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11.
Ghofrani Narges Soltani Sheikhi Maedeh Amirzakaria Javad Zamani Hassani Sorour Aminzadeh Saeed Haghbeen Kamahldin 《The protein journal》2021,40(5):689-698
The Protein Journal - As a safe substitute for hydroquinone, β-arbutin, a natural plant substance, and its synthetic counterpart, α-arbutin, are used in depigmentation formulations.... 相似文献
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Mahbobeh Zamani Babgohari Ali Niazi Ali Asghar Moghadam Tahereh Deihimi Esmaeil Ebrahimie 《In vitro cellular & developmental biology. Plant》2013,49(2):97-106
Exclusion of sodium ions from cells is one of the key salinity tolerance mechanisms in plants. The high-affinity cation transporter (HKT1;5) is located in the plasma membrane of the xylem, excluding Na+ from the parenchyma cells to reduce Na+ concentration. The regulatory mechanism and exact functions of HKT genes from different genotypic backgrounds are relatively obscure. In this study, the expression patterns of HKT1;5 in A and D genomes of wheat were investigated in root and leaf tissues of wild and domesticated genotypes using real-time PCR. In parallel, the K+/Na+ ratio was measured in salt-tolerant and salt-sensitive cultivars. Promoter analysis were applied to shed light on underlying regulatory mechanism of the HKT1;5 expression. Gene isolation and qPCR confirmed the expression of HKT1;5 in the A and D genomes of wheat ancestors (Triticum boeoticum, AbAb and Aegilops crassa, MMDD, respectively). Interestingly, earlier expression of HKT1;5 was detected in leaves compared with roots in response to salt stress. In addition, the salt-tolerant genotypes expressed HKT1;5 before salt-sensitive genotypes. Our results suggest that HKT1;5 expression follows a tissue- and genotype-specific pattern. The highest level of HKT1;5 expression was observed in the leaves of Aegilops, 6 h after being subjected to high salt stress (200 mM). Overall, the D genome allele (HKT1;5-D) showed higher expression than the A genome (HKT1;5-A) allele when subjected to a high NaCl level. We suggest that the D genome is more effective regarding Na+ exclusion. Furthermore, in silico promoter analysis showed that TaHKT1;5 genes harbor jasmonic acid response elements. 相似文献
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Ali Asghar Moghadam Eemaeil Ebrahimie Seyed Mohsen Taghavi Ali Niazi Mahbobeh Zamani Babgohari Tahereh Deihimi Mohammad Djavaheri Amin Ramezani 《Molecular biotechnology》2013,54(3):756-769
A small number of stress-responsive genes, such as those of the mitochondrial F1F0-ATP synthase complex, are encoded by both the nucleus and mitochondria. The regulatory mechanism of these joint products is mysterious. The expression of 6-kDa subunit (MtATP6), a relatively uncharacterized nucleus-encoded subunit of F0 part, was measured during salinity stress in salt-tolerant and salt-sensitive cultivated wheat genotypes, as well as in the wild wheat genotypes, Triticum and Aegilops using qRT-PCR. The MtATP6 expression was suddenly induced 3 h after NaCl treatment in all genotypes, indicating an early inducible stress-responsive behavior. Promoter analysis showed that the MtATP6 promoter includes cis-acting elements such as ABRE, MYC, MYB, GTLs, and W-boxes, suggesting a role for this gene in abscisic acid-mediated signaling, energy metabolism, and stress response. It seems that 6-kDa subunit, as an early response gene and nuclear regulatory factor, translocates to mitochondria and completes the F1F0-ATP synthase complex to enhance ATP production and maintain ion homeostasis under stress conditions. These communications between nucleus and mitochondria are required for inducing mitochondrial responses to stress pathways. Dual targeting of 6-kDa subunit may comprise as a mean of inter-organelle communication and save energy for the cell. Interestingly, MtATP6 showed higher and longer expression in the salt-tolerant wheat and the wild genotypes compared to the salt-sensitive genotype. Apparently, salt-sensitive genotypes have lower ATP production efficiency and weaker energy management than wild genotypes; a stress tolerance mechanism that has not been transferred to cultivated genotypes. 相似文献
15.
