排序方式: 共有156条查询结果,搜索用时 31 毫秒
51.
Markus Pfister Holger Thiele Guy Van Camp Erik Fransen Fazil Apaydin Omer Aydin Peter Leistenschneider Marcella Devoto Hans-Peter Zenner Nikolaus Blin Peter Nürnberg Haluk Ozkarakas Susan Kupka 《Cellular physiology and biochemistry》2004,14(4-6):369-376
BACKGROUND: Alpha-tectorin is a noncollagenous component of the tectorial membrane which plays an essential role in auditory transduction. In several DFNA12 families mutations in TECTA, the gene encoding alpha-tectorin, were shown to cause hearing impairment (HI) with different phenotypes depending on the location of the mutation. METHODS/RESULTS: Here we report a Turkish family displaying autosomal dominant inherited HI. Linkage analysis revealed significant cosegregation (LOD score: 4.6) of the disease to markers on chromosome 11q23.3- q24. This region contains the TECTA gene which was subsequently sequenced. A nucleotide change in exon 13, 4526T>G, was detected leading to a substitution from cysteine to glycine at codon 1509 of the TECTA protein. This cysteine is located in vWFD4 domain, a protein domain which is supposed to be involved in disulfide bonds and protein-protein interactions. CONCLUSIONS: It is conspicuous that the phenotype in this family correlates with other families, also displaying mutations involving conserved cysteines. In all three families these mutations result in progressive HI involving high frequencies. In contrast, mutations which are not affecting the vWFD domains seem to provoke mid-frequency sensorineural HI. Furthermore, evaluation of clinical data in our family revealed a gender effect for the severity of hearing impairment. Males were significantly more affected than females. The identification of the third family displaying a missense mutation in the vWFD domain of alpha-tectorin underlines the phenotype-genotype correlation based on different mutations in TECTA. 相似文献
52.
The effect of various culture conditions on the levels of ammonia assimilatory enzymes of Corynebacterium callunae 总被引:2,自引:0,他引:2
Haluk Ertan 《Archives of microbiology》1992,158(1):42-47
Corynebacterium callunae (NCIB 10338) grows faster on glutamate than ammonia when used as sole nitrogen sources. The levels of glutamine synthetase (GS; EC 6.3.1.2) and glutamate synthase (GOGAT; EC 1.4.1.13) of C. callunae were found to be influenced by the nitrogen source. Accordingly, the levels of GS and GOGAT activities were decreased markedly under conditions of ammonia excess and increased under low nitrogen conditions. In contrast, glutamate dehydrogenase (GDH; EC 1.4.1.4) activities were not significantly affected by the type or the concentration of the nitrogen source supplied. The carbon source in the growth medium could also affect GDH, GS and GOGAT levels. Of the carbon sources tested in the presence of 2 mM or 10 mM ammonium chloride as the nitrogen source pyruvate, acetate, fumarate and malate caused a decrease in the levels of all three enzymes as compared with glucose. GDH, GS and GOGAT levels were slightly influenced by aeration. Also, the enzyme levels varied with the growth phase. Methionine sulfoximine, an analogue of glutamine, markedly inhibited both the growth of C. callunae cells and the transferase activity of GS. The apparent K
m values of GDH for ammonia and glutamate were 17.2 mM and 69.1 mM, respectively. In the NADPH-dependent reaction of GOGAT, the apparent K
m values were 0.1 mM for -ketoglutarate and 0.22 mM for glutamine.Abbreviations GDH
glutamate dehydrogenase
- GS
glutamine synthetase
- GOGAT
glutamate synthase 相似文献
53.
Some properties of glutamate dehydrogenase,glutamine synthetase and glutamate synthase from Corynebacterium callunae 总被引:4,自引:0,他引:4
Haluk Ertan 《Archives of microbiology》1992,158(1):35-41
Characteristics of the three major ammonia assimilatory enzymes, glutamate dehydrogenase (GDH), glutamine synthetase (GS) and glutamate synthase (GOGAT) in Corynebacterium callunae (NCIB 10338) were examined. The GDH of C. callunae specifically required NADPH and NADP+ as coenzymes in the amination and deamination reactions, respectively. This enzyme showed a marked specificity for -ketoglutarate and glutamate as substrates. The optimum pH was 7.2 for NADPH-GDH activity (amination) and 9.0 for NADP+-GDH activity (deamination). The results showed that NADPH-GDH and NADP+-GDH activities were controlled primarily by product inhibition and that the feedback effectors alanine and valine played a minor role in the control of NADPH-GDH activity. The transferase activity of GS was dependent on Mn+2 while the biosynthetic activity of the enzyme was dependent on Mg2+ as essential activators. The pH optima for transferase and biosynthetic activities were 8.0 and 7.0, respectively. In the transfer reaction, the K
m values were 15.2 mM for glutamine, 1.46 mM for hydroxylamine, 3.5×10-3 mM for ADP and 1.03 mM for arsenate. Feedback inhibition by alanine, glycine and serine was also found to play an important role in controlling GS activity. In addition, the enzyme activity was sensitive to ATP. The transferase activity of the enzyme was responsive to ionic strength as well as the specific monovalent cation present. GOGAT of C. callunae utilized either NADPH or NADH as coenzymes, although the latter was less effective. The enzyme specifically required -ketoglutarate and glutamine as substrates. In cells grown in a medium with glutamate as the nitrogen source, the optimum pH was 7.6 for NADPH-GOGAT activity and 6.8 for NADH-GOGAT activity. Findings showed that NADPH-GOGAT and NADH-GOGAT activities were controlled by product inhibition caused by NADP+ and NAD+, respectively, and that ATP also had an important role in the control of NADPH-GOGAT activity. Both activities of GOGAT were found to be inhibited by azaserine.Abbreviations GDH
glutamate dehydrogenase
- GOGAT
glutamate synthase
- GS
glutamine synthetase 相似文献
54.
