全文获取类型
收费全文 | 33060篇 |
免费 | 2713篇 |
国内免费 | 2417篇 |
专业分类
38190篇 |
出版年
2024年 | 73篇 |
2023年 | 476篇 |
2022年 | 1066篇 |
2021年 | 1829篇 |
2020年 | 1122篇 |
2019年 | 1461篇 |
2018年 | 1397篇 |
2017年 | 1032篇 |
2016年 | 1383篇 |
2015年 | 2028篇 |
2014年 | 2386篇 |
2013年 | 2690篇 |
2012年 | 2979篇 |
2011年 | 2706篇 |
2010年 | 1634篇 |
2009年 | 1424篇 |
2008年 | 1658篇 |
2007年 | 1466篇 |
2006年 | 1290篇 |
2005年 | 1043篇 |
2004年 | 930篇 |
2003年 | 806篇 |
2002年 | 724篇 |
2001年 | 616篇 |
2000年 | 530篇 |
1999年 | 531篇 |
1998年 | 311篇 |
1997年 | 311篇 |
1996年 | 302篇 |
1995年 | 296篇 |
1994年 | 263篇 |
1993年 | 186篇 |
1992年 | 283篇 |
1991年 | 191篇 |
1990年 | 155篇 |
1989年 | 160篇 |
1988年 | 101篇 |
1987年 | 89篇 |
1986年 | 64篇 |
1985年 | 72篇 |
1984年 | 30篇 |
1983年 | 33篇 |
1982年 | 19篇 |
1981年 | 16篇 |
1980年 | 12篇 |
1979年 | 9篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
241.
242.
Distinct controls over the temporal dynamics of soil carbon fractions after land use change 总被引:2,自引:0,他引:2
Soil organic carbon (SOC), the largest terrestrial carbon pool, plays a significant role in soil‐related ecosystem services such as climate regulation, soil fertility and agricultural production. However, its fate under land use change is difficult to predict. A major issue is that SOC comprised of numerous organic compounds with potentially distinct and poorly understood turnover properties. Here we use spatiotemporal measurements of the particulate (POC), mineral‐associated (MOC) and charred SOC (COC) fractions from 176 trials involving changes in land use to assess their underlying controls. We find that the initial pool sizes of each of the three fractions consistently and dominantly control their temporal dynamics after changes in land use (i.e. the baseline effects). The effects of climate, soil physicochemical properties and plant residues, however, are fraction‐ and time‐dependent. Climate and soil properties show similar importance for controlling the dynamics of MOC and COC, while plant residue inputs (in term of their quantity and quality) are much less important. For POC, plant residues and management practices (e.g. the frequency of pasture in crop‐pasture rotation systems) are substantially more important, overriding the influence of climate. These results demonstrate the pivotal role of measuring SOC composition and considering fraction‐specific stabilization and destabilization processes for effective SOC management and reliable SOC predictions. 相似文献
243.
Jiayu Zhou Zhilin Liu Songfeng Wang Jie Li Yikui Li Wei-Kang Chen Ren Wang 《Environmental microbiology》2020,22(4):1421-1434
Lycoris radiata is a main source of Amaryllidaceae alkaloids; however, the low content of these alkaloids in planta remains a limit to their pharmaceutical development and utilization. The accumulation of secondary metabolites can be enhanced in plants inoculated with fungal endophytes. In this study, we analysed the diversity of culturable fungal endophytes in different organs of L. radiata. Then, by analysing the correlation between the detectable rate of each fungal species and the content of each tested alkaloid, we proposed several fungal candidates implicated in the increase of alkaloid accumulation. This was verified by inoculating these candidates to L. radiata plants. Based on the results of two independent experiments conducted in May 2018 and October 2019, the individual inoculation of nine fungal endophytes significantly increased the total content of the tested alkaloids in the entire L. radiata plants. This is the first study in L. radiata to show that fungal endophytes are able to improve the accumulation of various alkaloids. Therefore, our results provide insights into a better understanding of interactions between plants and fungal endophytes and suggest an effective strategy for enhancing the alkaloid content in the cultivation of L. radiata. 相似文献
244.
