Acute 17β-Estradiol Pretreatment Protects Against Abdominal Aortic Occlusion Induced Spinal Cord Ischemic-Reperfusion Injury

Postoperative neurologic deficit due to spinal cord ischemia-reperfusion (I/R) injury is the most devastating complication following thoracoabdominal aortic aneurysm repairs. The protective potential for 17β-Estradiol has not been yet studied in such injury. In this study, ischemia induction for 18 min in male New Zealand White rabbits resulted in the highest percentage (80%) of biphasic paraplegic outcome assessed by Tarlov’s score. Acute Estradiol pretreatment (1 mg/kg, i.p., 30 min before I/R induction) altered this outcome and significantly prevented the worsening pattern of neurologic deficits over 48 h of observation. Histopathologic and oxidative stress evaluations of lumbar spinal cords taken in delayed permanent paraplegic phase (48 h after ischemia induction), further confirmed protective efficacy of Estradiol in such context. In western blot analysis, the expression of cleaved caspase-3 and heat shock protein 70 declined in Estradiol pretreated group compared to ischemic control group. TUNEL assay also showed the efficacy of Estradiol to abate motor neuron apoptosis. Interestingly, Estradiol respectively increased and decreased the expression of Cyclooxygenase (COX)-1 and COX-2, to a significant extent. Estradiol, exerting its protection through affecting one or a combination of involved biochemical factors can constitute a potential candidate to protect against thoracoabdominal aortic aneurysm repairs induced spinal cord I/R injury.     

Evaluation of Physicochemical Activity of Anticancer Fusion Proteins; Enterocin A- R type pyocin-Lactocin-Ligand Against Gastric Cancer Cell Line by Real-Time RT PCR Technique

International Journal of Peptide Research and Therapeutics - Gastric cancer treatment remains a major challenge. There are many reports on the positive efficacy of Bacteriocins associated with...     

Cholesteryl ester transfer protein (CETP) ?629C/A polymorphism and it,s effects on the serum lipid levels in metabolic syndrome patients

Metabolic syndrome is a relatively common disorder with significant morbidity worldwide. Cholesteryl ester transfer protein (CETP) plays a central role in the metabolism of lipoproteins. In this study the effect of -629C/A polymorphism on the concentration of CETP and plasma lipids pattern was elicited in metabolic syndrome patients and control subjects. For this, a sample of 200 patients diagnosed with metabolic syndrome disorder was studied in comparison with 200 healthy controls. This study was performed by using polymerase chain reaction and restriction fragment length polymorphisms. Genotype distribution and allelic frequencies were determined and compared in metabolic syndrome and healthy controls. To determine the relationship between -629C/A polymorphism and lipid levels, lipids and CETP concentration were measured in metabolic syndrome and normal subjects. The results showed a significant difference between two groups in terms of FBS, cholesterol, TG, HDL-C, LDL-C levels as well as BMI, waist circumference, systolic and diastolic blood pressure. The genotype frequencies for this polymorphism differed significantly between metabolic syndrome patients and controls (in control group: CC%?20.5, CA%?76, AA%?3.5 and in patient group: CC%?28.5, CA%?53.5, AA%?18) (p?相似文献   

A comparison of back-calculated lengths of silver carp derived from bony structures

Linear relationships were found between body length–scale radius, body length-cleithrum length and body length-urohyal length ( r =0·949, 0·984 and 0·974 respectively) in silver carp Hypophthalmichthys molitrix. Out of 180 comparisons, which were made between different methods of back-calculating length and ageing structures at ages 2-7 years, the differences were not significant ( P ≥ 0·05) in 132 (73·3%) cases. There were no significant differences among different methods in older age classes and between the Fraser-Lee method and body proportional method (BPH) at all ages. The results demonstrated that in all structures, back-calculated lengths estimated by the scale proportional hypotheses (SPH) were less than the other methods. For scales, SPH was similar to the direct proportional method of Dahl-Lea (DPM) but for cleithra and urohyal bones it was similar to BPH and Fraser-Lee. As the fish increased in age, the differences between back-calculated lengths decreased. When lengths were back-calculated using cleithra and urohyal bones, differences between the structures at age 2 years were 17 mm from DPM, 33 mm from SPH, 37 mm from BPH and 37 mm from Fraser-Lee, while at age 8 years mean differences were 7 mm from DPM and 8 mm from the SPH, BPH and Fraser-Lee methods. It is suggested that the differences would be less if: (1) back-calculated lengths are based on a large number of random samples; (2) measurements of scale radius, cleithrum length and urohyal length were precise; (3) determination of focus or origin were precise; (4) the sampling of scales were from the same row.     

