全文获取类型
收费全文 | 3736篇 |
免费 | 235篇 |
出版年
2021年 | 34篇 |
2020年 | 19篇 |
2019年 | 39篇 |
2018年 | 39篇 |
2017年 | 45篇 |
2016年 | 53篇 |
2015年 | 82篇 |
2014年 | 92篇 |
2013年 | 309篇 |
2012年 | 179篇 |
2011年 | 169篇 |
2010年 | 104篇 |
2009年 | 105篇 |
2008年 | 199篇 |
2007年 | 222篇 |
2006年 | 213篇 |
2005年 | 189篇 |
2004年 | 223篇 |
2003年 | 213篇 |
2002年 | 205篇 |
2001年 | 96篇 |
2000年 | 92篇 |
1999年 | 80篇 |
1998年 | 69篇 |
1997年 | 57篇 |
1996年 | 44篇 |
1995年 | 52篇 |
1994年 | 38篇 |
1993年 | 39篇 |
1992年 | 61篇 |
1991年 | 68篇 |
1990年 | 43篇 |
1989年 | 55篇 |
1988年 | 36篇 |
1987年 | 34篇 |
1986年 | 20篇 |
1985年 | 29篇 |
1984年 | 35篇 |
1983年 | 27篇 |
1982年 | 22篇 |
1981年 | 18篇 |
1980年 | 18篇 |
1979年 | 21篇 |
1978年 | 18篇 |
1977年 | 19篇 |
1976年 | 20篇 |
1975年 | 18篇 |
1973年 | 15篇 |
1971年 | 17篇 |
1969年 | 11篇 |
排序方式: 共有3971条查询结果,搜索用时 15 毫秒
61.
Hidenori Sassa Takeshi Nishio Yasuo Kowyama Hisashi Hirano Takato Koba Hiroshi Ikehashi 《Molecular & general genetics : MGG》1996,250(5):547-557
Stylar riboncleases (RNases) are associated with gametophytic self-incompatibility in two plant families, the Solanaceae and the Rosaceae. The self-incompatibility-associated RNases (S-RNases) of both the Solanaceae and the Rosaceae were recently reported to belong to the T2 RNase gene family, based on the presence of two well-conserved sequence motifs. Here, the cloning and characterization of S-RNase genes from two species of Rosaceae, apple (Malus × domestica) and Japanese pear (Pyrus serotina) is described and these sequences are compared with those of other T2-type RNases. The S-RNases of apple specifically accumulated in styles following maturation of the flower bud. Two cDNA clones for S-RNases from apple, and PCR clones encoding a further two apple S-RNases as well as two Japanese pear S-RNases were isolated and sequenced. The deduced amino acid sequences of the rosaceous S-RNases contained two conserved regions characteristic of the T2/S-type RNases. The sequences showed a high degree of diversity, with similarities ranging from 60.4% to 69.2%. Interestingly, some interspecific sequence similarities were higher than those within a species, possibly indicating that diversification of S-RNase alleles predated speciation in the Rosaceae. A phylogenetic tree of members of the T2/S-RNase superfamily in plants was obtained. The rosaceous S-RNases formed a new lineage in the tree that was distinct from those of the solanaceous S-RNases and the S-like RNases. The findings suggested that self-incompatibility mechanisms in Rosaceae and Solanaceae are similar but arose independently in the course of evolution. 相似文献
62.
C. Schönbach Kiyoshi Miwa Masaaki Ibe Hajime Shiga Kiyoshi Nokihara Masafumi Takiguchi 《Immunogenetics》1996,45(2):121-129
HLA-B*3501 is associated with subacute thyroiditis and fast progression of AIDS. An important prerequisite to investigate the T-cell
recognition of HLA-B*3501-restricted antigens is the characterization of peptide-HLA-B*3501 interactions. In this study, peptide-HLA-B*3501 interactions were determined in quantitative peptide binding assays. The results were statistically analyzed to evaluate
the influence of both anchor and nonanchor positions and the predictability of peptide binding. The binding data demonstrated
that all anchor residues at position 2 and the C-terminus found in 9-mers functioned equally as anchors in 10-mers and 11-mers.
These minimum requirements of peptide binding were refined by assessing positive and negative effects of nonanchor residues.
Aliphatic hydrophobic residues at positions 3, 5, and 8 of 10-mers and position 3 of 11-mers significantly enhanced HLA-B*3501 binding. Similar effects rendered aromatic, bulky residues, acidic or polar residues of 11-mers at position 1 as well
as at positions 4, 8, and 10, respectively. Negative effects were observed for residues carrying positively charged side-chains
at position 7 of 11-mers. The refined HLA-B*3501 peptide binding motifs enhanced the identification of potential T-cell epitopes. The disparity between positive effects
at the middle and C-terminal part (positions 5 – 8 and 10) of 11-mers and shorter peptides supports the extrusion of 11-mer
residues at positions 5, 6, and 7, away from the HLA-B*3501 binding cleft.
Received: 29 May 1996 / Revised: 5 August 1996 相似文献
63.
64.
Myosin molecules contacting an actin filament in the presence of ATP were found to regulate the filamental fluctuations due to ATP hydrolysis in a communicative manner along the filament. As an evidence of the occurrence of the communication, ATP-activated fluctuating displacements of the filament in the direction perpendicular to its longitudinal axis were identified to propagate at a finite velocity not less than about 0.2 μm/s unidirectionally along the filament. 相似文献
65.
