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991.
Habitat losses occur non-randomly within human-modified landscapes, resulting in high spatial heterogeneity of local habitat histories. Although local habitat history can modulate the existence of extinction debt (i.e., the number of populations predicted to become extinct) in a landscape, its role in detecting extinction debt has not been examined explicitly. We aimed to compare the detectability of extinction debt among populations of an endangered semi-natural grassland species, Echinops setifer (Compositae), in the grassland landscape of Mt. Aso, Japan. We classified populations into three groups that differed in local habitat history: stable (habitat loss ≤30% since the 1930s), moderately decreased (30% < loss ≤ 90%), and severely decreased (loss >90%). We then evaluated whether the effects of habitat areas during the 1930s and 2000s varied among groups to explain population size by GLMMs and estimated coefficient of explanatory variable by Bayesian MCMC methods. Within the groups, stable group showed significant positive relationships with both past and current habitat areas. The moderately decreased group only showed significant positive relationships with past habitat areas, indicating the existence of extinction debt in these populations. The severely decreased group only showed significant positive relationships with current habitat areas, indicating that they may have already paid their extinction debt because the rate of grassland loss exceeded the extinction threshold. Even within the same landscape, extinction debt varied in response to local habitat history. In spatially heterogeneous landscapes, evaluation of effects of local habitat history can elucidate the habitat-based extinction risks for plant populations. 相似文献
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993.
Yasuharu Abe Aya Nambu Sachiko Yamaguchi Ayako Takamori Hajime Suto Sachiko Hirose Tadashi Yokosuka Susumu Nakae Katsuko Sudo 《Biochemistry and Biophysics Reports》2017
Interleukin (IL)-25, which is a member of the IL-17 family of cytokines, induces production of such Th2 cytokines as IL-4, IL-5, IL-9 and/or IL-13 by various types of cells, including Th2 cells, Th9 cells and group 2 innate lymphoid cells (ILC2). On the other hand, IL-25 can suppress Th1- and Th17-associated immune responses by enhancing Th2-type immune responses. Supporting this, IL-25 is known to suppress development of experimental autoimmune encephalitis, which is an IL-17-mediated autoimmune disease in mice. However, the role of IL-25 in development of IL-17-mediated arthritis is not fully understood. Therefore, we investigated this using IL-1 receptor antagonist-deficient (IL-1Ra-/-) mice, which spontaneously develop IL-17-dependent arthritis. However, development of spontaneous arthritis (incidence rate, disease severity, proliferation of synovial cells, infiltration of PMNs, and bone erosion in joints) and differentiation of Th17 cells in draining lymph nodes in IL-25-/- IL-1Ra-/- mice were similar to in control IL-25+/+ IL-1Ra-/- mice. These observations indicate that IL-25 does not exert any inhibitory and/or pathogenic effect on development of IL-17-mediated spontaneous arthritis in IL-1Ra-/- mice. 相似文献
994.
Maki Nemoto Takashi Kuda Mika Eda Hiroshi Yamakawa Hajime Takahashi Bon Kimura 《Probiotics and antimicrobial proteins》2017,9(1):48-55
Most wakame Undaria pinnatifida, a brown algae, products are made from the frond portion. In this study, the polysaccharide content and antioxidant property of aqueous extract solutions (AESs) of the four parts (frond: wakame, stem of the frond: kuki-wakame, sporophyll: mekabu, and kuki-mekabu) of wakame were investigated. Polysaccharide content was high in both the wakame and mekabu. Superoxide anion (O2 ?) radical-scavenging capacities were high in the mekabu. These AESs could be fermented by Lactobacillus plantarum Sanriku-SU7. The O2 ? radical-scavenging activity of the kuki-wakame, mekabu, and kuki-mekabu were increased by the fermentation. Fermented mekabu clearly showed a protective effect on human enterocyte-like HT-29-luc cells and in a mouse model of dextran sodium sulphate-induced inflammatory bowel disease (IBD). These results suggest that the mekabu fermented by L. plantarum Sanriku-SU7 has anti-IBD effect related to O2 ? radical-scavenging. 相似文献
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997.
Characterization of a novel allergenic protein from the octocoral Scleronephthya gracillima (Kuekenthal) that corresponds to a new GFP‐like family named Akane 下载免费PDF全文
Yuko Kato Mitsuru Jimbo Youichi Sakakibara Reiko Onizuka Tatsuya Takahashi Sachiko Matsuhashi Hajime Mita Kei Amada Yukimitsu Imahara Kimiko Tanabe Akihisa Toda Hisao Kamiya 《Luminescence》2017,32(6):1009-1016
Certain marine organisms have been known to cause allergic reactions among occupational fishermen. We have previously reported that bronchial asthma among the workers engaged in spiny lobster fishing in Japan was caused by octocorals such as Dendronephthya sp. and Scleronephthya gracillima (previously named Alcyonium gracillimum). Now we have found another octocoral, Scleronephthya gracillima (Kuekenthal), which causes the allergic disease in fishermen. The octocoral was characterized as a new green fluorescent protein (GFP)‐like family. The new allergen has a molecular mass of 27 kDa in 1D and 2D SDS‐PAGE under reduced conditions. The 27 kDa component was determined to be an allergen by western blotting, ECL immune staining method and absorption of patient sera with the antigen. Furthermore, the combination of analysis with LC‐ESI‐MS/MS and MASCOT search in the NCBInr database concluded the 27 kDa component had the sequence YPADI/LPDYFK, and that the 22 kDa component had the sequence QSFPEGFSWER, which both matched a GFP‐like protein in Acropora aculeus and in Montastraea annularis. Further analysis by MALDI‐TOF/MS/MS and MASCOT search in the NCBInr database of all 27 kDa eight spot components from 2D SDS‐PAGE indicated that the sequence QSFPEGFSWER also matched as GFP‐like protein in Lobophyllia hemprichii and Scleractinia sp. To our knowledge, this is the first report of the new allergenic protein that corresponds to a new GFP‐like protein named Akane, and which has fluorescent emissions in the red and green part of the spectra at 628 nm and 508 nm, respectively. 相似文献
998.
