全文获取类型
收费全文 | 269篇 |
免费 | 20篇 |
出版年
2023年 | 3篇 |
2022年 | 7篇 |
2021年 | 3篇 |
2020年 | 4篇 |
2019年 | 15篇 |
2018年 | 8篇 |
2017年 | 4篇 |
2016年 | 5篇 |
2015年 | 15篇 |
2014年 | 12篇 |
2013年 | 24篇 |
2012年 | 17篇 |
2011年 | 16篇 |
2010年 | 5篇 |
2009年 | 7篇 |
2008年 | 10篇 |
2007年 | 7篇 |
2006年 | 4篇 |
2003年 | 5篇 |
2002年 | 4篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1982年 | 7篇 |
1981年 | 2篇 |
1977年 | 2篇 |
1976年 | 4篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1973年 | 8篇 |
1972年 | 4篇 |
1971年 | 5篇 |
1970年 | 7篇 |
1969年 | 5篇 |
1968年 | 4篇 |
1966年 | 5篇 |
1965年 | 3篇 |
1964年 | 2篇 |
1960年 | 4篇 |
1958年 | 2篇 |
排序方式: 共有289条查询结果,搜索用时 31 毫秒
141.
MM Fierro L Cruz-López D Sánchez R Villanueva-Gutiérrez R Vandame 《Neotropical Entomology》2012,41(2):95-104
We recorded stingless bee colony abundance and nesting habits in three sites with different anthropogenic activities in the Soconusco region of Chiapas, Mexico: (1) agroforestry (7 hacacao crop), (2) grassland (12?ha), and (3) urban area (3?ha). A total of 67 nests were found, representing five stingless bee species, Tetragonisca angustula angustula (Lepeletier), Trigona fulviventris (Guérin), Scaptotrigona mexicana (Guérin), Scaptotrigona pectoralis (Dalla Torre), and Oxytrigona mediorufa (Cockerell). The most abundant stingless bee in each site was T. angustula angustula (>50%). The primary tree species used by the bees were Ficus spp. (Moraceae, 37.8%) and Cordia alliodora (Boraginaceae, 13.5%). The nest entrance height of T. angustula angustula (96?±?19?cm) was different than the other species, and this bee was the only one that used all different nesting sites. Volatiles analyzed by gas chromatography from pollen collected by the stingless bees differed between bee species, but were highly similar in respect to the fragrances of the pollen collected by the same species at any site. Our data indicate that T. angustula angustula experienced low heterospecific and high intraspecific foraging overlap especially in the urban site. We observed cluster spatial distribution in grassland and in agroforestry sites. In the urban site, T. angustula angustula presented random distribution tended to disperse. Trigona fulviventris was the only overdispersed and solitary species. 相似文献
142.
143.
Dr. P. T. Brey R. A. Lebrun B. Papierok H. Ohayon S. Vennavalli J. Hafez 《Cell and tissue research》1988,253(1):245-250
Summary The adherence of zoospores of Lagenidium giganteum to the cuticle of mosquito larvae is the initial step in the infection process. Subsequently, a germ tube penetrates the integument, inducing a rapid melanization of the injured cuticle and epidermis. After entering the hemocoel the developing hyphae are occasionally encapsulated locally. This process is slow (6 to 12 h postincubation) and most frequently cell-free, although it can be mediated by circulating hemocytes. Sporadic hemocyte mediation of the humoral encapsulation process in larval stages of Culicidae adds a previously unreported dimension to this unusual type of defense reaction. The defense reactions of larvae of Aedes aegypti were ineffective against observed infection by Lagenidium giganteum. 相似文献
144.
