全文获取类型
收费全文 | 982篇 |
免费 | 83篇 |
出版年
2022年 | 7篇 |
2021年 | 22篇 |
2020年 | 7篇 |
2019年 | 20篇 |
2018年 | 9篇 |
2017年 | 14篇 |
2016年 | 23篇 |
2015年 | 29篇 |
2014年 | 25篇 |
2013年 | 40篇 |
2012年 | 56篇 |
2011年 | 41篇 |
2010年 | 30篇 |
2009年 | 40篇 |
2008年 | 48篇 |
2007年 | 54篇 |
2006年 | 48篇 |
2005年 | 48篇 |
2004年 | 23篇 |
2003年 | 32篇 |
2002年 | 28篇 |
2001年 | 26篇 |
2000年 | 19篇 |
1999年 | 12篇 |
1998年 | 10篇 |
1997年 | 8篇 |
1996年 | 9篇 |
1994年 | 8篇 |
1992年 | 19篇 |
1991年 | 18篇 |
1990年 | 10篇 |
1989年 | 13篇 |
1988年 | 6篇 |
1987年 | 11篇 |
1986年 | 11篇 |
1985年 | 9篇 |
1984年 | 9篇 |
1981年 | 13篇 |
1980年 | 15篇 |
1979年 | 15篇 |
1978年 | 9篇 |
1976年 | 7篇 |
1975年 | 7篇 |
1974年 | 5篇 |
1973年 | 6篇 |
1970年 | 6篇 |
1969年 | 5篇 |
1968年 | 5篇 |
1962年 | 7篇 |
1961年 | 9篇 |
排序方式: 共有1065条查询结果,搜索用时 15 毫秒
141.
142.
143.
144.
Youngjun Mo Leonardo S. Vanzetti Iago Hale Emiliano J. Spagnolo Fabio Guidobaldi Jassim Al-Oboudi Natalie Odle Stephen Pearce Marcelo Helguera Jorge Dubcovsky 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(10):2021-2035
Key message
This study identified Rht25, a new plant height locus on wheat chromosome arm 6AS, and characterized its pleiotropic effects on important agronomic traits.Abstract
Understanding genes regulating wheat plant height is important to optimize harvest index and maximize grain yield. In modern wheat varieties grown under high-input conditions, the gibberellin-insensitive semi-dwarfing alleles Rht-B1b and Rht-D1b have been used extensively to confer lodging tolerance and improve harvest index. However, negative pleiotropic effects of these alleles (e.g., poor seedling emergence and reduced biomass) can cause yield losses in hot and dry environments. As part of current efforts to diversify the dwarfing alleles used in wheat breeding, we identified a quantitative trait locus (QHt.ucw-6AS) affecting plant height in the proximal region of chromosome arm 6AS (<?0.4 cM from the centromere). Using a large segregating population (~?2800 gametes) and extensive progeny tests (70–93 plants per recombinant family), we mapped QHt.ucw-6AS as a Mendelian locus to a 0.2 cM interval (144.0–148.3 Mb, IWGSC Ref Seq v1.0) and show that it is different from Rht18. QHt.ucw-6AS is officially designated as Rht25, with Rht25a representing the height-increasing allele and Rht25b the dwarfing allele. The average dwarfing effect of Rht25b was found to be approximately half of the effect observed for Rht-B1b and Rht-D1b, and the effect is greater in the presence of the height-increasing Rht-B1a and Rht-D1a alleles than in the presence of the dwarfing alleles. Rht25b is gibberellin-sensitive and shows significant pleiotropic effects on coleoptile length, heading date, spike length, spikelet number, spikelet density, and grain weight. Rht25 represents a new alternative dwarfing locus that should be evaluated for its potential to improve wheat yield in different environments.145.
Michael D. Sadick Amy Galloway David Shelton Victoria Hale Suzanne Weck Vince Anicetti Wai Lee T. Wong 《Experimental cell research》1997,234(2):354
A rapid, sensitive, and high-capacity assay has been developed to quantify ligand-induced receptor tyrosine kinase activation in terms of receptor phosphorylation. The assay, termed a “kinase receptor activation” or KIRA-ELISA, utilizes two separate microtiter plates, one for cell culture and ligand stimulation, and the other for receptor capture and phosphotyrosine ELISA. The assay was developed for analysis of neurotrophin-induced trkA, trkB, or trkC activation. It utilizes a trkA, trkB, or trkC receptor fused with a 26-amino-acid polypeptide flag derived from HSV glycoprotein D (gD.trkA, B, or C, respectively) on the amino-terminus, stably transfected into CHO cells. Stimulated receptors were solubilized with Triton X-100 buffer and then captured in ELISA wells coated with gD-specific mAb. The degree of receptor autophosphorylation was quantified by anti-phosphotyrosine ELISA. Reproducible standard curves were generated with an EC50of approximately 16 ng/ml NGF for gD.trkA KIRA, 11 ng/ml for NT4/5 and 20 ng/ml for BDNF in gD.trkB KIRA, and 9.4 ng/ml for NT3 in gD.trkC KIRA. When the gD.trkA KIRA assay was used to quantify serum NGF or NT3 following administration to rats, the assay agreed well with currently existing ELISA assays. When the gD.trkA KIRA assay was used to test several NGF variants, as well as NGF stability samples, the capacity of the assay to quantify ligand bioactivity compared well with the more widely used radioreceptor binding and PC 12 cell survival assays. The gD.trk KIRA assays show great potential as rapid bioassays, capable of quantitative, consistent, and stability indicating analyses. 相似文献
146.
Effect of Low Levels of Calcium on Exudation of Sugars and Sugar Derivatives from Intact Peanut Roots under Axenic Conditions 总被引:3,自引:1,他引:2
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The effects of 10, 20, 35 and 50 mg of Ca2+ per liter on the qualitative and quantitative exudation of sugars from roots of 5-week-old peanut plants, Arachis hypogaea L., grown axenically in nutrient solutions, were measured. Nutrient solutions in which plants had been growing were collected at weekly intervals for 4 weeks, sugars in them were measured by gasliquid chromatography of the trimethylsilyl derivatives. Arabinose, ribose, xylose, fructose, mannose, glucose, galactose, mannitol, galacturonic acid, inositol, sucrose, and five unknowns were found. Qualitative and quantitative differences in exudates were correlated with age of the plants and calcium level. Four times more sugar was exuded at 10 mg than at 50 mg of Ca2+ per liter but no significant differences in growth were observed. Ion efflux measurements suggested that low levels of Ca2+ increased root cell membrane permeability. 相似文献
147.
148.
149.
150.