低氧对小鼠学习记忆力及脑中tau蛋白磷酸化的影响

目的：研究不同低氧暴露对小鼠学习记忆及脑中tau蛋白磷酸化的影响。方法：雄性昆明小鼠40只,随机分为4组（n=10）：对照组（control）、8h低氧暴露组（8h）、7d低氧暴露组（7d）和28d低氧暴露组（28d）。将低氧暴露模型组置于模拟高原海拔5500m的低压氧舱,每天低氧暴露8h,避暗和旷场实验检测其活动能力及学习记忆能力：免疫印迹技术检测小鼠海马和皮层中tau蛋白磷酸化水平。结果：随着低氧时间的增加,小鼠短期学习记忆力及活动能力下降程度增大,28d低氧暴露后其下降程度最大;海马中tau蛋白多个位点的磷酸化水平呈现升高趋势,28d时tau蛋白磷酸化程度最高（P〈0．05）;皮层中的磷酸化水平在低氧暴露7d时达到最高,低氧暴露28d时略有降低,但与control组相比仍有明显差异（P〈0．05）。结论：慢性间歇性低氧可导致小鼠学习记忆能力下降,其机制可能与tau蛋白过度磷酸化相关。     

黄原胶发酵液纯化精制研究

溶剂法直接提取的黄原胶产品含有大量菌体蛋白和色素杂质,总氮含量高、透明度差、色泽暗深。通过中性蛋白酶处理发酵液,使成品含氮量下降５６．５％;通过Ｎａ２ＳＯ３漂白液,使产品色泽大为改善。实验得出最佳酶解条件：发酵液稀释１倍,温度４４℃、ｐＨ７,加酶量１００ｕ／ｇ发酵液,作用时间２．５ｈ;最佳Ｎａ２ＳＯ３漂白条件：温度２０￣３０℃,ｐＨ５￣６,Ｎａ２ＳＯ３用量为１％（ｗ／ｗ）,作用时间１￣１．５ｈ。     

An Intermediate Category of Seed Storage Behaviour?II. EFFECTS OF PROVENANCE, IMMATURITY, AND IMBIBITION ON DESICCATION-TOLERANCE IN COFFEE

Seeds of four cultivars of arabica coffee (Coffea arabica L.)received from three continents survived desiccation to between7-2% and 11-3% moisture content (wet basis), i.e. to seed waterpotentials of –90 MPa to –150 MPa, but further desiccationreduced germination (criterion, normal seedling development)in all seed lots. Only a few individuals from four of the lotsgerminated after being dried to 4–5% moisture content.Differences in desiccation sensitivity were apparent among lotswithin each cultivar. Desiccation sensitivity in these lotswas similar to that observed in seeds of orthodox species whichhave begun to germinate. Seeds extracted from fruits of intermediatematurity (yellow) were able to tolerate greater desiccationthan those from either ripe (red) or immature (green) fruits.Imbibed storage increased desiccation sensitivity. The resultsare compatible with the view that arabica coffee seeds are unableto tolerate extreme desiccation because germination has beeninitiated before harvest.     

黄鳍金枪鱼头蛋白酶解液及分级组分对小鼠脾淋巴细胞增殖的影响

研究黄鳍金枪鱼头蛋白酶解液(EPTH)及截留相对分子量(MWCO)10、5、3和1 kD的超滤分级组分Ⅰ(10 kD)、组分Ⅱ(10～5 kD)、组分Ⅲ(5～3 kD)、组分Ⅳ(3～1 kD)和组分Ⅴ(1 kD)对小鼠脾淋巴细胞增殖的影响。采用无菌分离小鼠脾淋巴细胞,MTT法检测EPTH及各分级组分对有和没有刀豆蛋白A(ConA)、脂多糖(LPS)诱导下脾淋巴细胞增殖的影响,半微量凯氏定氮法、测定EPTH及各分级组分的氮含量。结果显示组分Ⅱ、组分Ⅴ单独作用能明显促进淋巴细胞增殖,并显著促进ConA、LPS诱导的淋巴细胞增殖;EPTH及其余3组分均没有显著的促进或抑制作用。组分Ⅱ、组分Ⅴ富集了促进小鼠脾淋巴细胞增殖的因子;组分Ⅱ、组分Ⅴ蛋白质含量分别占EPTH的38.9%和5.39%。     

水稻种子抗老化遗传分析

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