55.
Lehr S Kotzka J Avci H Sickmann A Meyer HE Herkner A Muller-Wieland D 《Biochemistry》2004,43(38):12133-12140
Gab1 (Grb2-associated binder1) belongs to a family of multifunctional docking proteins that play a central role in the integration of receptor tyrosine kinase (RTK) signaling, i.e., mediating cellular growth response, transformation, and apoptosis. In addition to RTK-specific tyrosine phosphorylation, these docking proteins also can be phosphorylated on serine/threonine residues affecting signal transduction. Since serine and threonine phosphorylation are capable of modulating the initial signal one major task to elucidate signal transduction via Gab1 is to determine the exact localization of distinct phosphorylation sites. To address this question in this report we examined extracellular signal-regulated kinases 1/2 (ERK) specific serine/threonine phosphorylation of the entire Gab1 engaged in insulin signaling in more detail in vitro. To elucidate the ERK1/2-specific phosphorylation pattern of Gab1, we used phosphopeptide mapping by two-dimensional HPLC analysis. Subsequently, phosphorylated serine/threonine residues were identified by sequencing the separated phosphopeptides using matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and Edman degradation. Our results demonstrate that ERK1/2 phosphorylate Gab1 at six serine/threonine residues (T312, S381, S454, T476, S581, S597) in consensus motifs for MAP kinase phosphorylation. Serine residues S454, S581, S597, and threonine residue T476 represent nearly 80% of overall incorporated phosphate. These sites are located adjacent to src homology region-2 (SH2) binding motifs (YVPM-motif: Y447, Y472, Y619) specific for the phosphatidylinositol 3kinase (PI3K). The biological role of identified phosphorylation sites was proven by PI3K and Akt activity in intact cells. These data demonstrate that ERK1/2 modulate insulin action via Gab1 by targeting serine and threonine residues beside YXXM motifs. Accordingly, insulin signaling is blocked at the level of PI3K. 相似文献
56.
57.
We analyze the dynamic properties of a neural network model for on-off spiking neurons recorded in the first optic chiasm
of the fly visual system. The model consists of two parallel pathways and three sequential processing stages. The first stage
models photoreceptors. At the second stage, the signal is segregated into on- and off-pathways. These pathways are proposed
to correspond to two populations of amacrine cells. At the third stage, the on- and off-pathways converge to on-off neurons.
Furthermore, according to the model, on-off neurons interact via recurrent connections. This stage is proposed to correspond
to lamina L4 neurons. In response to luminance increments and decrements, the model exhibits a three-component response and
suggests pathways for each of the components. When stimulated by a train of pulses, the model exhibits fast adaptation for
frequencies higher than about 5 Hz. Furthermore, adaptation to on- and off-pulses occurs independently. When the frequency
of stimulation is reduced, the unit recovers rapidly from its adapted state. The temporal modulation transfer function has
its peak around 7 Hz. The phase characteristics show a phase lead for low temporal frequencies changing to a phase lag for
high frequencies. These model predictions are compared with data from Jansonius and van Hateren (1991).
Received: 26 May 1997 / Accepted in revised form: 19 February 1998 相似文献
58.
ule
eker Haluk Beyenal Fatma Ayhan Abdurrahman Tanyola 《Enzyme and microbial technology》1998,23(7-8):469-474
In this study, microbial production of rennin, a milk-clotting enzyme, from a commercial strain of Mucor miehei NRRL 3420 has been investigated in a continuously fed fermenter for prolonged times. The spherical film-type growth of the culture has been accomplished in the fermenter and the effects of medium pH, mixing and dilution rates, and feed
-glucose concentration on milk-clotting activity have been elaborated. In the fermenter, optimum operational parameters have been determined as 400 rpm, 0.125 day−1, and 7.5 g l−1 for mixing rate, dilution rate, and feed
-glucose concentration, respectively. Under these conditions, the fermenter operated 575 h continuously producing 1.24 IU ml−1 maximum milk-clotting activity without concentration. In the fermenter sample at maximum milk clotting activity, the R factor and specific milk-clotting activity were determined as 1.55 × 10−3 IU PU−1 and 5.28 IU mg−1 medium protein, respectively, denoting competitive characteristics of a commercial rennet after concentration. 相似文献
59.