Raindrop Momentum Triggers Growth of Leaf-Associated Populations of Pseudomonas syringae on Field-Grown Snap Bean Plants
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Observational and microclimate modification experiments were conducted under field conditions to determine the role of the physical environment in effecting large increases in phyllosphere population sizes of Pseudomonas syringae pv. syringae, the causal agent of bacterial brown spot disease of snap bean (Phaseolus vulgaris L.). Comparisons of daily changes in population sizes of P. syringae on three plantings of snap bean cultivar Cascade and one of cultivar Eagle with weather conditions indicated a strong association of rainfalls with periods of 1 to 3 days in duration during which increases in bacterial population sizes were greater than 10-fold and up to 1,000-fold. The effects of rain on populations of P. syringae were explored further by modifying the microclimate of bean plants in the field with polyethylene shelters to shield plants from rain and fine-mesh inert screens to modify the momentum of raindrops. After each of three separate intense rains, the greater-than-10-fold increases in population sizes of P. syringae observed on plants exposed to the rains did not occur on plants in the shelters or under the screens. The screens decreased the velocity and, thus, the momentum of raindrops but not the volume or quality of rainwater that fell on plants under the screens. Thus, the absence of increases in population sizes of P. syringae on plants under the screens suggests that raindrop momentum plays a role in the growth-triggering effect of intense rains on populations of P. syringae on bean plants under field conditions. 相似文献
66.
67.
68.
The interaction of small domains between the subunits of phosphatidylinositol 3-kinase determines enzyme activity. 总被引:13,自引:7,他引:6
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Previous studies have suggested that the two subunits of phosphatidylinositol (PI) 3-kinase, p85 and p110, function as localizing and catalytic subunits, respectively. Using recombinant p85 and p110 molecules, we have reconstituted the specific interaction between the two subunits of mouse PI 3-kinase in cells and in vitro. We have previously shown that the region between the two Src homology 2 (SH2) domains of p85 is able to form a functional complex with the 110-kDa subunit in vivo. In this report, we identify the corresponding domain in p110 which directs the binding to p85. We demonstrate that the interactive domains in p85 and p110 are less than 103 and 124 amino acids, respectively, in size. We also show that the association of p85 and p110 mediated by these domains is critical for PI 3-kinase activity. Surprisingly, a complex between a 102-amino-acid segment of p85 and the full-length p110 molecule is catalytically active, whereas p110 alone has no activity. In addition to the catalytic domain in the carboxy-terminal region, 123 amino acids at the amino terminus of p110 were required for catalytic activity and were sufficient for the interaction with p85. These results indicate that the 85-kDa subunit, previously thought to have only a linking role in localizing the p110 catalytic subunit, is an important component of the catalytic complex. 相似文献
69.
Shinichi Kitamura Takashi Hirano Kenichi Takeo Mitsuru Mimura Kanji Kajiwara Bjrn T. Stokke Tsutomu Harada 《International journal of biological macromolecules》1994,16(6)
The conformation and dilute solution properties of (2→1)-β-d-fructan in aqueous solution were studied by gel permeation chromatography, low-angle laser light-scattering photometry, viscometry, small-angle X-ray scattering and electron microscopy. Fractions covering a broad range of weight-average molecular weights (Mw) from 1.49 × 104 to 5.29 × 106 were obtained from a native sample by ultrasonic degradation and fractional precipitation. For Mw < 4 × 104, the intrinsic viscosity [η] varies with Mw0.71, indicating that the fructan chain behaves as a random coil expanded by an excluded-volume effect in this molecular weight region. For Mw > 105, [η] exhibits an unusually weak dependence on Mw and finally becomes almost independent of molecular weight. This behaviour is interpreted in terms of a globular conformation of the high-molecular-weight fructan molecules. Small-angle X-ray-scattering measurements and electron microscopic observations support this interpretation of the values of [η] observed. 相似文献
70.
Toshio Sakamoto Hajime Wada Ikuo Nishida Masayuki Ohmori Norio Murata 《Plant molecular biology》1994,24(4):643-650
Cyanobacterial genes for enzymes that desaturate fatty acids at the 12 position, designated desA, were isolated from Synechocystis PCC6714, Synechococcus PCC7002 and Anabaena variabilis by crosshybridization with a DNA probe derived from the desA gene of Synechocystis PCC6803. The genes of Synechocystis PCC6714, Synechococcus PCC7002 and A. variabilis encode proteins of 349, 347 and 350 amino acid residues, respectively. The transformation of Synechococcus PCC7942 with the desA genes from Synechocystis PCC6714, Synechococcus PCC7002 and A. variabilis was associated with the ability to introduce a second double bond at the 12 position of fatty acids. The amino acid sequence of the products of the desA genes revealed the presence of four conserved domains. Since one of the conserved domains was also found in the amino acid sequences of 3 desaturases of Brassica napus and mung bean, this domain may play an essential role in the introduction of a double bond into fatty acids bound to membrane lipids.Abbreviations X:Y(Z)
fatty acid containing X carbon atoms with Y double bonds in the cis configuration at position Z counted from the carboxyl terminus 相似文献