Hospital Patient‐Care and Outside‐the‐Hospital Energy Profiles for Hemodialysis Services

Studies investigated the patient‐care (in‐hospital) and outside‐the‐hospital energy consumptions for delivering the hemodialysis (HD) service. A life cycle inventory methodology was used for this patient‐based analysis for two hospitals located in Wichita, Kansas. It was found that, for both hospitals, the actual HD machines consumed approximately 3.5 kilowatt‐hours (kWh) of electrical energy per HD, only 8% to 16% of the total energy used for delivering the HD service (in hospital). This increases to 9.6 to 28.9 kWh of hospital billable energy for the whole system of HD machine, auxiliaries, and dialysis water treatment. Converting these hospital direct electrical energy values to natural resource energy (nre) then adding the cradle‐to‐gate natural resource energy for the manufacturing and supply chain of all the HD consumables, the total is 78 to 149 kWh nre/HD. The nre measures all the direct fuel burned to generate energy and is thus directly related to emissions to the air, water, and land and is a direct secondary impact on public health from HD. The ratio of outside‐the‐hospital energy to direct hospital HD electrical energy consumption is 4:1 to 7:1, so a broader base exists for improvement than just the hospital.     

The clinical importance of CD4+CD7− in human diseases

The CD7 antigen is a member of the immunoglobulin superfamily that expresses on the surface of all thymocytes, a majority of mature T cells, and also natural killer cells. Interestingly, under physiological and different pathological conditions, the loss of CD7 antigen occurred in the subset of CD4⁺ memory T cells. Various functions have been proposed for CD7, including its role in the activation and intercellular adhesiveness of T cells. Several studies indicate that the number of CD4⁺CD7⁻ T cells increases in diseases such as chronic inflammation and T-cell malignancies, these being skin inflammatory lesions. Therefore, this can be useful for the diagnosis of cancer cells, especially with reference to blood origin, treatment monitoring, and establishment of new therapies. Therefore, a comprehensive review could be useful to increase our knowledge about the clinical importance of these cells in human disease.     

Possible correlation between high circulatory levels of trimethylamine-N-oxide and 2177G>C polymorphisms of hepatic flavin containing monooxygenase 3 in Kurdish Population with non-alcoholic fatty liver disease

Molecular Biology Reports - Non-alcoholic fatty liver disease (NAFLD) is a multifactorial disorder with complicated pathophysiology. Trimethylamine-N-oxide (TMAO) has been thought to be correlated...     

Apelin and stem cells: the role played in the cardiovascular system and energy metabolism

Apelin, a member of the adipokine family, is widely distributed in the body and exerts cytoprotective effects on many organs. Apelin isoforms are involved in different physiological processes, including regulation of the cardiovascular system, cardiac contractility, angiogenesis, and energy metabolism. Several investigations have been performed to study the effect of apelin on stem cell therapy. This review aims to summarize the literature representing the effects of apelin on stem cell properties. Furthermore, this review discusses the therapeutic potential of apelin‐treated stem cells for cardiovascular diseases and demonstrates the effect of stem cells overexpressing apelin on energy metabolism. Stem cells with their unique characteristics play a crucial role in the maintenance of tissue integrity. These cells participate in tissue regeneration via multiple mechanisms. Although preclinical and clinical studies have demonstrated the therapeutic potential of stem cells in various diseases, their application in regenerative medicine has not been efficient. A number of strategies such as genetic modification or treatment of stem cells with different factors have been used to improve the efficacy of cell therapy and to increase their survival after transplantation. This article reviews the effect of apelin treatment on the efficacy of cell therapy.