Barkawi AH Hafez YM Ibrahim SA Ashour G El-Asheeri AK Ghanem N 《Animal reproduction science》2009,116(3-4):326-334
Data of 56 normal and 9 abnormal estrous cycles were collected from 9 Egyptian buffaloes (Bublus bublis) to describe the follicular growth wave pattern. Heat was checked twice daily while, ovaries were scanned daily to monitor the patterns of follicular waves. Day of ovulation was determined when the largest follicle was replaced by corpus haemorrhgicum (CH). Number of waves/cycle, day of emergence of the follicular wave, characteristics of the dominant follicle and corpus luteum (CL) growth features were monitored. Buffaloes displayed mainly two types of follicular waves; two (46.4%) and three (53.6%). In cycles of three wave pattern, time of emergence of the 1st wave post-heat was longer (P < 0.05) and number of recruited follicles/wave were larger (P < 0.05) compared to the corresponding values of the two wave pattern. Number of recruited follicles in early follicular waves (1st or the 2nd) had larger number (P < 0.05) compared to the subsequent ones. Follicles that reached ovulation in both types of estrous cycle had shorter life-span (P < 0.05) than the previous ones. Life-span of CH, growing and regressed CL were 3.6 ± 0.6, 11.2 ± 0.8 and 4.4 ± 0.5 days, respectively with no difference in both types of follicular wave. Three types of ovarian disorders were observed. Follicular waves and CL growth features showed unique pattern for each individual. These results demonstrate that buffaloes display two main types of follicular waves with dominance of three wave type. 相似文献
145.
Yi Sui Hai-Lu Zhao Heung Man Lee Jing Guan Lan He Fernand MM Lai Peter CY Tong Juliana CN Chan 《Translational oncology》2009,2(4):258-263
Nephrectomized rats have widely been used to study chronic renal failure. Interestingly, renal cell carcinoma occurred in the remnant kidney after uninephrectomy (UNX). In this study, we probed insulin-like growth factor (IGF)-1 signaling pathway in UNX-induced renal cancer. Adult male Sprague-Dawley rats were randomized into two groups: UNX rats (n = 22) and sham-operated rats (n = 12). Rats were killed at 3, 7, and 10 months. After 7 months after nephrectomy, the UNX rats developed renal cell carcinoma with increased expression of proliferating cell nuclear antigen, and 68.2% (15/22) of the animals exhibited invasive carcinoma. Western blot demonstrated significant down-regulation of IGF binding protein 3 contrasting with the up-regulation of protein kinase Cζ and Akt/protein kinase B in the renal cancer tissues. These findings indicate a unique rat model of UNX-induced renal cancer associated with enhanced IGF-1 signaling pathway. 相似文献
146.
The Burgers equation is obtained to study the characteristics of nonlinear propagation of ionacoustic shock, singular kink, and periodic waves in weakly relativistic plasmas containing relativistic thermal ions, nonextensive distributed electrons, Boltzmann distributed positrons, and kinematic viscosity of ions using the well-known reductive perturbation technique. This equation is solved by employing the (G'/G)-expansion method taking unperturbed positron-to-electron concentration ratio, electron-to-positron temperature ratio, strength of electrons nonextensivity, ion kinematic viscosity, and weakly relativistic streaming factor. The influences of plasma parameters on nonlinear propagation of ion-acoustic shock, periodic, and singular kink waves are displayed graphically and the relevant physical explanations are described. It is found that these parameters extensively modify the shock structures excitation. The obtained results may be useful in understanding the features of small but finite amplitude localized relativistic ion-acoustic shock waves in an unmagnetized plasma system for some astrophysical compact objects and space plasmas. 相似文献
147.
