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31.
Joukar S Bashiri H Dabiri S Ghotbi P Sarveazad A Divsalar K Joukar F Abbaszadeh M 《Journal of physiology and biochemistry》2012,68(2):271-279
The present study was designed to elucidate the outcome of subchronic co-administration of black tea and nicotine on cardiovascular
performance and whether these substances could modulate the isoproterenol-induced cardiac injury. Animal groups were control,
black tea, nicotine and black tea plus nicotine. Test groups received nicotine (2 mg/kg s.c.) and black tea brewed (p.o.)
each alone and in combination for 4 weeks. On the 28th day, myocardial damage was induced by isoproterenol (50 mg/kg i.p.),
and blood samples were taken. On day 29, after hemodynamic parameters recording, hearts were removed for histopathological
evaluation. Tea or nicotine consumption had no significant effects on hemodynamic indices of animals without heart damage.
When the cardiac injury was induced, tea consumption maintained the maximum dp/dt, and nicotine significantly decreased the
pressure–rate product. Moreover, severity of heart lesions was lower in the presence of nicotine or black tea. Concomitant
use of these materials did not show extra effects on mentioned parameters more than the effect of each of them alone. The
results suggest that subchronic administration of black tea or nicotine for a period of 4 weeks may have a mild cardioprotective
effect, while concomitant use of these materials cannot intensify this beneficial effect. 相似文献
32.
Hamideh Ghodrati Azadi Seyed Mahmood Ghaffari Gholam Hossein Riazi Shahin Ahmadian Fatemeh Vahedi 《Cytotechnology》2008,56(3):179-185
Allium hirtifolim (Persian Shallot) belongs to Allium genus (Alliaceae family). We investigated the in vitro effects of chloroformic extract of A. hirtifolium and its Allicin on the proliferation of HeLa (cervical cancer), MCF7 (human, caucasion, breast, adenocarcinoma) and L929
(mouse, C3H/An, connective) cell lines. Our results showed that components of A. hirtifolium might inhibit proliferation of tumor cell lines. This inhibition in HeLa and MCF-7 cells was dose-dependent. The presence
of Allicin was evaluated by TLC method in bulbs and the extract of A. hirtifolium was analyzed by HPLC. MTT test was performed 24, 48 and 72 h after cell culture. A significant decrease in cell lines was
observed in HeLa and MCF-7 as compared to L929 cell lines. DNA fragmentation analysis revealed a large number of apoptotic
cells in treated HeLa and MCF-7 cell groups, but no effects in L929 cells. Therefore A. hirtifolium might be a candidate for tumor suppression. 相似文献
33.
Parisa Taghipour Farhad Zolfagharpour Hamideh Daneshvar Farhood Ziaie 《Luminescence》2022,37(5):742-757
In this research work, pure and doped hydroxyapatite samples were synthesized using hydrolysis and hydrothermal methods to produce powder material. The crystal structure was carried out by producing data using the X-ray diffraction system and the Rietveld method using material analysis using diffraction software. Then the sample was irradiated with different radiation absorbed doses, and their thermoluminescence response was investigated from the dosimetry point of view. The results showed that the synthesis method, doping, and annealing temperature could significantly affect the crystal structure and thermoluminescence dosimetry response of hydroxyapatite samples, consequently. The results showed that the high-temperature annealing process and dopant could lead to the formation of the β-TCP crystal phase during or after the synthesis of hydroxyapatite, and the percentage of this formed phase increased when raising the temperature, and finally led to increase in the thermoluminescence response. 相似文献
34.
Hamideh Kooshki Marzieh Ghollasi Raheleh Halabian Negar M. Kazemi 《Journal of cellular physiology》2019,234(5):5343-5353
Tissue engineering is an interdisciplinary expertise that involves the use of nanoscaffolds for repairing, modifying, and removing tissue defects and formation of new tissues. Mesenchymal stem cells (MSCs) can differentiate into a variety of cell types, and they are attractive candidates for tissue engineering. In the current study, the electrospinning process was used for nanofiber preparation, based on a poly-l -lactic-acid (PLLA) polymer. The surface was treated with O 2 plasma to enhance hydrophilicity, cell attachment, growth, and differentiation potential. The nanoscaffolds were preconditioned with lipopolysaccharide (LPS) to enhance induction of differentiation. The nanoscaffolds were categorized by contact angle measurements and scanning electron microscopy. The MTT assay was used to analyze the rate of growth and proliferation of cells. Osteogenic differentiation of cultured MSCs was evaluated on nanofibers using common osteogenic markers, such as alkaline phosphatase activity, calcium mineral deposition, quantitative real-time polymerase chain reaction, and immunocytochemical analysis. Based on the in vitro results, primed MSCs with LPS on the PLLA nanoscaffold significantly enhanced the proliferation and osteogenesis of MSCs. Also, the combination of LPS and electrospun nanofibers can provide a new and suitable matrix to support stem cells’ differentiation for bone tissue engineering. 相似文献
35.
The crosstalk between trace elements with DNA damage response,repair, and oxidative stress in cancer
Sadra Samavarchi Tehrani Hamideh Mahmoodzadeh Hosseini Tooba Yousefi Maryam Abolghasemi Durdi Qujeq Mahmood Maniati Jafar Amani 《Journal of cellular biochemistry》2019,120(2):1080-1105
DNA damage response (DDR) is a regulatory system responsible for maintaining genome integrity and stability, which can sense and transduce DNA damage signals. The severity of damage appears to determine DDRs, which can include damage repair, cell-cycle arrest, and apoptosis. Furthermore, defective components in DNA damage and repair machinery are an underlying cause for the development and progression of various types of cancers. Increasing evidence indicates that there is an association between trace elements and DDR/repair mechanisms. In fact, trace elements seem to affect mediators of DDR. Besides, it has been revealed that oxidative stress (OS) and trace elements are associated with cancer development. In this review, we discuss the role of some critical trace elements in the risk of cancer. In addition, we provide a brief introduction on DDR and OS in cancer. Finally, we will further review the interactions between some important trace elements including selenium, zinc, chromium, cadmium, and arsenic, and DDR, and OS in cancer. 相似文献
36.
