Ferredoxin reductase enhances heterologously expressed cytochrome CYP105D1 in Escherichia coli and Streptomyces lividans

The ability of two different ferredoxin reductases from Streptomyces coelicolor, to enhance the amount of active recombinant Streptomyces griseus soyC (CYP105D1) was investigated in both Escherichia coli and Streptomyces lividans. In E. coli a two-plasmid system and a single operon construct were used for expression of the CYP105D1 and the ferredoxin reductase(s) under the control of T7 promoters. Expression levels of CYP105D1 were found to range between 85 and 280 nmol l⁻¹ cell culture after prolonged growth. In S. lividans the CYP105D1 and its ferredoxin were cloned downstream of the Pact1 promoter in the E. coli/Streptomyces shuttle vector pBW160. The recombinant E. coli and S. lividans cells converted 7-ethoxycoumarin into 7-hydroxycoumarin efficiently. Expression of a ferredoxin reductase as an operon with CYP105D1 and its ferredoxin enhances the o-dealkylation of 7-ethoxycoumarin. Ferredoxin NADPH reductase was found to enhance the level of the active form of CYP105D1 monooxygenase when no substrate was present.     

Electrophysiological and Neurochemical Assessment of Selenium Alone or Combined with Carbamazepine in an Animal Model of Epilepsy

Biological Trace Element Research - The present study aims to evaluate the efficacy of selenium (Se) alone or combined with carbamazepine (CBZ) against the adverse effects induced by the...     

Efficient user-channel pairing with power-domain sum-rate maximization in opportunistic hybrid OFDMA-NOMA IoT systems

Non-orthogonal multiple access (NOMA) along with cognitive radio (CR) have been recently configured as potential solutions to fulfill the extraordinary demands of the fifth generation (5G) and beyond (B5G) networks and support the Internet of Thing (IoT) applications. Multiple users can be served within the same orthogonal domains in NOMA via power-domain multiplexing, whilst CR allows secondary users (SUs) to access the licensed spectrum frequency. This work investigates the possibility of combining orthogonal frequency division multiple access (OFDMA), NOMA, and CR, referred to as hybrid OFDMA-NOMA CR network. With this hybrid technology, the licensed frequency is divided into several channels, such as a group SUs is served in each channel based on NOMA technology. In particular, a rate-maximization framework is developed, at which user pairing at each channel, power allocations for each user, and secondary users activities are jointly considered to maximize the sum-rate of the hybrid OFDMA-NOMA CR network, while maintaining a set of relevant NOMA and CR constraints. The developed sum-rate maximization framework is NP-hard problem, and cannot be solved through classical approaches. Accordingly, we propose a two-stage approach; in the first stage, we propose a novel user pairing algorithm. With this, an iterative algorithm based on the sequential convex approximation is proposed to evaluate the solution of the non-convex rate-maximization problem, in the second stage. Results show that our proposed algorithm outperforms the existing schemes, and CR network features play a major role in deciding the overall network’s performance.