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51.
Genji Matsuda Tetsuo Maita Takayuki Miyanishi Masaki Hayashida 《Journal of Protein Chemistry》1987,6(1):33-46
We have studied the primary structures of myosins from chicken muscles in order to clarify the relationship between structure and function of muscle myosin. The primary structures of the various kinds of light chains from chicken muscle myosins have been determined. We also report the primary structure of the 23K fragment of subfragment-1 (S-1) component from the heavy chain of chicken fast skeletal muscle myosin. In addition, antibody was prepared against the 23K fragment. The antibody was found to inhibit the Mg2+-ATPase activity and the initial Pi burst of the ATPase in the S-1 component. The antibody suppressed the ATP-induced fluorescence enhancement of S-1, though it did not suppress the binding of ATP to S-1. These results are also discussed.This article was presented during the proceedings of the International Conference on Macromolecular Structure and Function, held at the National Defence Medical College, Tokorozawa, Japan, December 1985. 相似文献
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Expression of DnaJ homologs and Hsp70 in the Japanese willow (Salix gilgiana Seemen). 总被引:3,自引:0,他引:3
We isolated three cDNA clones (pSGJ1, pSGJ2 and pSGJ3) for DnaJ-like proteins of the Japanese willow (Salix gilgiana Seemen). The insert in pSGJ2 included the entire nucleotide sequence of the insert in pSGJ1 that was separated into four parts by three intron-like sequences, and it might correspond to the cDNA for a precursor to the SGJ1 mRNA. The predicted amino acid sequences of SGJ1 (47.5 kDa) and SGJ3 (46.6 kDa) exhibited about 70 to 91% homology to plant cytoplasmic DnaJ-like proteins. SGJ1 was encoded by one or a few genes within the willow genome while SGJ3 was encoded by several genes. Expression of the SGJ1 and SGJ3 genes was compared with that of Hsp70 (SGK1) gene. Constitutive expression of SGJ1, SGJ3 and SGK1 was detected in all organs examined. The SGJ1 mRNA was most abundant in male catkins (inflorescences). High-level expression of SGJ3 and SGK1 was detected in catkins and roots of both male and female plants. The level of the SGJ3 mRNA in the leaves increased about 10-fold after 1 h of heat stress while the expression of SGJ1 mRNA reached a maximum level after 6 h. However, the extent of induction of SGJ1 mRNA was quite low. The levels of SGJ1 and SGJ3 mRNAs in the leaves increased about 6-fold and 2-fold, respectively, after 24 h of treatment with 100 mM NaCl. Thus, the SGJ1 and SGJ3 genes showed differential expression patterns in a variety of organs and in response to different environmental conditions. 相似文献
55.
Yoshikazu Watanabe Kohei Hayashida Daisuke Saito Toshihiro Takahashi Junichi Sakai Eriko Nakata Takashi Kanda Takashi Iwai Shigeto Hirayama Hideaki Fujii Tomio Yamakawa Hiroshi Nagase 《Bioorganic & medicinal chemistry letters》2017,27(15):3495-3498
We designed and synthesized novel δ opioid receptor (DOR) agonists 3a–i with an azatricyclodecane skeleton, which was a novel structural class of DOR agonists. Among them, 3b exhibited high values of binding affinity and potent agonistic activity for the DOR that were approximately equivalent to those of 2 which bore an oxazatricyclodecane skeleton. In vitro assays using the blood-brain barrier (BBB) permeability test kit supported the idea that 3b achieved an excellent BBB permeability by converting an oxygen atom of 2 to a carbon atom (methylene group) in the core skeleton. As a result, 3b showed potent antinociceptive effects. 相似文献
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In previous experimental studies on the visual processing in vertebrates, higher-order visual functions such as the object
segregation from background were found even in the retinal stage. Previously, the “linear–nonlinear” (LN) cascade models have
been applied to the retinal circuit, and succeeded to describe the input-output dynamics for certain parts of the circuit,
e.g., the receptive field of the outer retinal neurons. And recently, some abstract models composed of LN cascades as the
circuit elements could explain the higher-order retinal functions. However, in such a model, each class of retinal neurons
is mostly omitted and thus, how those neurons play roles in the visual computations cannot be explored. Here, we present a
spatio-temporal computational model of the vertebrate retina, based on the response function for each class of retinal neurons
and on the anatomical inter-cellular connections. This model was capable of not only reproducing the spatio-temporal filtering
properties of the outer retinal neurons, but also realizing the object segregation mechanism in the inner retinal circuit
involving the “wide-field” amacrine cells. Moreover, the first-order Wiener kernels calculated for the neurons in our model
showed a reasonable fit to the kernels previously measured in the real retinal neuron in situ. 相似文献
59.
Background: For understanding biological cellular systems, it is important to analyze interactions between protein residues and RNA bases. A method based on conditional random fields (CRFs) was developed for predicting contacts between residues and bases, which receives multiple sequence alignments for given protein and RNA sequences, respectively, and learns the model with many parameters involved in relationships between neighboring residue-base pairs by maximizing the pseudo likelihood function. Methods: In this paper, we proposed a novel CRF-based model with more complicated dependency relationships between random variables than the previous model, but which takes less parameters for the sake of avoidance of overfitting to training data. Results: We performed cross-validation experiments for evaluating the proposed model, and took the average of AUC (area under receiver operating characteristic curve) scores. The result suggests that the proposed CRF-based model without using L1-norm regularization (lasso) outperforms the existing model with and without the lasso under several input observations to CRFs. Conclusions: We proposed a novel stochastic model for predicting protein-RNA residue-base contacts, and improved the prediction accuracy in terms of the AUC score. It implies that more dependency relationships in a CRF could be controlled by less parameters. 相似文献
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Psychological effects of Helicobacter pylori‐associated atrophic gastritis in patients under 50 years: A cross‐sectional study
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