全文获取类型
收费全文 | 410篇 |
免费 | 34篇 |
国内免费 | 9篇 |
专业分类
453篇 |
出版年
2021年 | 3篇 |
2020年 | 3篇 |
2017年 | 6篇 |
2015年 | 6篇 |
2014年 | 4篇 |
2013年 | 8篇 |
2012年 | 9篇 |
2011年 | 11篇 |
2010年 | 14篇 |
2009年 | 18篇 |
2008年 | 17篇 |
2007年 | 9篇 |
2006年 | 13篇 |
2005年 | 3篇 |
2004年 | 10篇 |
2003年 | 7篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 11篇 |
1998年 | 6篇 |
1997年 | 6篇 |
1996年 | 12篇 |
1995年 | 5篇 |
1994年 | 4篇 |
1992年 | 3篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1983年 | 5篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1978年 | 5篇 |
1974年 | 5篇 |
1972年 | 4篇 |
1971年 | 4篇 |
1959年 | 7篇 |
1958年 | 23篇 |
1957年 | 26篇 |
1956年 | 26篇 |
1955年 | 22篇 |
1954年 | 22篇 |
1953年 | 13篇 |
1952年 | 13篇 |
1951年 | 10篇 |
1950年 | 9篇 |
1949年 | 2篇 |
1948年 | 2篇 |
1912年 | 2篇 |
排序方式: 共有453条查询结果,搜索用时 0 毫秒
81.
82.
83.
84.
85.
本研究旨在探讨谷糠结合态多酚(bound phenol of inner shell,BPIS)发挥抗乳腺癌细胞活性的作用机制。首先采用细胞计数法检测BPIS对乳腺癌细胞以及正常乳腺细胞活性的影响;然后综合运用SEA、SIB以及GeneCards等数据库获得BPIS和乳腺癌的相关靶点,并分析活性成分与作用靶点的互作网络以及通路。本研究筛选得到BPIS抗乳腺癌相关靶点39个,主要涉及糖脂代谢和细胞自噬等生物过程以及MAPK、PI3K/AKT、FoxO等多条信号通,表明BPIS抗乳腺癌是多成分、多靶点、多通路协同作用的过程,而与细胞死亡相关的细胞自噬很可能在BPIS抑制乳腺癌过程中发挥主要作用。 相似文献
86.
M.E. Hadwiger S.R. Torchia S. Park M.E. Biggin C.E. Lunte 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1996,681(2):241
Isoproterenol is a chiral catecholamine with a half-life of elimination of less than 10 min. In order to study the pharmacokinetics of this compound using microdialysis sampling, an analytical method was needed which could resolve the individual enantiomers of isoproterenol and required less than 1 μl of sample. A capillary electrophoretic method using a run buffer containing methyl-O-β-cyclodextrin as a chiral recognition agent was developed which could resolve the enantiomers of isoproterenol. The detection limits using UV absorbance detection were found to be too high to determine the concentration of isoproterenol in plasma for a sufficient time following administration to establish the pharmacokinetics. The detection limits were decreased three orders of magnitude to 3 ng/ml by using an amperometric detector. The detection limits were decreased to 0.6 ng/ml using an on-column concentration technique in which peak stacking was accomplished by following the sample injection with a plug of acid. 相似文献
87.
88.
89.
New epidemiological features on animal trypanosomiasis by molecular analysis in the pastoral zone of Sideradougou, Burkina Faso 总被引:2,自引:0,他引:2
T. LEFRANÇOIS P. SOLANO S. DE LA ROCQUE† Z. BENGALY J. M. REIFENBERG I. KABORE & D. CUISANCE 《Molecular ecology》1998,7(7):897-904
A multidisciplinary work was undertaken in the agropastoral zone of Sidéradougou, Burkina Faso to try to elucidate the key factors determining the presence of tsetse flies. In this study the PCR was used to characterize trypanosomes infecting the vector ( Glossina tachinoides and Glossina palpalis gambiensis ) and the host, i.e. cattle. A 2-year survey involved dissecting 2211 tsetse of the two Glossina species. A total of 298 parasitologically infected tsetse were analysed by PCR. Trypanosoma vivax was the most frequently identified trypanosome followed by the savannah type of T. congolense and, to a lesser extent, the riverine forest type of T. congolense , and by T. brucei . No cases of T. simiae were found. From the 107 identified infections in cattle, the taxa were the same, but T. congolense savannah type was more frequent, whereas T. vivax and T. congolense riverine forest types were found less frequently. A correlation was found between midgut infection rates of tsetse, nonidentified infections and reptile bloodmeals. These rates were higher in G.p. gambiensis , and in the western part of the study area. T. vivax infections were related to cattle bloodmeals, and were more frequent in G. tachinoides and in the eastern study area. The PCR results combined with bloodmeal analysis helped us to establish the relationships between the vector and the host, to assess the trypanosome challenge in the two parts of the area, to elucidate the differences between the two types of T. congolense , and to suspect that most midgut infections were originating from reptilian trypanosomes. 相似文献
90.
Fruit morphology in Tragopogon L. (Compositae: Lactuceae) from the Iberian Peninsula 总被引:2,自引:0,他引:2
G. BLANCA C. DÍAZ DE LA GUARDIA 《Botanical journal of the Linnean Society. Linnean Society of London》1997,125(4):319-329
Achenes of all the Tragopogon species from the Iberian Peninsula were examined by means of scanning-electron microscopy and stereomicroscopy. The achenes of the eight species are described, illustrated and compared. The results are contrasted with the systematics of this genus. The isolated position of T. lamottei with regard to the other seven species is noted. A key is provided to enable the different species to be distinguished. 相似文献