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排序方式: 共有254条查询结果,搜索用时 15 毫秒
51.
Zhang Yang Adnan Halim Yoshiki Narimatsu Hiren Jitendra Joshi Catharina Steentoft Katrine Ter-Borch Gram Schjoldager Morten Alder Schulz Natalie R. Sealover Kevin J. Kayser Eric Paul Bennett Steven B. Levery Sergey Y. Vakhrushev Henrik Clausen 《Molecular & cellular proteomics : MCP》2014,13(12):3224-3235
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Vincentius A. Halim Alexander Muck Markus Hartl Alfredo J. Ibáñez Ashok Giri Florian Erfurth Ian T. Baldwin Aleš Svatoš Dr. 《Proteomics》2009,9(1):171-181
Being able to rapidly and sensitively detect specific enzymatic products is important when screening biological samples for enzymatic activity. We present a simple method for assaying protease activity in the presence of protease inhibitors (PIs) by measuring tryptic peptide accumulation on copolymer pMALDI target chips using a dual fluorescence/MALDI‐TOF‐MS read‐out. The small platform of the chip accommodates microliter amounts of sample and allows for rapid protein digestion. Fluorescamine labeling of tryptic peptides is used to indicate the proteolytic activity and is shown to be an affordable, simple process, yielding a strong fluorescence signal with a low background. Subsequent MALDI‐TOF‐MS analysis, performed in the same sample well, or in a parallel well without adding fluorescamine, detects the specific tryptic peptides and provides confidence in the assay. The dual read‐out method was applied to screen the inhibition activity of plant PIs, components of plant defense against herbivores and pathogens. Extracts of PIs from Solanum nigrum and trypsin were applied together to a pMALDI chip on which a suitable substrate was adsorbed. The fluorescence and MALDI‐TOF‐MS signal decrease were associated with the inhibitory effect of the PIs on trypsin. The developed platform can be modified to screen novel protease inhibitors, namely, those potentially useful for treating or preventing infection by viruses, including HIV and hepatitis C. 相似文献
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Mukhtar Ahmed Daoud Ali Abdel Halim Harrath Tajamul Hussain Nasser Al-Daghri Majed S. Alokail Ravindranath H. Aladakatti Mukhtar Ahmed G. Ghodesawar 《Comptes rendus biologies》2014,337(4):250-257
Boswellia papyrifera and Boswellia carterii diffuses smoke polluting air that adversely affects indoor environment that certainly harm human health. Therefore, this study aims at ascertaining the effect of these plants on gonadal hormones and molecular changes in rat spermatozoa. The animals were exposed to 4 g/kg body weight of B. papyrifera and B. carterii daily for 120 days along with suitable controls. Significant decreases in FSH, LH and testosterone levels were evidenced, along with a reduction of protein, sialic acid, and carnitine levels. In sperm physiology, sperm count, motility, speed decrease, whereas sperm anomalies increase. TEM observation indicates morphological changes in plasma and acrosomal membranes, cytoplasmic droplet in the tail region, vacuolated, and disorganization of the mitochondrial sheath. These findings demonstrate that B. papyrifera and B. carterii smoke affects the process of sperm formation and maturation, which indicates the detrimental effects of these plants on the reproductive system. 相似文献
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A molecular analysis of the phylogenetic position of the suborder Cavernicola within the Tricladida (Platyhelminthes), with the description of a new species of stygobiont flatworm from Benin
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Abdel Halim Harrath Lamjed Mansour Moïssou Lagnika Ronald Sluys Claude Boutin Saleh Alwasel Arnau Poch Marta Riutort 《Zoological Journal of the Linnean Society》2016,178(3):482-491
This paper presents a new species of cavernicolan planarian from the African continent, which enabled us to determine the position of the suborder Cavernicola within the order Tricladida on a molecular basis. A recent paper suggested a sister‐group relationship between Cavernicola and marine triclads. However, our work is the first molecular study to demonstrate unequivocally that the Cavernicola does not group with either the freshwater planarians or the land planarians, i.e. is not closely related to the suborder Continenticola, and corroborates the suggested sister‐group relationship with the marine triclads or Maricola. Two possible scenarios are proposed for the evolution of the Cavernicola from marine ancestors. The new species is assigned to the genus Novomitchellia and is characterized by the absence of eyes. Testicular follicles are numerous and only dorsal, extending from behind the ovaries to the posterior end of the body. The vasa deferentia separately penetrate the penis bulb and the seminal vesicle. The penis papilla is very short and blunt, and the penis bulb musculature is weak. A long and narrow copulatory bursa is situated behind the gonopore. The common oviduct is orientated perpendicularly to the short bursal canal. 相似文献
56.
Conformational characterization of the intrinsically disordered protein Chibby: Interplay between structural elements in target recognition
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Goh Kar Shin Ng Zhang Jin Halim Murni Oslan Siti Nurbaya Oslan Siti Nur Hazwani Tan Joo Shun 《International journal of peptide research and therapeutics》2022,28(2):1-15
International Journal of Peptide Research and Therapeutics - Chikungunya virus is a major arbovirus of great public health concern in the whole world, but no vaccine is yet available, still advance... 相似文献
60.
Gras S Wilmann PG Chen Z Halim H Liu YC Kjer-Nielsen L Purcell AW Burrows SR McCluskey J Rossjohn J 《Journal of immunology (Baltimore, Md. : 1950)》2012,188(1):311-321
EBV is a ubiquitous and persistent human pathogen, kept in check by the cytotoxic T cell response. In this study, we investigated how three TCRs, which differ in their T cell immunodominance hierarchies and gene usage, interact with the same EBV determinant (FLRGRAYGL), bound to the same Ag-presenting molecule, HLA-B8. We found that the three TCRs exhibit differing fine specificities for the viral Ag. Further, via structural and biophysical approaches, we demonstrated that the viral Ag provides the greatest energetic contribution to the TCR-peptide-HLA interaction, while focusing on a few adjacent HLA-based interactions to further tune fine-specificity requirements. Thus, the TCR engages the peptide-HLA with the viral Ag as the main glue, such that neighboring TCR-MHC interactions are recruited as a supportive adhesive. Collectively, we provide a portrait of how the host's adaptive immune response differentially engages a common viral Ag. 相似文献