Sefika C Mizrak Bart M Gadella Hatice Erdost Aytekin Ozer Ana MM van Pelt Federica MF van Dissel-Emiliani 《Reproductive biology and endocrinology : RB&E》2008,6(1):1-9
Background
The placenta is an important site for iron metabolism in humans. It transfers iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to distinguish placental dysfunction.Methods
In the experimental study we used antibody preparation, mass spectrometric analysis, biochemical and immunocytochemical methods for characterization of transferrin expression on the human choriocarcinoma cell line JAR (JAR cells), placental lysates, and cryostat sections. Newly designed monoclonal antibody TRO-tf-01 to human transferrin was applied on human placentae from normal (n = 3) and abnormal (n = 9) pregnancies.Results
Variations of transferrin expression were detected in villous syncytiotrophoblast, which is in direct contact with maternal blood. In placentae from normal pregnancies, the expression of transferrin in the syncytium was significantly lower (p < 0.001) when compared to placentae from abnormal ones (gestational diabetes, pregnancy induced hypertension, drug abuse).Conclusion
These observations suggest that in the case of abnormal pregnancies, the fetus may require higher levels of transferrin in order to prevent iron depletion due to the stress from the placental dysfunction. 相似文献148.
Linkage analysis identifies the thyroglobulin gene region as a major locus for familial congenital hypothyroidism 总被引:1,自引:0,他引:1
Ahlbom BE Yaqoob M Gustavsson P Abbas HG Annerén G Larsson A Wadelius C 《Human genetics》2002,110(2):145-147
Congenital hypothyroidism affects 1/3000-4000 newborns and it has been estimated that 10-20% are familial cases with an autosomal recessive mode of inheritance. Previous studies of mostly individual cases have led to the identification of mutations in a number of genes, indicating that it is a genetically heterogeneous disease, but no major gene has been identified. In the present investigation, a population-based sample of 23 families with autosomal recessive congenital hypothyroidism, but no signs of goitre, were subject to linkage analysis. When markers located close to the thyroglobulin gene on chromosome 8q24 were used in a two-point analysis allowing for heterogeneity, a Z(max) of 4.10 was obtained with the microsatellite marker D8S557, indicating heterogeneity with 43% of the families being linked. A multipoint analysis using the markers D8S557 and D8S1835 gave a Z(max) of 3.51, assuming homogeneity. There was significant evidence of heterogeneity with 44.5% of the families being linked. The results indicate that a gene in 8q24 is a common cause of familial congenital hypothyroidism. Since thyroglobulin is essential for thyroid physiology, the gene encoding this protein is the obvious candidate for mutation analysis in the linked families. 相似文献
149.
OBJECTIVE: To describe the clinical, biological and molecular data in a large Egyptian kindred with 5alpha-reductase deficiency. PATIENTS AND METHODS: Three patients with ambiguous genitalia were referred at the ages of 20, 9 and 2 years, respectively. In all cases, parents were first cousins. Basal and post-HCG stimulation plasma levels of testosterone and dihydrotestosterone were determined. Direct sequencing and restriction site analysis were applied for patient and family study. RESULTS: A homozygous alanine to glutamic acid substitution at position 62 (A62E) was found in the three patients. The parents and two XX sisters were heterozygous while a third XX sibling was normal. CONCLUSION: We report a new mutation of the 5alpha-reductase type 2 gene. The presence of this mutation in all studied patients and their parents suggests its causative role in 5alpha-reductase deficiency. Identification of the mutation enabled genetic counselling for three XX individuals. 相似文献
150.
Cholesteryl hemisuccinate (CHEMS) is an acidic cholesterol ester that self-assembles into bilayers in alkaline and neutral aqueous media and is commonly employed in mixtures with dioleoylphosphatidylethanolamine (DOPE) to form 'pH sensitive' fusogenic vesicles. We show here that CHEMS itself exhibits pH sensitive polymorphism. This is evident from the fusogenic properties of large unilamellar vesicles (LUV) composed of CHEMS and direct visualization employing freeze-fracture electron microscopy. Below pH 4.3, LUV composed of CHEMS undergo fusion as monitored by lipid mixing assays and freeze-fracture electron micrographs reveal the characteristic striated signature of H( parallel) phase lipid. It is suggested that the pH dependent phase preferences of CHEMS contribute to the pH sensitivity of LUV composed of mixtures of CHEMS and DOPE. 相似文献