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991.
992.
影响类弹性蛋白多肽(ELPs)自组装成微球的因素较多,目前尚缺乏系统研究。以类弹性蛋白多肽[KV8F]n为对象,利用动态光散射仪测定了不同条件下其自组装成微球的粒径。结果表明:随着分子量的增加ELPs形成的微球粒径也随之增大,粒径的均一度减小;当盐浓度低于0.4 mol/L时,盐浓度的增加,微球粒径相应增加,而盐浓度高于0.4 mol/L则呈减少的趋势,但粒径均大于1.1μm;而当ELPs末端融合木聚糖酶和1,3-丙二醇氧化还原酶后,其自组装形成的微球粒径急剧减小,约为游离ELPs的1/10,分别为151.0 nm和174.2 nm。导致这种现象的原因可能是酶分子和ELPs通过静电引力相互作用后,酶分子的空间位阻妨碍了ELPs分子的聚集。 相似文献
993.
Li-bo Cheng Lei Cheng Hui-e Bi Zhi-qing Zhang Jin Yao Xiao-zhong Zhou Qin Jiang 《Biochemical and biophysical research communications》2014
Patients with age related macular degeneration (AMD) will develop vision loss in the center of the visual field. Reactive oxygen species (ROS)-mediated retinal pigment epithelium (RPE) cell apoptosis is an important contributor of AMD. In this study, we explored the pro-survival effect of α-melanocyte stimulating hormone (α-MSH) on oxidative stressed RPE cells. We found that α-MSH receptor melanocortin 1 receptor (MC1R) was functionally expressed in primary and transformed RPE cells. RPE cells were response to α-MSH stimulation. α-MSH activated Akt/mammalian target of rapamycin (mTOR) and Erk1/2 signalings in RPE cells, which were inhibited by MC1R siRNA knockdown. α-MSH protected RPE cells from hydrogen peroxide (H2O2)-induced apoptosis, an effect that was almost abolished when MC1R was depleted by siRNA. α-MSH-mediated S6K1 activation and pro-survival effect against H2O2 was inhibited by Akt inhibitors (perifosine, MK-2206 and LY294002). Further, mTOR inhibition by rapamycin, or by mTOR siRNA knockdown, diminished α-MSH’s pro-survival effect in RPE cells. Thus, Akt and its downstream mTOR signaling mediates α-MSH-induced survival in RPE cells. In summary, we have identified a new α-MSH–MC1R physiologic pathway that reduces H2O2-induced RPE cell damage, and might minimize the risk of developing AMD. 相似文献
994.
Biao Gong Cunjia Zhang Xiu Li Dan WenShuoshuo Wang Qinghua Shi Xiufeng Wang 《Biochemical and biophysical research communications》2014
Soil salinity and alkalinity are common constraints to crop productivity in low rainfall regions of the world. However, the physiological difference of plant response to these two stresses was short of deep investigation. This study has identified a set of differentially expressed proteins of tomato root exploring to NaCl and NaHCO3 stress by iTRAQ (isobaric tags for relative and absolute quantitation) assay. A total of 313 proteins responsive to NaCl and NaHCO3 were observed. Among these proteins, 70 and 114 proteins were up-regulated by salt and alkali stress, respectively. While down-regulated proteins were 80 in salt treatment and 83 in alkali treatment. Only 39 up-regulated proteins and 30 down-regulated proteins were shared by salt and alkali stresses. The majority of the down-regulated proteins accounted for metabolism and energy conversion, and the up-regulated proteins were involved in signaling or transport. Compared with salt stress, alkali stress down-regulated proteins related with the respiratory metabolism, fatty acid oxidative metabolism and nitrogenous metabolism of tomato roots, and up-regulated protein with the reactive oxygen species (ROS) scavenging and ion transport. This study provides a novel insight into tomato roots response to salt and alkali stress at a large translation level. 相似文献
995.
观察为期4周模拟失重对大鼠心肌收缩性能与收缩蛋白性质的影响,发现模拟失重大鼠左心室乳头肌等长收缩的力学特征发生下列变化:发展张力峰值降低29%(P〈0.01);达到张力峰值的时间延长10%(P〈0.05);舒张一半的时间缩短11%,但未达到显著水平(P〉0.05)。心肌力学参数的这些变化表明模拟失重使大鼠心肌收缩性能降低。进一步研究显示,模拟失重大鼠左室心肌肌原纤维Ca^2+,Mg^2+-ATP酶 相似文献
996.