Mina Baharloei Behrooz Heidari Hojjatolah Zamani Mahvash Hadavi 《Zeitschrift fur angewandte Ichthyologie》2020,36(4):393-401
The induction of Hsps (heat shock protein) recognized as a promising approach to limiting disease and improving health in aquaculture. This investigation aimed to study the impacts of Pro-Tex®, an extract from the prickly pear cactus (Opuntia ficus indica), on the expression of Hsp70 gene and induction of immune response parameters in Acipenser persicus infected with Aeromonas hydrophila ATCC®7966TM. Fish were pretreated with 25, 50 and 100 mg/L of Pro-Tex and then injected in the intra-peritoneal cavity with A. hydrophila. The expression level of Hsp70 gene, lysozyme activity (LYZ) and complement C3 (C3), and immunoglobulin M (IgM) levels were assessed in liver, gill, and intestine on the days 3 and 7 post-infection. Tex-OE® increased expression of Hsp70 in a dose-dependent way in A. persicus, but this expression significantly reduced on the 7-days post-injection. The Hsp70 expression pattern was variable in each tissue, also, LYZ activity, C3, and IgM increased, depending on the concentration, and showed a decreasing trend in a time-dependent way. In conclusion, our data indicated that Pro-Tex as an Hsp70 inducer increases the resistance of sturgeon fry against fish pathogens by induction of different immunity factors. 相似文献
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Mesenchymal stem cells (MSCs) are a major component of various forms of tissue engineering. MSCs have self-renewal and multidifferential potential. Osteogenic differentiation of MSCs is an area of attention in bone regeneration. One form of MSCs are adipose-derived stem cells (ASCs), which can be simply harvested and differentiated into several cell lineages, such as chondrocytes, adipocytes, or osteoblasts. Due to special properties, ASCs are frequently used in vitro and in vivo bone regeneration. Identifying factors involved in osteogenic differentiation of ASCs is important for better understanding the mechanism of osteogenic differentiation. Different methods are used to stimulate osteogenesis of ASCs in literature, including common osteogenic media, growth factors, hormones, hypoxia, mechanical and chemical stimuli, genetic modification, and nanotechnology. This review article provides an overview describing the isolation procedure, characterization, properties, current methods for osteogenic differentiation of ASCs, and their basic biological mechanism. 相似文献
17.
P. Mokarram M. Zamani S. Kavousipour F. Naghibalhossaini C. Irajie M. Moradi Sarabi S. V. Hosseini 《Molecular biology reports》2013,40(5):3851-3857
Colorectal cancer (CRC) is the third most common cancer worldwide. Colorectal cancer incidence differs widely among different geographic regions. In addition to mutational changes, epigenetic mechanisms also play important roles in the pathogenesis of CRCs. O6-methylguanine-DNA methyltransferase (O 6 -MGMT) is a DNA repair protein and in the absence of MGMT activity, G-to-A transition may accumulate in the specific genes such as K-ras and p53. To identify which CpG sites are critical for its downregulation, we analyzed the methylation status of the MGMT gene promoter in two sites in CRC patients. Then we compared the frequency of their methylation changes with the results of our previously reported K-ras gene mutation, APC2 and p16 methylation. MGMT methylation was examined in 92 tumor samples. A methylation specific PCR (MSP) method was performed for two loci of MGMT gene which described as MGMT-A and MGMT-B. The prevalence of MGMT-A, and MGMT-B methylation was 49/91 (53.8 %), and 83/92 (90.2 %), respectively. We detected high frequency of MGMT-B but not MGMT-A methylation in tumor tissues with APC2 methylation. Our results showed that MGMT-B methylation is significantly associated with K-ras gene mutation rather than MGMT-A (p = 0.04). Simultaneously, an inverse correlation was found between p16 and MGMT-B methylation simultaneously (p = 0.02). Our study indicated that hypermethylation of the specific locus near the MGMT start codon is critical for cancer progression. MGMT-B assessment that is associated with K-ras mutation can have a prognostic value in patients with CRC. 相似文献
18.