Yingfan Cai Xiaoyan Cai Qinglian Wang Ping Wang Yu Zhang Chaowei Cai Yanchao Xu Kunbo Wang Zhongli Zhou Chenxiao Wang Shuaipeng Geng Bo Li Qi Dong Yuqing Hou Heng Wang Peng Ai Zhen Liu Feifei Yi Minshan Sun Guoyong An Jieru Cheng Yuanyuan Zhang Qian Shi Yuanhui Xie Xinying Shi Ying Chang Feifei Huang Yun Chen Shimiao Hong Lingyu Mi Quan Sun Lin Zhang Baoliang Zhou Renhai Peng Xiao Zhang Fang Liu 《Plant biotechnology journal》2020,18(3):814-828
245.
246.
247.
248.
Xue‐Chen Wu Zhe Han Xin Hao Yi‐Tong Zhao Cheng‐Jie Zhou Xin Wen Cheng‐Guang Liang 《Molecular reproduction and development》2020,87(2):262-273
Phosphodiesterase (PDE)‐mediated reduction of cyclic adenosine monophosphate (cAMP) activity can initiate germinal vesicle (GV) breakdown in mammalian oocytes. It is crucial to maintain oocytes at the GV stage for a long period to analyze meiotic resumption in vitro. Meiotic resumption can be reversibly inhibited in isolated oocytes by cAMP modulator forskolin, cAMP analog dibutyryl cAMP (dbcAMP), or PDE inhibitors, milrinone (Mil), Cilostazol (CLZ), and 3‐isobutyl‐1‐methylxanthine (IBMX). However, these chemicals negatively affect oocyte development and maturation when used independently. Here, we used ICR mice to develop a model that could maintain GV‐stage arrest with minimal toxic effects on subsequent oocyte and embryonic development. We identified optimal concentrations of forskolin, dbcAMP, Mil, CLZ, IBMX, and their combinations for inhibiting oocyte meiotic resumption. Adverse effects were assessed according to subsequent development potential, including meiotic resumption after washout, first polar body extrusion, early apoptosis, double‐strand DNA breaks, mitochondrial distribution, adenosine triphosphate levels, and embryonic development. Incubation with a combination of 50.0 μM dbcAMP and 10.0 μM IBMX efficiently inhibited meiotic resumption in GV‐stage oocytes, with low toxicity on subsequent oocyte maturation and embryonic development. This work proposes a novel method with reduced toxicity to effectively arrest and maintain mouse oocytes at the GV stage. 相似文献
249.
Ju Zhou Qing Lan Wu Li Lin Yang Jing You Yan‐Mei Zhang Wei Ni 《Cell biology international》2020,44(1):108-116
To investigate the roles of tripartite motif containing 52 (TRIM52) in human hepatic fibrosis in vitro, human hepatic stellate cell line LX‐2 cells were transfected with hepatitis B virus (HBV) replicon to establish HBV‐induced fibrosis in LX‐2 cells, and then treated with small interfering RNA‐mediated knockdown of TRIM52 (siTRIM52). LX‐2 cells without HBV replicon transfection were treated with lentiviruses‐mediated overexpression of TRIM52 and phosphatase magnesium dependent 1A (PPM1A). Fibrosis response of LX‐2 cells were assessed by the production of hydroxyproline (Hyp) and collagen I/III, as well as protein levels of α‐smooth muscle actin (α‐SMA). PPM1A and phosphorylated (p)‐Smad2/3 were measured to assess the mechanism. The correlation between TRIM52 and PPM1A was determined using co‐immunoprecipitation, and whether and how TRIM52 regulated the degradation of PPM1A were determined by ubiquitination assay. Our data confirmed HBV‐induced fibrogenesis of LX‐2 cells, as evidenced by significant increase in Hyp and collagen I/III and α‐SMA, which was associated with reduction of PPM1A and elevation of transforming growth factor‐β (TGF‐β), p‐Smad2/3, and p‐Smad3L. However, those changes induced by HBV were significantly attenuated with additional siTRIM52 treatment. Similar to HBV, overexpression of TRIM52 exerted promoted effect in the fibrosis of LX‐2 cells. Interestingly, TRIM52 induced the fibrogenesis of LX‐2 cells and the activation of TGF‐β/Smad pathway were significantly reversed by PPM1A overexpression. Furthermore, our data confirmed TRIM52 as a deubiquitinase that influenced the accumulation of PPM1A protein, and subsequently regulated the fibrogenesis of LX‐2 cells. TRIM52 was a fibrosis promoter in hepatic fibrosis in vitro, likely through PPM1A‐mediated TGF‐β/Smad pathway. 相似文献
250.