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Universal phenotyping techniques that can discriminate among various states of biological systems have great potential. We applied 557 fluorescent library compounds to NCI's 60 human cancer cell-lines (NCI-60) to generate a systematic fluorescence phenotypic profiling data. By the kinetic fluorescence intensity analysis, we successfully discriminated the organ origin of all the 60 cell-lines. 相似文献
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Plasmonics - Surface plasmon resonance is one of the important properties of noble metals which can be controlled by shape, size, and composition. This feature helps scientists to detect individual... 相似文献
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Identification and quantification of blood–brain barrier transporters in isolated rat brain microvessels
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Hajar Al Feteisi Zubida M. Al‐Majdoub Brahim Achour Narciso Couto Amin Rostami‐Hodjegan Jill Barber 《Journal of neurochemistry》2018,146(6):670-685
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Rosi Bissinger Elisabeth Lang Irene Gonzalez-Menendez Leticia Quintanilla-Martinez Mehrdad Ghashghaeinia Lisann Pelzl Basma Sukkar Abdulla Al Mamun Bhuyan Madhuri S. Salker Yogesh Singh Birgit Fehrenbacher Hajar Fakhri Anja T. Umbach Martin Schaller Syed M. Qadri Florian Lang 《Apoptosis : an international journal on programmed cell death》2018,23(11-12):641-650
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Lara Hajar Louis François Carla Khater Ihab Jomaa Michel Déqué Rachid Cheddadi 《Comptes rendus biologies》2010,333(8):622-630
Long-term vegetation studies are needed to better predict the impact of future climate change on vegetation structure and distribution. According to the IPCC scenario, the Mediterranean region is expected to undergo significant climatic variability over the course of this century. Cedrus libani (A. Rich), in particular, is currently distributed in limited areas in the Eastern Mediterranean region, which are expected to be affected by such climate change. In order to predict the impact of future global warming, we have used fossil pollen data and model simulations. Palaeobotanical data show that C. libani has been affected by both climate change and human activities. Populations of C. libani survived in refugial zones when climatic conditions were less favourable and its range extended during periods of more suitable climate conditions. Simulations of its future geographical distribution for the year 2100 using a dynamic vegetation model show that only three areas from Mount Lebanon may allow its survival. These results extrapolated for cedar forests for the entire Eastern Mediterranean region show that forests in Syria are also threatened by future global warming. In southern Turkey, cedar forests seem to be less threatened. These results are expected to help in the long-term conservation of cedar forests in the Near East. 相似文献
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Linkage analysis of chromosome 1q markers in 136 prostate cancer families. The Cancer Research Campaign/British Prostate Group U.K. Familial Prostate Cancer Study Collaborators. 总被引:2,自引:0,他引:2
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R A Eeles F Durocher S Edwards D Teare M Badzioch R Hamoudi S Gill P Biggs D Dearnaley A Ardern-Jones A Dowe R Shearer D L McLennan R L Norman P Ghadirian A Aprikian D Ford C Amos T M King F Labrie J Simard S A Narod D Easton W D Foulkes 《American journal of human genetics》1998,62(3):653-658
Prostate cancer shows evidence of familial aggregation, particularly at young ages at diagnosis, but the inherited basis of familial prostate cancer is poorly understood. Smith et al. recently found evidence of linkage to markers on 1q, at a locus designated "HPC1," in 91 families with multiple cases of early-onset prostate cancer. Using both parametric and nonparametric methods, we attempted to confirm this finding, in 60 affected related pairs and in 76 families with three or more cases of prostate cancer, but we found no significant evidence of linkage. The estimated proportion of linked families, under a standard autosomal dominant model, was 4%, with an upper 95% confidence limit of 31%. We conclude that the HPC1 locus is responsible for only a minority of familial prostate cancer cases and that it is likely to be most important in families with at least four cases of the disease. 相似文献
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Zelin Shan Niloofar Ghadirian Dmitry Lyumkis Nancy.C. Horton 《The Journal of biological chemistry》2022,298(4)
Enzyme filamentation is a widespread phenomenon that mediates enzyme regulation and function. For the filament-forming sequence-specific DNA endonuclease SgrAI, the process of filamentation both accelerates its DNA cleavage activity and expands its DNA sequence specificity, thus allowing for many additional DNA sequences to be rapidly cleaved. Both outcomes—the acceleration of DNA cleavage and the expansion of sequence specificity—are proposed to regulate critical processes in bacterial innate immunity. However, the mechanistic bases underlying these events remain unclear. Herein, we describe two new structures of the SgrAI enzyme that shed light on its catalytic function. First, we present the cryo-EM structure of filamentous SgrAI bound to intact primary site DNA and Ca2+ resolved to ∼2.5 Å within the catalytic center, which represents the trapped enzyme–DNA complex prior to the DNA cleavage reaction. This structure reveals important conformational changes that contribute to the catalytic mechanism and the binding of a second divalent cation in the enzyme active site, which is expected to contribute to increased DNA cleavage activity of SgrAI in the filamentous state. Second, we present an X-ray crystal structure of DNA-free (apo) SgrAI resolved to 2.0 Å resolution, which reveals a disordered loop involved in DNA recognition. Collectively, these multiple new observations clarify the mechanism of expansion of DNA sequence specificity of SgrAI, including the indirect readout of sequence-dependent DNA structure, changes in protein–DNA interactions, and the disorder-to-order transition of a crucial DNA recognition element. 相似文献
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The pathogen Vibrio cholerae causes severe diarrheal disease in humans. This environmental inhabitant has two distinct life cycles, in the environment and in the human small intestine, in which it differs in its multiplication behavior and virulence expression. Anaerobiosis, limitation of some nutrient elements, and excess burden from host metabolism reactants are the major stresses for V. cholerae living in intestine, in comparison to conditions in the environment and laboratory medium. For an insight into the response of V. cholerae to different microenvironments, we cultured the bacteria in aerobic and anaerobic conditions, and compared the whole cell proteome by two-dimensional electrophoresis. Among the protein spots identified, some protein species involved in aerobic respiration and the nutrient carbohydrate transporters were found to be more abundant in aerobic conditions, and some enzymes for anaerobic respiration and some stress response proteins were found more abundant in anaerobic culture. One spot corresponding to flagellin B subunit was decreased in anaerobic conditions, which suggests correlation with the meticulous regulation of bacterial motility during infection in the host intestine. This proteome analysis is the starting point for in-depth understanding of V. cholerae behavior in different environments. 相似文献
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Mohammad Askari Amin Reza Nikpoor Fazel Gorjipour Mohsen Mazidi Mohammad Hosein Sanati Hajar Aryan Alireza Irani Khalil Ghasemi Falavarjani Hossein Nazari Kazem Mousavizadeh 《Reports of Biochemistry & Molecular Biology》2015,4(1):43-49