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41.
Zhang Zaibao Ke Danxia Hu Menghui Zhang Chi Deng Lijun Li Yuting Li Jiuli Zhao Hai Cheng Lin Wang Lei Yuan Hongyu 《Plant molecular biology》2019,99(3):265-281
Plant Molecular Biology - PsLecRLK overexpression in rice provides tolerance against salinity stress and cause upregulation of SOS1 pathway genes, which are responsible for extrusion of excess Na+... 相似文献
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An animal (BALB/c mice) model of catalpol associated with bronchial asthma in vivo was established, and the effects of catalpol and its relationship with cytokines were investigated. A total of 30 adult BALB/c mice were randomly divided into a positive control group, a model group, and a catalpol group, with 10 mice in each group. The lung function of mice, the cell count, and the cytokine concentrations in bronchoalveolar lavage fluid (BALF) were detected. The levels of cytokines [interleukin 4 (IL-4), interleukin 5 (IL5), and interferon gamma (IFN-γ)] in BALF were measured with enzyme-linked immunosorbent assay methods. The total number of cells in the BALF of the group treated with catalpol was significantly lower than the model group. After treatment with catalpol, the eosinophils and neutrophils of the mice were remarkably reduced compared with the model group. The malondialdehyde content in the lung tissue homogenate of the mice was also decreased in the catalpol group. The cytokines IL-5 and IL-4 exhibited a similar tendency: the concentrations of IL-4 and IL-5 for the catalpol group were dramatically decreased compared with the model group. However, the IFN-γ concentration for the catalpol group was higher than the model group. The results indicated that IL-5 may involve in the pathologic process of asthma-like IL-4, and an inflammatory reaction may still exist in the airway during the remission stage of asthma. The imbalances of the cytokine network might be an important molecular basis in the asthma pathogenesis. It is suggested that catalpol may be a potential drug for the clinical treatment of asthma. 相似文献
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Mengmeng Jiang Hai Xie Chaoyang Zhang Tianqin Wang Haibin Tian Lixia Lu JingYing Xu GuoTong Xu Lin Liu Jingfa Zhang 《Journal of cellular and molecular medicine》2022,26(4):1229
The concept of diabetic retinopathy (DR) has been extended from microvascular disease to neurovascular disease in which microglia activation plays a remarkable role. Fractalkine (FKN)/CX3CR1 is reported to regulate microglia activation in central nervous system diseases. To characterize the effect of FKN on microglia activation in DR, we employed streptozotocin‐induced diabetic rats, glyoxal‐treated R28 cells and hypoxia‐treated BV2 cells to mimic diabetic conditions and explored retinal neuronal apoptosis, reactive oxygen species (ROS), as well as the expressions of FKN, Iba‐1, TSPO, NF‐κB, Nrf2 and inflammation‐related cytokines. The results showed that FKN expression declined with diabetes progression and in glyoxal‐treated R28 cells. Compared with normal control, retinal microglia activation and inflammatory factors surged in both diabetic rat retinas and hypoxia‐treated microglia, which was largely dampened by FKN. The NF‐κB and Nrf2 expressions and intracellular ROS were up‐regulated in hypoxia‐treated microglia compared with that in normoxia control, and FKN significantly inhibited NF‐κB activation, activated Nrf2 pathway and decreased intracellular ROS. In conclusion, the results demonstrated that FKN deactivated microglia via inhibiting NF‐κB pathway and activating Nrf2 pathway, thus to reduce the production of inflammation‐related cytokines and ROS, and protect the retina from diabetes insult. 相似文献
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Yunqing Gu Jun Cao Xinyu Zhang Hai Gao Yuyan Wang Jia Wang Juan He Xiaoyi Jiang Jinlan Zhang Guanghui Shen Jie Yang Xichen Zheng Gaowei Hu Yuanfei Zhu Shujuan Du Yunkai Zhu Rong Zhang Jianqing Xu Fei Lan Di Qu Guoliang Xu Yun Zhao Dong Gao Youhua Xie Min Luo Zhigang Lu 《Cell research》2022,32(1):24-37
Host cellular receptors play key roles in the determination of virus tropism and pathogenesis.However,little is known about SARS-CoV-2 host receptors with the e... 相似文献
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柑桔原生质体辐射诱变筛选抗寒再生植株 总被引:3,自引:0,他引:3
用1161C/kg软X射线辐射“Page”桔柚(CitrusreticulataBlanco×C.grandisOsb.cv.Page)胚性愈伤组织分离的原生质体,经-11℃低温选择后,将存活的原生质体培养再生植株。研究表明,“Page”桔柚胚性愈伤组织原生质体在4128C/kg辐射剂量处理后几乎不能恢复分裂;2064C/kg辐射时分裂频率低,即使分裂也难以发育为胚状体;而1161C/kg辐射后,经-11℃低温处理仍有102%~169%的分裂频率,且部分细胞团能发育为胚状体。辐射的原生质体培养成胚状体后,经低温筛选,在改良的生芽培养基和生根培养基中萌发成为植株。再生植株中检测出2株的叶片原生质体的低温半致死温度(LT50)分别比对照低1.96℃和168℃。 相似文献
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MUC1/Y特异表位肽在大肠杆菌中的可溶性表达及其抗体的制备 总被引:1,自引:0,他引:1
为获得MUC1/Y的特异表位肽,制备抗体,用PCR扩增其编码序列,克隆到pGEX-2T中,转化DH5α感受态,0.2mmol/L IPTG诱导表达,超声破碎或BPER-TMⅡ试剂处理诱导菌,亲和层析和阴离子交换纯化目的蛋白,SDSPAGE及Western blotting鉴定;免疫家兔制备多抗,初步用于免疫组化分析。结果表明,转化菌经诱导后表达融合蛋白GSTY30,约占菌体总蛋白的20%,大多以可溶形式存在,与诱导温度无关。免疫家兔获得多抗,效价为1∶320 000,纯化的抗体具有特异性,可识别肿瘤细胞表面的MUC1/Y蛋白。所得蛋白和抗体可用于MUC1/Y的表达特征及其生物学功能研究。 相似文献
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红曲菌代谢产物中低极性组分的分离及表征 总被引:1,自引:0,他引:1
以石油醚∶醋酸乙酯 =4∶1(V/V)作为洗脱剂 ,采用柱层层析粗分离醇溶性红曲菌代谢产物中的低极性组分。经浓缩、结晶除去白色结晶后的浓缩液 ,用正己烷∶醋酸乙酯 =9∶1(V/V)作为展开剂进行薄层层析分离 ,在紫外灯下观察 ,从低极性组分中分离出六个组分 ,分别为 :具荧光组分、两个相隔较近的黄色组分、淡黄色具荧光组分、具浅蓝绿色荧光组分、具荧光组分。各组分的Rf 值分别为 :0 2 9、0 15、0 12、0 0 9、0 0 6、0 0 4。MS测定结果表明 ,Rf 值最大的具荧光组分可能为含有 OH及Br的共轭烯烃或脂肪酮 ,而在紫外灯下呈淡黄色组分为含有 OH的环状化合物。 相似文献