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排序方式: 共有148条查询结果,搜索用时 203 毫秒
91.
Donohoe BS Vinzant TB Elander RT Pallapolu VR Lee YY Garlock RJ Balan V Dale BE Kim Y Mosier NS Ladisch MR Falls M Holtzapple MT Sierra-Ramirez R Shi J Ebrik MA Redmond T Yang B Wyman CE Hames B Thomas S Warner RE 《Bioresource technology》2011,102(24):11097-11104
The US Department of Energy-funded Biomass Refining CAFI (Consortium for Applied Fundamentals and Innovation) project has developed leading pretreatment technologies for application to switchgrass and has evaluated their effectiveness in recovering sugars from the coupled operations of pretreatment and enzymatic hydrolysis. Key chemical and physical characteristics have been determined for pretreated switchgrass samples. Several analytical microscopy approaches utilizing instruments in the Biomass Surface Characterization Laboratory (BSCL) at the National Renewable Energy Laboratory (NREL) have been applied to untreated and CAFI-pretreated switchgrass samples. The results of this work have shown that each of the CAFI pretreatment approaches on switchgrass result in different structural impacts at the plant tissue, cellular, and cell wall levels. Some of these structural changes can be related to changes in chemical composition upon pretreatment. There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields. 相似文献
92.
In the previous issue of Arthritis Research & Therapy, Ducourau and colleagues report that they retrospectively detected anti-infliximab antibodies in 21% of patients with rheumatic
diseases. Patients with anti-infliximab antibodies had lower serum drug concentrations. These findings contribute to the existing
evidence of immunogenicity of biologicals and its clinical relevance. We argue for therapeutic drug monitoring to optimize
treatment response. 相似文献
93.
Raymond Hames 《Human ecology: an interdisciplinary journal》1983,11(1):13-34
A number of researchers have suggested that polyculture is characteristic of native tropical forest swiddens and have adduced theory from community ecology to account for its adaptiveness. Ye'kwana and Yanomamö swidden cultivation is examined, and it is shown that polyculture is not practiced to any significant degree. Instead, the concept of polyvariety is introduced along with a number of other cultivation practices that more simply account for the adaptiveness of Ye'kwana and Yanomamö gardening. In addition, comparative data from other parts of the tropical world indicate that polyculture is no more common than monoculture and recent advances in ecological research indicate that the diversity-stability hypothesis that underpins adaptive arguments of polyculture is in need of drastic revision. 相似文献
94.
Alexandre C Motta Joost LM Vissers Renée Gras Betty CAM Van Esch Antoon JM Van Oosterhout Martijn C Nawijn 《Respiratory research》2009,10(1):1-8
Background
Anxiety and depression are common and treatable risk factors for re-hospitalisation and death in patients with COPD. The degree of lung function impairment does not sufficiently explain anxiety and depression. The BODE index allows a functional classification of COPD beyond FEV1. The aim of this cross-sectional study was (1) to test whether the BODE index is superior to the GOLD classification for explaining anxious and depressive symptoms; and (2) to assess which components of the BODE index are associated with these psychological aspects of COPD.Methods
COPD was classified according to the GOLD stages based on FEV1%predicted in 122 stable patients with COPD. An additional four stage classification was constructed based on the quartiles of the BODE index. The hospital anxiety and depression scale was used to assess anxious and depressive symptoms.Results
The overall prevalence of anxious and depressive symptoms was 49% and 52%, respectively. The prevalence of anxious symptoms increased with increasing BODE stages but not with increasing GOLD stages. The prevalence of depressive symptoms increased with both increasing GOLD and BODE stages. The BODE index was superior to FEV1%predicted for explaining anxious and depressive symptoms. Anxious symptoms were explained by dyspnoea. Depressive symptoms were explained by both dyspnoea and reduced exercise capacity.Conclusion
The BODE index is superior to the GOLD classification for explaining anxious and depressive symptoms in COPD patients. These psychological consequences of the disease may play a role in future classification systems of COPD. 相似文献95.
