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991.
通过基因组相减从悬铃木突变体中分离缺失DNA 总被引:1,自引:0,他引:1
悬铃木(Platanus occidentalis L.)突变体是其种子经γ射线辐射培育出的一种营养生长正常而开花发育过程受阻的不育植株。经过10多年的观察研究证明:突变体的花芽不能形成是因为突变体细胞染色体有部分缺失。应用基因组相减技术分离这些在突变体中缺失但在野生型中存在的DNA,经过4次循环富集缺失片段后克隆了1个2.0 kb 的DNA 片段,DNA 杂交证明此片段只存在于野生型基因组中而不存在于突变体中。将基因组相减方法应用在复杂植物基因组中获得成功的研究还未见报道 相似文献
992.
CO2浓度倍增对谷子和紫花苜蓿叶绿体超微结构的效应 总被引:14,自引:0,他引:14
电镜观察结果表明,不同种类植物生长在相同倍增的高CO2 浓度条件下,其叶绿体超微结构彼此呈现出明显的差异. 最醒目的特征是淀粉粒的积累比对照的增加很多;类囊体膜系发生异变. 总体上,(1)淀粉粒,C4 植物谷子(Setaria italica)叶绿体比C3 植物紫花苜蓿(Medicago sativa)积累的更多. (2)淀粉粒较小且较少时,紫花苜蓿叶绿体基粒类囊体膜增多,与基质类囊体膜相间排列有序;谷子叶绿体的基粒垛及基粒类囊体膜数均增多,但基粒变小,基质类囊体膜变长,且有些膜出现膨胀甚至破损. (3)淀粉粒较大且积累过多时,紫花苜蓿叶绿体中尚可隐约见到由4~8 个类囊体膜组成的短小基粒零星分布于淀粉粒间;谷子叶绿体中几乎找不到可辨认的基粒和基质类囊体膜 相似文献
993.
水稻巯基蛋白酶抑制剂的纯化及其性质研究 总被引:2,自引:0,他引:2
水稻的糠皮和胚经生理盐水浸取、离心后的上清液加热至80℃处理10min,离心获得的上清液调pH至8.0,得到沉淀。沉淀溶解于0.01mol/LHCl,经透析冷冻干燥得水稻巯基蛋白酶抑制剂(CPI)粗品;粗品再经DEAE-Sepharose柱线性离子梯度洗脱和SephadexG-100柱分子筛层析,即可获得在PAGE、SDS-PAGE和HPLC上均为单一蛋白带的CPI样品。经上述步骤,CPI可被纯化58倍。经SephadexG-100和SDS-PAGE测定其分子量均为12000,N末端氨基酸为Pro,等电点5.6.水稻CPI经100℃处理10min后,其抑制活性无任何变化,在pH2.0~9.0之间,活性也不发生改变,但pH在9.0以上,其活性逐渐下降,水稻CPI对木瓜蛋白酶是一种高亲和性的抑制剂,它对木瓜蛋白酶和无花果蛋白酶有强抑制作用,对菠萝蛋白酶仅有弱抑制作用,但对胰蛋白酶则全无抑制作用;其抑制类型属竞争性抑制剂类型,K_i值约3.5×10 ̄(-8)mol/L对木瓜蛋白酶的抑制摩尔比约为1:1。 相似文献
994.
Isolation and characterization of chromosome-specific DNA sequences from a chromosome arm genomic library of common wheat 总被引:14,自引:0,他引:14
Bao Liu Gregorio Segal Juan Manuel Vega Moshe Feldman Shahal Abbo 《The Plant journal : for cell and molecular biology》1997,11(5):959-965
Isolation, physical mapping and polymorphism of chromosome-specific DNA sequences in wheat are reported. Following the microdissection of the long arm of chromosome 5B (5BL) of common wheat, its DNA was amplified by degenerate oligonucleotide-primed PCR and directly cloned into plasmid vectors. Characterization of the chromosome arm library showed that ∼55% of the inserts are of low-copy nature. Southern analysis using aneuploid lines of common wheat revealed that five of 11 low-copy inserts analyzed map to chromosome arm 5BL; four of these are 5BL-specific. By deletion mapping, the 5BL-specific sequences were located to sub- chromosome arm regions. Based on the hybridization patterns of three 5BL-specific sequences to DNA from a diverse collection of goat-grass ( Aegilops ) and wheat ( Triticum ) species, it was concluded that these sequences emerged at different times in the course of evolution of this group of plant species. 相似文献
995.
Yuhua Hao Wanguo Bao Meishan Jin Yuxiang Li Feng Wang 《The Korean journal of parasitology》2016,54(3):323-327
A man with only yellowing of the skin and eye sclera was diagnosed with clonorchiasis, which rarely manifested jaundice as the initial symptom. However, because of a lack of evidence for a diagnostic gold standard, the time until definitive diagnosis was more than a week. The diagnostic process relied on inquiring about the patient’s history, including the place of residence, dietary habits, and symptoms, as well as on serological findings, an imaging examination, and pathological findings. MRCP and CT results showed mild dilatation of intrahepatic ducts and increased periductal echogenicity. The eggs were ultimately found in stool by water sedimentation method after the negative report through direct smear. DNA sequencing of PCR production of the eggs demonstrated 98-100% homology with ITS2 of Clonorchis sinensis. After anti-parasite medical treatment, the patient’s symptoms were gradually relieved. Throughout the diagnostic procedure, besides routine examinations, the sedimentation method or concentration method could be used as a sensitive way for both light and heavy C. sinensis infection in the definite diagnosis. 相似文献
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Xuan Cai Lihui Zhu Xiaolian Chen Yongshuai Sheng Qi Guo Jian Bao Jianxiong Xu 《Cytotechnology》2016,68(4):713-724
We previously demonstrated that intestinal epithelial cell apoptosis in weaned piglets is much more serious than that observed in sucking piglets and is related to oxidative stress during weaning. It is difficult to study the apoptosis mechanisms only using in vivo methods because of the limit of existing research technology. An in vitro cellular system is required for piglet intestinal epithelial cell apoptosis research. In this study, a non-tumorigenic epithelial cell line, IPEC-J2 cells, was employed as a cell model. Hydrogen peroxide and xanthine/xanthine oxidase (X/XO) were both used and compared for apoptosis modeling. The concentrations of hydrogen peroxide and XO were selected and verified using cell viability analysis, the comet assay and flow cytometry. Intracellular ROS were measured using fluorescent probes. Additionally, the expression levels of the apoptosis-related genes Fas, Bcl-2, P53, Caspase 3, Caspase 8, and Caspase 9 were analyzed using quantitative RT-PCR. The results indicated the optimal modeling method is a final concentration of 0.5 mM H2O2 incubated with IPEC-J2 cells for 1 h at 37 °C in 5 % CO2 for hydrogen peroxide-induced apoptosis modeling, and a final concentration of 250 μM X/50 U/L XO incubated with IPEC-J2 cells for 6 h at 37 °C in 5 % CO2 for X/XO-induced apoptosis modeling. For the apoptotic pathway, the X/XO modeling method is more similar to 21 days weaning piglets. Therefore, we suggest that X/XO modeling with IPEC-J2 cells be used as an in vitro cell culture model for weaning piglet intestinal epithelial cell apoptosis. 相似文献
1000.
Qinhong Liu Cuijie Zhang Yihui Cheng Bo Zheng Manzhu Bao Xueping Shi 《Trees - Structure and Function》2016,30(4):1033-1043