 | 20篇 |
1997年 | 19篇 |
1996年 | 21篇 |
1995年 | 13篇 |
1994年 | 24篇 |
1993年 | 23篇 |
1992年 | 26篇 |
1991年 | 22篇 |
1990年 | 32篇 |
1989年 | 24篇 |
1988年 | 18篇 |
1987年 | 13篇 |
1986年 | 18篇 |
1985年 | 15篇 |
1984年 | 17篇 |
1980年 | 12篇 |
1979年 | 13篇 |
1978年 | 10篇 |
1977年 | 11篇 |
1976年 | 9篇 |
1974年 | 11篇 |
1972年 | 9篇 |
1971年 | 12篇 |
1969年 | 9篇 |
1968年 | 9篇 |
排序方式: 共有3392条查询结果,搜索用时 15 毫秒
81.
82.
Sil H. J. van Lieshout Amanda Bretman Chris Newman Christina D. Buesching David W. Macdonald Hannah L. Dugdale 《Molecular ecology》2019,28(18):4152-4165
Individual variation in survival probability due to differential responses to early‐life environmental conditions is important in the evolution of life histories and senescence. A biomarker allowing quantification of such individual variation, and which links early‐life environmental conditions with survival by providing a measure of conditions experienced, is telomere length. Here, we examined telomere dynamics among 24 cohorts of European badgers (Meles meles). We found a complex cross‐sectional relationship between telomere length and age, with no apparent loss over the first 29 months, but with both decreases and increases in telomere length at older ages. Overall, we found low within‐individual consistency in telomere length across individual lifetimes. Importantly, we also observed increases in telomere length within individuals, which could not be explained by measurement error alone. We found no significant sex differences in telomere length, and provide evidence that early‐life telomere length predicts lifespan. However, while early‐life telomere length predicted survival to adulthood (≥1 year old), early‐life telomere length did not predict adult survival probability. Furthermore, adult telomere length did not predict survival to the subsequent year. These results show that the relationship between early‐life telomere length and lifespan was driven by conditions in early‐life, where early‐life telomere length varied strongly among cohorts. Our data provide evidence for associations between early‐life telomere length and individual life history, and highlight the dynamics of telomere length across individual lifetimes due to individuals experiencing different early‐life environments. 相似文献
83.
84.
85.
86.
87.
88.
Tzu-Wen Liu Young-Min Park Hannah D. Holscher Jaume Padilla Rebecca J. Scroggins Rebecca Welly Steven L. Britton Lauren G. Koch Victoria J. Vieira-Potter Kelly S. Swanson 《PloS one》2015,10(8)
The gut microbiota is considered a relevant factor in obesity and associated metabolic diseases, for which postmenopausal women are particularly at risk. Increasing physical activity has been recognized as an efficacious approach to prevent or treat obesity, yet the impact of physical activity on the microbiota remains under-investigated. We examined the impacts of voluntary exercise on host metabolism and gut microbiota in ovariectomized (OVX) high capacity (HCR) and low capacity running (LCR) rats. HCR and LCR rats (age = 27wk) were OVX and fed a high-fat diet (45% kcal fat) ad libitum and housed in cages equipped with (exercise, EX) or without (sedentary, SED) running wheels for 11wk (n = 7-8/group). We hypothesized that increased physical activity would hinder weight gain, increase metabolic health and shift the microbiota of LCR rats, resulting in populations more similar to that of HCR rats. Animals were compared for characteristic metabolic parameters including body composition, lipid profile and energy expenditure; whereas cecal digesta were collected for DNA extraction. 16S rRNA gene-based amplicon Illumina MiSeq sequencing was performed, followed by analysis using QIIME 1.8.0 to assess cecal microbiota. Voluntary exercise decreased body and fat mass, and normalized fasting NEFA concentrations of LCR rats, despite only running one-third the distance of HCR rats. Exercise, however, increased food intake, weight gain and fat mass of HCR rats. Exercise clustered the gut microbial community of LCR rats, which separated them from the other groups. Assessments of specific taxa revealed significant (p<0.05) line by exercise interactions including shifts in the abundances of Firmicutes, Proteobacteria, and Cyanobacteria. Relative abundance of Christensenellaceae family was higher (p = 0.026) in HCR than LCR rats, and positively correlated (p<0.05) with food intake, body weight and running distance. These findings demonstrate that exercise differentially impacts host metabolism and gut microbial communities of female HCR and LCR rats without ovarian function. 相似文献
89.