Three new five-coordinate CuII complexes, [Cu(tpy)(phen-dione)](PF6)2, [Cu(phen)(phen-dione)Cl]PF6 and [Cu(bpy)(phen-dione)Cl]PF6 (tpy = 2,2′;6′,2″-terpyridine, phen = 1,10-phenanthroline and phen-dione = 1,10-phenanthroline-5,6-dione) have been prepared and characterized by elemental analysis, IR and UV-Vis spectroscopies and cyclic voltammetry.The complex of [Cu(tpy)(phen-dione)](PF6)2 crystallized with one molecule of acetonitrile. The ortep drawing of [Cu(tpy)(phen-dione)](PF6)2 · CH3CN shows that the coordination geometry around CuII is a distorted trigonal- bipyramid. Due to the steric hindrance of in the unit cell, the tpy ligands in each complex cation cannot interact in a π-π fashion. The effective magnetic moment (μeff) of the complexes was measured by the Evans method. The cyclic voltammograms at Pt disk electrode for these complexes display only one reversible Cu(II)/Cu(I) redox couple. 相似文献
37.
Salahshourifar I Gilani MA Vosough A Tavakolzadeh T Tahsili M Mansori Z Karimi H Totonchi M Gourabi H 《Journal of applied genetics》2007,48(1):93-94
Complex Chromosomal Rearrangements (CCRs) are rare structural abnormalities that are usually associated with infertility or subfertility in male carriers. We described clinical and chromosomal features of a non-obstructive azoospermic male that has been referred for infertility. Cytogenetic analysis showed three chromosomes, i.e. 3, 8 and 16, which have been involved and caused spermatogenesis failure. 相似文献
38.
Hamid Moghimi Javad Hamedi Zargham Sepehrizadeh Hamideh Ofoghi 《Annals of microbiology》2013,63(2):669-675
Weeds are a significant part of the pests limiting crop production. Currently, chemical herbicides are widely used for weed control. Environment pollution and the rise of resistant strains highlight the need for new herbicides. Nep1 is a natural bio-herbicide protein which is an effective necrosis stimulant in dicotyledonous weeds. In this study, the cDNA encoding nep1 was isolated form Fusarium oxysporum, cloned and overexpressed in Escherichia coli. The Nep1 inclusion body was purified and refolded. For biological assay, the recombinant Nep1 was applied on Sinapis arvensis, as a chemical herbicide-resistant weed, and on Nicotiana tabacum, as a model plant. Our results show a significant necrosis on the leaves of S. arvensis and N. tabacum after spraying 50 μg/ml of the recombinant protein. 相似文献
39.
Abozar Najafi Hossein Daghigh Kia Hossein Mohammadi Mir Hossein Najafi Zaynab Zanganeh Mohsen Sharafi Felipe Martinez-Pastor Hamideh Adeldust 《Cryobiology》2014
The aim of this study was to evaluate the effects of ergothioneine and cysteamine as antioxidant supplements in a soybean lecithin extender for freezing ram semen. Twenty-four ejaculates were collected from four rams and diluted with extenders (1.5% soybean lecithin, 7% glycerol) containing no supplements (control) and cysteamine or ergothioneine (2, 4, 6 or 8 mM). Motility by CASA, viability, plasma membrane functionality (HOS test), total abnormality, lipid peroxidation, glutathione peroxidase (GPx) activity and capacitation status (CTC staining) were assessed after thawing. Using 6 mM of either antioxidant improved total motility. Cysteamine at 6 mM and ergothioneine at 4 and 6 mM improved viability and reduced lipid peroxidation (malondialdehyde concentration). Both antioxidants improved membrane functionality significantly, except at 8 mM. Progressive motility, kinematic parameters, GPx activity, capacitation status and sperm abnormalities were not influenced by the antioxidant supplements. In conclusion, cysteamine at 6 mM and ergothioneine at 4 or 6 mM seem to improve the post-thawing quality of ram semen cryopreserved in a soybean lecithin extender. 相似文献
40.
Ahad Mokhtarzadeh Hamideh Parhiz Maryam Hashemi Sara Ayatollahi Khalil Abnous Mohammad Ramezani 《AAPS PharmSciTech》2015,16(5):1025-1032
Specific and effective delivery of drugs and genes to cancer cells are
the major issues in successful cancer treatment. Recently, targeted cancer gene
therapy has been emerged as a main technology for the treatment of different types
of cancers. Among various synthetic carriers, polyethylenimine is one of the most
well-known polymers for gene delivery. In this study, we conjugated phage-derived
peptide (DMPGTVLP) to polyethylenimine (10 kDa) via disulfide bonds for targeted gene delivery into breast
adenocarcinoma cells (MCF-7). As negative-control cells, we used non-related
hepatocellular carcinoma cells (HepG2). Peptide-conjugated polyplex exhibited low
cytotoxicity and significantly increased the transfection efficiency in comparison
with unmodified polyethylenimine. Therefore, the peptide-modified vector can be used
as a good targeting agent for gene or drug delivery into breast adenocarcinoma
cells.