Soichi Kugimiya Ritsuo Nishida Yasumasa Kuwahara Masayuki Sakuma 《Entomologia Experimentalis et Applicata》2002,104(2-3):337-344
As a part of the sequential courtship behavior of the German cockroach, Blattella germanica, females feed on the nuptial secretion from the male tergal glands. The pheromonal secretion, consisting mainly of oligosaccharides and phospholipids, strongly elicits a feeding response in virgin females. The phospholipids were composed of phosphatidylethanolamine and phosphatidylcholine. Their fatty acid compositions were determined by chromatographic and enzymatic methods. Although an authentic blend of phospholipids (1,2-dioleoylphosphatidylethanolamine and 1,2-dioleoylphosphatidylcholine) showed a marginal phagostimulant activity, addition of the blend to an authentic blend of oligosaccharides (maltose and maltotriose) strongly enhanced the activity to nearly the level of the crude extract. These results indicate that the nuptial feeding behavior is elicited by a synergistic action between phospholipids and oligosaccharides. 相似文献
999.
Noboru Sato Chie Sakuma Hiromi Kato Carolanne E. Milligan Ronald W. Oppenheim Hiroyuki Yaginuma 《Developmental neurobiology》2002,53(3):381-390
Motoneurons (MNs) in the cervical spinal cord of the chicken embryo undergo programmed cell death (PCD) between embryonic day (E) 4 and E5. The intracellular molecules regulating this early phase of PCD remain unknown. Here we show that introduction of Bcl‐2 by a replication‐competent avian retroviral vector prevented MN degeneration at E4.5, whereas the expression of the green fluorescent protein (GFP) was ineffective. Bcl‐2 expression did not affect the number of Islet‐1/2‐positive MNs at the onset of cell death (E4). However, when examined at the end of the cell death period (E5.5), the number of Islet‐1/2‐positive MNs was clearly increased in Bcl‐2‐transfected embryos compared with control and GFP‐transfected embryos. Activation of caspase‐3, which is normally observed in this early MN death, was also prevented by Bcl‐2. Thus, MNs in the cervical spinal cord appear to use intracellular pathway(s) for early PCD that is responsive to Bcl‐2. © 2002 Wiley Periodicals, Inc. J Neurobiol 53: 381–390, 2002 相似文献
1000.
Hajime Hirasawa Richard Shiells Masahiro Yamada 《The Journal of general physiology》2002,119(1):55-68
The role of group III metabotropic glutamate receptors (mGluRs) in photoreceptor-H1 horizontal cell (HC) synaptic transmission was investigated by analyzing the rate of occurrence and amplitude of spontaneous excitatory postsynaptic currents (sEPSCs) in H1 HCs uncoupled by dopamine in carp retinal slices. Red light steps or the application of 100 microM cobalt reduced the sEPSC rate without affecting their peak amplitude, which is consistent with hyperpolarization or the suppression of Ca(2+) entry into cone synaptic terminals reducing vesicular transmitter release. Conversely, postsynaptic blockade of H1 HC AMPA receptors by 500 nM CNQX reduced the amplitude of sEPSCs without affecting their rate. This analysis of sEPSCs represents a novel methodology for distinguishing between presynaptic and postsynaptic sites of action. The selective agonist for group III mGluRs, l-2-amino-4-phosphonobutyrate (L-APB or L-AP4; 20 microM), reduced the sEPSC rate with a slight reduction in amplitude, which is consistent with a presynaptic action on cone synaptic terminals to reduce transmitter release. During L-APB application, recovery of sEPSC rate occurred with 500 microM (s)-2-methyl-2-amino-4-phosphonobutyrate (MAP4), a selective antagonist of group III mGluR, and with 200 microM 4-aminopyridine (4-AP), a blocker of voltage-dependent potassium channels. Whole-cell recordings from cones in the retinal slice showed no effect of L-APB on voltage-activated Ca(2+) conductance. These results suggest that the activation of group III mGluRs suppresses transmitter release from cone presynaptic terminals via a 4-AP-sensitive pathway. Negative feedback, operating via mGluR autoreceptors, may limit excessive glutamate release from cone synaptic terminals. 相似文献