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Summary When arsenate-resistant mutants are selected approximately 50 per cent of them are also consitutive for the synthesis of alkaline phosphatase and the Pi-binding protein. Some of these mutants are linked to ilv (phoS
- or phoT
-), others are linked to proC (phoR
-). One of the mutant strains linked to ilv lost the Pi-binding protein (the phoS gene product). Resistance to arsenate, constitutivty for alkaline phosphatase synthesis and loss of the Pi-binding protein occurred pleiotropically by the same phoS
- mutation. 相似文献
204.
Sigmund Schwimmer 《Economic botany》1954,8(2):99-113
The papaya tree and barley are the only flowering plants now being utilized to any extent for commercial production of enzymes. The papaya fruit yields papain, a protein-digesting enzyme which can be used to eliminate or modify protein in several industries. Barley is germinated to malt, which contains amylase, necessary for the conversion of starchy materials to fermentable sugars. Malt is used to a great extent in the brewing and distilling industries. Other plants, for example, alfalfa, oranges and beans, are used to a minor extent in the preparation of enzymes for specific industrial purposes. 相似文献
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Tuo Shi Gaofeng Niu Hagit Kvitt Xinqing Zheng Qiaoyun Qin Danye Sun Zhiliang Ji Dan Tchernov 《Molecular ecology resources》2021,21(1):137-152
Collectively called zooxanthellae, photosynthetic dinoflagellates in the family Symbiodiniaceae are typical endosymbionts that unequivocally mediate coral responses to environmental changes. Symbiodiniaceae are genetically diverse, encompassing at least nine phylogenetically distinct genera (clades A–I). The ribosomal internal transcribed spacer 2 (ITS2) region is commonly utilized for determining Symbiodiniaceae diversity within clades. However, ITS2 is often inadvertently interpreted together with the tailing part of the ribosomal RNA genes (5.8S and 28S or equivalent), leading to unresolved taxonomy and equivocal annotations. To overcome this hurdle, we mined in GenBank and expert reference databases for ITS2 sequences of Symbiodiniaceae having explicit boundaries with adjacent rRNAs. We profiled a Hidden Markov Model of the ITS2‐proximal 5.8S‐28S rRNA interaction, which was shown to facilitate the delimitation of Symbiodiniaceae ITS2 from GenBank, while considerably reducing sequence ambiguity and redundancy in reference databases. The delineation of ITS2 sequences unveiled intra‐clade sequence diversity and inter‐clade secondary structure conservation. We compiled the clean data into a non‐redundant database that archives the largest number of Symbiodiniaceae ITS2 sequences known to date with definite genotype/subclade representations and well‐defined secondary structures. This database provides a fundamental reference catalog for consistent and precise genotyping of Symbiodiniaceae and a tool for automated annotation of user‐supplied sequences. 相似文献
207.
A novel method for the assay of tomatine in plant extracts is described. It is based on precipitation of tomatine with cholesterols-4-14C and determination of the radioactivity remaining in solution by scintillation counting. Pigments that cause quenching are removed by chromatography on Sephadex LH20. 相似文献
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