全文获取类型
收费全文 | 191篇 |
免费 | 17篇 |
国内免费 | 2篇 |
出版年
2022年 | 2篇 |
2021年 | 2篇 |
2019年 | 4篇 |
2018年 | 2篇 |
2017年 | 3篇 |
2016年 | 4篇 |
2015年 | 6篇 |
2014年 | 6篇 |
2013年 | 5篇 |
2012年 | 13篇 |
2011年 | 16篇 |
2010年 | 5篇 |
2009年 | 5篇 |
2008年 | 6篇 |
2007年 | 8篇 |
2006年 | 4篇 |
2005年 | 8篇 |
2004年 | 3篇 |
2003年 | 11篇 |
2002年 | 3篇 |
2001年 | 10篇 |
2000年 | 3篇 |
1999年 | 4篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1995年 | 4篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1989年 | 6篇 |
1986年 | 3篇 |
1984年 | 3篇 |
1982年 | 3篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 1篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1969年 | 3篇 |
1968年 | 5篇 |
1966年 | 2篇 |
1964年 | 2篇 |
排序方式: 共有210条查询结果,搜索用时 31 毫秒
201.
202.
203.
The hairpin ribozyme 总被引:4,自引:0,他引:4
Richard Shippy Randy Lockner Margaret Farnsworth Arnold Hampel 《Molecular biotechnology》1999,12(1):117-129
The hairpin ribozyme is a member of a family of small RNA endonucleases, which includes hammer-head, human hepatitis delta virus, Neurospora VS, and the lead-dependent catalytic RNAs. All these catalytic RNAs reversibly cleave the phosphodiester bond of substrate RNA to generate 5'-hydroxyl and 2',3'-cyclic phosphate termini. Whereas the reaction products from family members are similar, large structural and mechanistic differences exist. Structurally the hairpin ribozyme has two principal domains that interact to facilitate catalysis. The hairpin ribozyme uses a catalytic mechanism that does not require metals for cleavage or ligation of substrate RNA. In this regard it is presently unique among RNA catalysts. Targeting rules for cleavage of substrate have been determined and required bases for catalysis have been identified. The hairpin ribozyme has been developed and used for gene therapy and was the first ribozyme to be approved for human clinical trials. 相似文献
204.
Eddie Pahuski Mark Klekamp Tom Condon A. E. Hampel 《Journal of cellular physiology》1983,114(1):82-87
The Chinese hamster ovary (CHO) aminoacyl-tRNA synthetase mutants Gln-2, His-1, and Lys-101 were analyzed for alterations in respective particulate enzyme forms. The mutant Gln-2 showed a preferential loss of the lower molecular weight enzyme form for glutamine. His-1 showed alterations of the enzyme complexes for several other aminoacyl-tRNA activities but only decreased activity for itself. The mutant Lys-101 only showed an altered Lysyl-tRNA synthetase. These results provide evidence for a model of the intracellular role of the aminoacyl-tRNA synthetase complexes wherein the high molecular weight forms utilize amino acids directly from the extracellular pool while the low molecular weight forms utilize intracellular pools. 相似文献
205.
Summary Comparable results of chromosomal damage were obtained in human leukocyte cultures by cyclophosphamide after its incubation with liver slices in Warburg vessels. The dose-rate-effects of the activation products and the distribution of 1000 breaks in different chromosome segments were similar to other cyclophosphamide derivatives already analysed. The nonrandom distribution of breaks was statistically significant.
Direktor: Prof. Dr. H. Frhr. von Kress 相似文献
Direktor: Prof. Dr. H. Frhr. von Kress 相似文献
206.
alpha-Aminoadipate pool concentration and penicillin biosynthesis in strains of Penicillium chrysogenum 总被引:1,自引:0,他引:1
W M Jaklitsch W Hampel M R?hr C P Kubicek G Gamerith 《Canadian journal of microbiology》1986,32(6):473-480
Intracellular amino acid pools in four Penicillium chrysogenum strains, which differed in their ability to produce penicillin, were determined under conditions supporting growth without penicillin production and under conditions supporting penicillin production. A significant correlation between the rate of penicillin production and the intracellular concentration of alpha-aminoadipate was observed, which was not shown with any other amino acid in the pool. In replacement cultivation, penicillin production was stimulated by alpha-aminoadipate, but not by valine or cysteine. Exogenously added alpha-aminoadipate (2 or 3 mM) maximally stimulated penicillin synthesis in two strains of different productivity. Under these conditions intracellular concentrations of alpha-aminoadipate were comparable in the two strains in spite of the higher rate of penicillin production in the more productive strain. Results suggest that the lower penicillin titre of strain Q 176 is due to at least two factors: (i) the intracellular concentration of alpha-aminoadipate is insufficient to allow saturation of any enzyme which is rate limiting in the conversion of alpha-aminoadipate to penicillin and (ii) the level of an enzyme, which is rate limiting in the conversion of alpha-aminoadipate to penicillin, is lower in Q 176 (relative to strain D6/1014/A). Results suggest that the intracellular concentration of alpha-aminoadipate in strain D6/1014/A is sufficiently high to allow saturation of the rate-limiting penicillin biosynthetic enzyme in that strain. The basis of further correlation of intracellular alpha-aminoadipate concentration and penicillin titre among strains D6/1014/A, P2, and 389/3, the three highest penicillin producers studied here, remains to be established.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
207.
Rubn Casillas Matthias Adam Pedro B. Coto Andreas R. Waterloo Johannes Zirzlmeier Seelam Rajagopala Reddy Frank Hampel Robert McDonald Rik R. Tykwinski Michael Thoss Dirk M. Guldi 《Liver Transplantation》2019,9(2)
Intermolecular singlet fission (SF) is an electronically coupled process between two chromophores, where distance dependences are decisive in terms of rates and yields. In the current work, a family of pentacene derivatives featuring different functional groups have been designed, synthesized, and probed with respect to intermolecular SF in the low, medium, and high concentration regimes rather than in the solid state. By means of advanced photophysical techniques, global analysis modeling, and ab initio calculations, a model for intermolecular SF is postulated. The model is based on an early key intermediate, which involves the diffusional encounter between one pentacene in its singlet excited‐state with another one in its ground state and which features excimer characteristics. This is followed by a transformation into a coupled triplet excited‐state. The role of the functional group appended to pentacene is analyzed with respect to steric shielding of the pentacene core as a means to prevent photophysical degradation, as well as control diffusional encounter and, subsequently, SF. The findings demonstrate the potential of new molecular materials for SF, especially in solution studies, as well as the challenges of implementing them in energy conversion schemes due to the appearance of photodegradation processes that compete with SF. 相似文献
208.
209.
210.