997.
几种昆虫病毒交叉感染玉米螟的研究 总被引:1,自引:0,他引:1
银纹夜蛾NPV(P_aNPV)、柞蚕NPV(ApNPV)和赤松毛虫CPV(D_aCPV)可感染玉米螟。D_aCPV对玉米螟1龄幼虫的ID_(50)为5.9×10~5PIB/ml饲料,对幼虫的生长发育、蛹重、羽化率和成虫寿命有显著影响。D_aCPV主要侵染幼虫中肠柱状细胞,在细胞质中增殖;P_aNPV和A_pNPV主要侵染幼虫的体壁细胞和气管壁细胞。D_aCPV在玉米螟幼虫体内增殖后,多角体形态由正六角形变为锥形或四方形;成虫羽化时排出的蛹便可观察到多角体,病毒可传递给子代。利用其他昆虫病毒有防治玉米螟的可能性。 相似文献
998.
Daniel Paredes Jay A. Rosenheim Rebecca Chaplin‐Kramer Silvia Winter Daniel S. Karp 《Ecology letters》2021,24(1):73-83
Diversifying agricultural landscapes may mitigate biodiversity declines and improve pest management. Yet landscapes are rarely managed to suppress pests, in part because researchers seldom measure key variables related to pest outbreaks and insecticides that drive management decisions. We used a 13‐year government database to analyse landscape effects on European grapevine moth (Lobesia botrana) outbreaks and insecticides across c. 400 Spanish vineyards. At harvest, we found pest outbreaks increased four‐fold in simplified, vineyard‐dominated landscapes compared to complex landscapes in which vineyards are surrounded by semi‐natural habitats. Similarly, insecticide applications doubled in vineyard‐dominated landscapes but declined in vineyards surrounded by shrubland. Importantly, pest population stochasticity would have masked these large effects if numbers of study sites and years were reduced to typical levels in landscape pest‐control studies. Our results suggest increasing landscape complexity may mitigate pest populations and insecticide applications. Habitat conservation represents an economically and environmentally sound approach for achieving sustainable grape production. 相似文献
999.
Evidence for a single median fin-fold and tail in the Lower Cambrian vertebrate, Haikouichthys ercaicunensis 总被引:2,自引:0,他引:2
In this study, we illustrate an exceptionally well-preserved Haikouichthys ercaicunensis from the Lower Cambrian Chengjiang fauna that displays complete single dorsal, ventral and caudal fins. This 530-million-year old vertebrate is fish-shaped and characterized by a single median fin-fold, which is an essential trait of the initial vertebrate chordates. The radially orientated ray-like structures in its dorsal fin somewhat resemble but are probably not real radials seen in basal vertebrates, such as hagfishes and lampreys. The unique design of primitive fins and fin structures provides additional insights into the early evolution of vertebrates. 相似文献
1000.
AIMS: To investigate the distribution of the virulence of two Vibrio species among different strains obtained from the mariculture systems on the coast of Guangdong in China and the correlation between the virulence strains and the virulence genes among Vibrio alginolyticus. METHODS: Besides three strains, 72 V. alginolyticus strains and seven Vibrio parahaemolyticus strains were examined by PCR or semi-nested PCR for the virulence genes (tlh, trh, tdh, toxR, toxRS, ctxA, VPI). Additionally, the virulence of 18 V. alginolyticus strains was tested. SIGNIFICANCE AND IMPACT OF THE STUDY: Virulence genes homologous to those in the V. parahaemolyticus and Vibrio cholerae are widely distributed among V. alginolyticus and V. parahaemolyticus in the coastal mariculture systems in Guangdong, China. Some of the V. alginolyticus strains are pathogenic to aquatic animals, and might have derived their virulence genes from V. parahaemolyticus or V. cholerae, representing a possible reservoir of these genes. However, there is no correlation between presence and absence of the virulence genes used to investigate V. alginolyticus and its virulent strains. In this report, we also show that tlh is distributed among V. alginolyticus. 相似文献