Klonowska A Clark ME Thieman SB Giles BJ Wall JD Fields MW 《Applied microbiology and biotechnology》2008,78(6):1007-1016
Desulfovibrio vulgaris Hildenborough is a well-studied sulfate reducer that can reduce heavy metals and radionuclides [e.g., Cr(VI) and U(VI)].
Cultures grown in a defined medium had a lag period of approximately 30 h when exposed to 0.05 mM Cr(VI). Substrate analyses
revealed that although Cr(VI) was reduced within the first 5 h, growth was not observed for an additional 20 h. The growth
lag could be explained by a decline in cell viability; however, during this time small amounts of lactate were still utilized
without sulfate reduction or acetate formation. Approximately 40 h after Cr exposure (0.05 mM), sulfate reduction occurred
concurrently with the accumulation of acetate. Similar amounts of hydrogen were produced by Cr-exposed cells compared to control
cells, and lactate was not converted to glycogen during non-growth conditions. D. vulgaris cells treated with a reducing agent and then exposed to Cr(VI) still experienced a growth lag, but the addition of ascorbate
at the time of Cr(VI) addition prevented the lag period. In addition, cells grown on pyruvate displayed more tolerance to
Cr(VI) compared to lactate-grown cells. These results indicated that D. vulgaris utilized lactate during Cr(VI) exposure without the reduction of sulfate or production of acetate, and that ascorbate and
pyruvate could protect D. vulgaris cells from Cr(VI)/Cr(III) toxicity.
J.D. Wall and M.W. Fields are both affiliated to the Virtual Institute of Microbial Stress and Survival ().
M.E. Clark and S.B. Thieman contributed equally to this work. 相似文献
90.
Lipids revert inert Abeta amyloid fibrils to neurotoxic protofibrils that affect learning in mice 总被引:1,自引:0,他引:1 下载免费PDF全文
Martins IC Kuperstein I Wilkinson H Maes E Vanbrabant M Jonckheere W Van Gelder P Hartmann D D'Hooge R De Strooper B Schymkowitz J Rousseau F 《The EMBO journal》2008,27(1):224-233
Although soluble oligomeric and protofibrillar assemblies of Abeta-amyloid peptide cause synaptotoxicity and potentially contribute to Alzheimer's disease (AD), the role of mature Abeta-fibrils in the amyloid plaques remains controversial. A widely held view in the field suggests that the fibrillization reaction proceeds 'forward' in a near-irreversible manner from the monomeric Abeta peptide through toxic protofibrillar intermediates, which subsequently mature into biologically inert amyloid fibrils that are found in plaques. Here, we show that natural lipids destabilize and rapidly resolubilize mature Abeta amyloid fibers. Interestingly, the equilibrium is not reversed toward monomeric Abeta but rather toward soluble amyloid protofibrils. We characterized these 'backward' Abeta protofibrils generated from mature Abeta fibers and compared them with previously identified 'forward' Abeta protofibrils obtained from the aggregation of fresh Abeta monomers. We find that backward protofibrils are biochemically and biophysically very similar to forward protofibrils: they consist of a wide range of molecular masses, are toxic to primary neurons and cause memory impairment and tau phosphorylation in mouse. In addition, they diffuse rapidly through the brain into areas relevant to AD. Our findings imply that amyloid plaques are potentially major sources of soluble toxic Abeta-aggregates that could readily be activated by exposure to biological lipids. 相似文献