MT Butcher JW Hermanson NG Ducharme LM Mitchell LV Soderholm JE Bertram 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2009,152(1):100-114
The forelimb digital flexors of the horse display remarkable diversity in muscle architecture despite each muscle-tendon unit having a similar mechanical advantage across the fetlock joint. We focus on two distinct muscles of the digital flexor system: short compartment deep digital flexor (DDF(sc)) and the superficial digital flexor (SDF). The objectives were to investigate force-length behavior and work performance of these two muscles in vivo during locomotion, and to determine how muscle architecture contributes to in vivo function in this system. We directly recorded muscle force (via tendon strain gauges) and muscle fascicle length (via sonomicrometry crystals) as horses walked (1.7 m s(-1)), trotted (4.1 m s(-1)) and cantered (7.0 m s(-1)) on a motorized treadmill. Over the range of gaits and speeds, DDF(sc) fascicles shortened while producing relatively low force, generating modest positive net work. In contrast, SDF fascicles initially shortened, then lengthened while producing high force, resulting in substantial negative net work. These findings suggest the long fibered, unipennate DDF(sc) supplements mechanical work during running, whereas the short fibered, multipennate SDF is specialized for economical high force and enhanced elastic energy storage. Apparent in vivo functions match well with the distinct architectural features of each muscle. 相似文献
96.
Dynamic recruitment of Nek2 kinase to the centrosome involves microtubules, PCM-1, and localized proteasomal degradation 下载免费PDF全文
Hames RS Crookes RE Straatman KR Merdes A Hayes MJ Faragher AJ Fry AM 《Molecular biology of the cell》2005,16(4):1711-1724
Centrosomes undergo dramatic changes in composition and activity during cell cycle progression. Yet mechanisms involved in recruiting centrosomal proteins are poorly understood. Nek2 is a cell cycle-regulated protein kinase required for regulation of centrosome structure at the G2/M transition. Here, we have addressed the processes involved in trafficking of Nek2 to the centrosome of human adult cells. We find that Nek2 exists in small, highly dynamic cytoplasmic particles that move to and from the centrosome. Many of these particles align along microtubules and a motif was identified in the Nek2 C-terminal noncatalytic domain that allows both microtubule binding and centrosome localization. FRAP experiments reveal that 70% of centrosomal Nek2 is rapidly turned over (t(1/2) approximately 3 s). Microtubules facilitate Nek2 trafficking to the centrosome but only over long distances. Cytoplasmic Nek2 particles colocalize in part with PCM-1 containing centriolar satellites and depletion of PCM-1 interferes with centrosomal recruitment of Nek2 and its substrate C-Nap1. Finally, we show that proteasomal degradation is necessary to allow rapid recruitment of new Nek2 molecules to the centrosome. Together, these data highlight multiple processes involved in regulating the abundance of Nek2 kinase at the centrosome including microtubule binding, the centriolar satellite component PCM-1, and localized protein degradation. 相似文献
97.
The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis 下载免费PDF全文
Wilson GM Fielding AB Simon GC Yu X Andrews PD Hames RS Frey AM Peden AA Gould GW Prekeris R 《Molecular biology of the cell》2005,16(2):849-860
An integral part of cell division is the separation of daughter cells via cytokinesis. There is now good evidence that the completion of cytokinesis requires coordinated membrane trafficking to deliver new membrane to the tip of the furrow and to complete the abscission. Here we have examined membrane traffic in cytokinesis and describe several novel observations. First, we show that Rab11- and FIP3-containing recycling endosomes accumulate near the cleavage furrow and are required for successful completion of cytokinesis. Second, we demonstrate that the Rab11-FIP3 protein complex is intimately involved in the delivery of endosomes to the cleavage furrow. Significantly, although FIP3 recruitment to endosomes is Rab11 dependent, we find that the targeting of FIP3 to the midbody is independent of Rab11. Third, we show that the Rab11-FIP3 complex is required for a late stage of cytokinesis, possibly abscission. Finally, we demonstrate that localization of FIP3 is subject to substantial spatial and temporal regulation. These data provide the first detailed analysis of recycling endosomes in cell division and provide a new model for membrane traffic to the furrow. We propose that the dynamic Rab11-FIP3 interaction controls the delivery, targeting, and fusion of recycling endosomes with furrow during late cytokinesis and abscission. 相似文献
98.