BMPR-1B and GDF9 genes are well known due to their important effects on litter size and mechanisms controlling ovulation rate in sheep. In the present study, polymorphisms of BMPR-1B gene exon 8 and GDF9 gene exon 1 were detected by single strand conformational polymorphism (SSCP) analysis and DNA sequencing methods in 100 Mehraban ewes. The PCR reaction forced to amplify 140 and 380-bp fragments of BMPR-1B and GDF9 genes, respectively. Two single nucleotide polymorphisms (SNPS) were identified in two different SSCP patterns of BMPR-1B gene (CC and CA genotypes) that deduced one amino acid exchange. Also, two SNPS were identified in three different SSCP patterns of GDF9 gene (AA, AG and GG genotypes) that deduced one amino acid exchanges. Two different secondary structures of protein were predicted for BMPR-1B exon 8, but the secondary protein structures predicted for GDF9 exon 1 were similar together. The evaluation of the associations between the SSCP patterns and the protein structure changes with reproduction traits showed that BMPR-1B exon 8 genotypes have significant effects on some of reproduction traits but the GDF9 genotypes did not have any significant effect. The CA genotype of BMPR-1B exon 8 had a significant positive effect on reproduction performance and could be considered as an important and new mutation, affecting the ewes reproduction performance. Marker assisted selection using BMPR-IB gene could be noticed to improve the reproduction traits in Mehraban sheep. 相似文献
19.
Polymeroid trilobite fauna from two stratigraphic sections (Godbondar and Kuhbanan) of the Kuhbanan Formation in northern Kerman (central Iran) were studied and subjected to biostratigraphic analysis. Eleven genera and species are recognised from the latest Early Cambrian and Middle Cambrian Peri-Gondwanian successions of the study sections. The recognised fauna includes Afghanocare lategenatum, Blountia blountia, Iranoleesia sp., Iranoleesia pisiformis, Kermanella kuhbananensis, Kermanella lata lata, Kermanella lata minuta, Kermanella sp., Redlichia chinensis, Redlichia noetlingi and Redlichia sp. Based on trilobite distribution, three trilobite biozones were recognised in the study sections, namely Redlichia noetlingi biozone, Kermanella kuhbananensis biozone and Iranoleesia pisiformis biozone. The age of the study sections is late Early Cambrian to late Middle Cambrian based on the recognised trilobite biozones. The recognised late Early Cambrian trilobite assemblages (especially Redlichia and Kermanella) from northern Kerman are similar to those found from some other parts of Gondwana or Peri-Gondwana terrains (north India, Pakistan, northwestern Kashmir, Tajikistan, South Australia, South China and Afghanistan) and show affinities with fauna found in some other parts of Iran (Alborz, northern Iran; Tabas, eastern Iran; southeast Karman). 相似文献
20.
Badr Benjelloun Frdric Boyer Ian Streeter Wahid Zamani Stefan Engelen Adriana Alberti Florian J. Alberto Mohamed BenBati Mustapha Ibnelbachyr Mouad Chentouf Abdelmajid Bechchari Hamid R. Rezaei Saeid Naderi Alessandra Stella Abdelkader Chikhi Laura Clarke James Kijas Paul Flicek Pierre Taberlet Franois Pompanon 《Molecular ecology resources》2019,19(6):1497-1515
Whole genome sequences (WGS) greatly increase our ability to precisely infer population genetic parameters, demographic processes, and selection signatures. However, WGS may still be not affordable for a representative number of individuals/populations. In this context, our goal was to assess the efficiency of several SNP genotyping strategies by testing their ability to accurately estimate parameters describing neutral diversity and to detect signatures of selection. We analysed 110 WGS at 12× coverage for four different species, i.e., sheep, goats and their wild counterparts. From these data we generated 946 data sets corresponding to random panels of 1K to 5M variants, commercial SNP chips and exome capture, for sample sizes of five to 48 individuals. We also extracted low‐coverage genome resequencing of 1×, 2× and 5× by randomly subsampling reads from the 12× resequencing data. Globally, 5K to 10K random variants were enough for an accurate estimation of genome diversity. Conversely, commercial panels and exome capture displayed strong ascertainment biases. Besides the characterization of neutral diversity, the detection of the signature of selection and the accurate estimation of linkage disequilibrium (LD) required high‐density panels of at least 1M variants. Finally, genotype likelihoods increased the quality of variant calling from low coverage resequencing but proportions of incorrect genotypes remained substantial, especially for heterozygote sites. Whole genome resequencing coverage of at least 5× appeared to be necessary for accurate assessment of genomic variations. These results have implications for studies seeking to deploy low‐density SNP collections or genome scans across genetically diverse populations/species showing similar genetic characteristics and patterns of LD decay for a wide variety of purposes. 相似文献