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81.
Hachiro Inokuchi Kazunori Kondo Masami Yoshimura Haruo Ozeki 《Molecular & general genetics : MGG》1990,223(3):433-437
Summary A UGA suppressor derived from a glutamine tRNA gene of Escherichia coli K 12 was isolated and characterized. Phages carrying the suppressor su+2UGA could be obtained only from a hybrid transducing phage, h
80
cI
857psu
+2oc, but not from the original transducing phage cI
857psu
+2oc. By DNA sequence analysis, it was found that the su
+2 UGA suppressor obtained has two mutations; one is in the anticodon (TTATCA), as expected, and the other (CT) is at the 7th position from the 3 end of tRNA
2
Gln
. The significance of these mutations and the lethal effect on phage of the increased amounts of UGA suppressor tRNAs are discussed. 相似文献
82.
Yukako Nakayama Koichi Kawahara Mitsuru Yoneyama Takeru Hachiro 《Biological Rhythm Research》2005,36(4):315-324
Cultured cardiac myocytes from neonatal rats show spontaneous and rhythmic contractions. The intracellular concentration of free Ca2 + also changes rhythmically, associated with the rhythmic contraction of myocytes (Ca2 + oscillation). This study aims to elucidate whether spontaneous rhythmic contraction affects the dynamics of intracellular Ca2 + oscillation in cultured cardiac myocytes. In cultures at four days in vitro (4 DIV), spontaneous Ca2 + oscillation was synchronized among myocytes. Treatment of cultures with an uncoupler of E - C coupling resulted in a cessation of the spontaneous contraction of cardiac myocytes, but did not abolish the Ca2 + oscillation. The intercellular synchronization of intracellular Ca2 + oscillation persisted, and both the intervals and the fluctuation of the oscillation tended to increase after the termination of rhythmic contraction. The present study demonstrated that mechanical factors associated with rhythmic contraction did not affect the intercellular synchronization of intracellular Ca2 + oscillation, but possibly contributed to the stability of the oscillatory rhythm. 相似文献
83.
Keita Takahashi Tetsuhiro Niidome Akinori Akaike† Takeshi Kihara Hachiro Sugimoto 《Journal of neurochemistry》2009,109(5):1324-1337
The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) is known to activate the ER, which is termed ER stress. Here, we demonstrated that amyloid precursor protein (APP) is a novel mediator of ER stress-induced apoptosis through the C/EBP homologous protein (CHOP) pathway. Expression of APP mRNA was elevated by tunicamycin- or dithiothreitol-induced ER stress. The levels of C83 and APP intracellular domain (AICD) fragments, which are cleaved from APP, were significantly increased under ER stress, although the protein level of full-length APP was decreased. Cellular viability was reduced in APP-over-expressing cells, which was attenuated by treatment with a γ-secretase inhibitor, N -[ N -(3,5-difluorophenacetyl)-L-alanyl]- S -phenylglycine t -butyl ester (DAPT). Cellular viability was also reduced in AICD-FLAG-over-expressing cells. The mRNA and protein levels of CHOP, an ER stress-responsive gene, were remarkably increased by APP over-expression, which was attenuated by treatment with DAPT. CHOP mRNA induction was also found in AICD-FLAG-over-expressing cells. Cell death and CHOP up-regulation by ER stress were attenuated by APP knockdown. Data obtained with a luciferase assay and chromatin immunoprecipitation assay indicated that AICD associates with the promoter region of the CHOP gene. In conclusion, ER stress-induced APP undergoes α- and γ-secretase cleavage and subsequently induces CHOP-mediated cell death. 相似文献
84.
85.
86.
87.
Jun Takahashi Ichiro Hijikuro Takeshi Kihara Modachur G. Murugesh Shinichiro Fuse Ryo Kunimoto Yoshinori Tsumura Akinori Akaike Tetsuhiro Niidome Yasushi Okuno Takashi Takahashi Hachiro Sugimoto 《Bioorganic & medicinal chemistry letters》2010,20(5):1718-1720
We synthesized a series of N1-substituted norcymserine derivatives 7a–p and evaluated their anti-cholinesterase activities. In vitro evaluation showed that the pyridinylethyl derivatives 7m–o and the piperidinylethyl derivative 7p improved the anti-butyrylcholinesterase activity by approximately threefold compared to N1-phenethylnorcymserine (PEC, 2). A quantitative structure-activity relationship (QSAR) study indicated that logS might be a key feature of the improved compounds. 相似文献
88.
Terauchi T Doko T Yonaga M Kajiwara A Niidome T Taguchi R Kume T Akaike A Sugimoto H 《Bioorganic & medicinal chemistry letters》2006,16(19):5080-5083
Analogues of serofendic acid were prepared and their protective effects against L-glutamate (Glu)-induced neurotoxicity were examined using primary cultures of rat cortical neurons. Some analogues exhibited similar neuroprotective activity to that of serofendic acid. 相似文献
89.
Nakano T Shimanuki T Matsushita M Koyama I Inoue I Katayama S Alpers DH Komoda T 《Biochemical and biophysical research communications》2006,341(1):33-38
Serum levels of intestinal alkaline phosphatase (IAP), a protein implicated in transcellular transport of chylomicrons, vary among ABO blood groups. In rat enterocytes, IAP is associated with chylomicron secretion, but the rat expresses only blood group A. It is not known whether chylomicron secretion may be affected in humans who express multiple blood group types. Serum samples from 40 healthy subjects were obtained after overnight fast and 3h after a high-fat meal, and assayed for IAP and apolipoprotein B-48 (apoB-48), both proteins exclusive to intestine, although only apoB-48 is found in chylomicrons. The two proteins were greater in subjects without blood antigen A (B and O) than in those with this antigen (A and AB); 2.4- and 4.7-fold for IAP and 1.5- and 2.0-fold for apoB-48 before and after the meal, respectively. Moreover, IAP and apoB-48 levels were strongly correlated in the subjects with the secretor phenotype (r > 0.81). These results indicate that IAP is strongly involved in chylomicron formation and fatty acid metabolism might change among ABO blood type. In addition, ABO blood type classification in apoB-48 measurement would improve the diagnostic value in the evaluation of metabolic syndrome. 相似文献
90.
Yamada Y Yamauchi D Yokoo M Ohinata K Usui H Yoshikawa M 《Bioscience, biotechnology, and biochemistry》2008,72(1):257-259
In this study, we found that novokinin (Arg-Pro-Leu-Lys-Pro-Trp), a potent hypotensive peptide acting through the AT(2) receptor, has vasorelaxing activity in the mesenteric artery isolated from spontaneously hypertensive rats. The vasorelaxing activity was significantly blocked by PD123319, indomethacin, and CAY10441, which are an AT(2) receptor antagonist, a cyclooxygenase inhibitor, and an IP receptor antagonist, respectively. N(G)-nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthase, did not block the vasorelaxing activity. These results suggest that the vasorelaxing activity of novokinin, which contributes to the hypotensive effect, is mainly mediated by prostaglandin I(2) (prostacyclin) and the IP receptor downstream of the AT(2) receptor. 相似文献