The precise quantitative analysis of biomass derived sugars is a very important step in the conversion of biomass feedstocks to fuels and chemicals. However, the most accurate method of biomass sugar analysis is based on the gas chromatography analysis of derivatized sugars either as alditol acetates or trimethylsilanes. The derivatization method is time-consuming but the alternative HPLC method cannot resolve most sugars found in biomass hydrolysates. We have demonstrated for the first time that by careful manipulation of the HPLC mobile phase, biomass monomeric sugars (arabinose, xylose, fructose, glucose, mannose, and galactose) can be analyzed quantitatively and there is excellent baseline resolution of all the sugars. This was demonstrated for both standard sugars and corn stover hydrolysates. Our method can also be used to analyze dimmeric sugars (cellobiose and sucrose). 相似文献
99.
The major spore coat proteins of Dictyostelium discoideum are synthesized during the culmination stage of development. In an attempt to examine the regulatory mechanisms involved, spore coat protein synthesis by pseudoplasmodia harvested prior to culmination and incubated in submerged culture under various environmental conditions has been monitored. It is reported that the synthesis of spore coat proteins SP170, SP103, SP94, SP82, SP76, and SP55 is dependent upon the presence of a low-molecular-weight (Mr approx 100), heat-stable factor secreted by cells incubated at high density in buffer. Previous studies have implicated cyclic AMP, ammonia, and amino acids in spore cell differentiation. Partial purification of the spore coat protein inducing factor (SPIF), together with attempts to mimic its activity, indicate that SPIF is not identical with any of these molecules and it is probably also distinct from DIF and "fruit juice," two other factors which regulate the spore-stalk decision and the initiation of culmination, respectively, in D. discoideum. In addition to SPIF, the continued expression of the spore coat protein genes also requires that the integrity of the pseudoplasmodium be maintained. Unlike the expression of many other genes after aggregation, this latter requirement cannot be replaced by exogenous cyclic AMP. Termination of spore coat protein gene expression occurs despite the presence of excess exogenous SPIF and hence involves mechanisms other than the destruction or depletion of SPIF. 相似文献
100.
Yonekawa H; Moriwaki K; Gotoh O; Miyashita N; Matsushima Y; Shi LM; Cho WS; Zhen XL; Tagashira Y 《Molecular biology and evolution》1988,5(1):63-78
The Japanese mouse, Mus musculus molossinus, has long been considered an
independent subspecies of the house mouse. A survey of restriction- site
haplotypes of mitochondrial DNA (mtDNA) showed that Japanese mice have two
main maternal lineages. The most common haplotype is closely related to the
mtDNA of the European subspecies M. m. musculus. The other common haplotype
and two minor ones are closely related to each other and to the mtDNA of an
Asiatic subspecies, M. m. castaneus. Two other rare variants are probably
the result of recent contamination by European M. m. domesticus. The
musculus type of mtDNA is found in the southern two-thirds of Japan,
whereas the common castaneus type is found in the northern third and the
minor variants are found sporadically throughout Japan. The castaneus mtDNA
lineage had a few minor variants, whereas the musculus lineage was
completely monomorphic. By contrast, the native population of M. m.
castaneus and the Chinese and Korean musculus populations were highly
polymorphic. These results suggest that M. m. molossinus is a hybrid
between ancestral colonies, possibly very small, of M. m. musculus and M.
m. castaneus, rather than an independent subspecies.
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