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JOHN E. CADLE HERBERT C. DESSAUER CARL GANS DONALD F. GARTSIDE 《Biological journal of the Linnean Society. Linnean Society of London》1990,40(3):293-320
Multilocus electrophoretic methods and microcomplement fixation comparisons of serum albumin are used to assess phylogenetic relationships among species of uropeltid snakes, to infer aspects of their population biology and biogeography, and to evaluate their relationships to other primitive snakes (Henophidia). There is very good agreement between phylogenetic inferences derived from the electrophoretic data and those derived from the albumin immunological data. Protein variation detected by electrophoresis is relatively high among 17 operational taxonomic units (OTUs) examined. The mean number of alleles per locus (5.1 across all OTUs), levels of polymorphism (25% of loci), and heterozygosity (4–6%), are typical of, or greater than, values reported for other snakes. Species of uropeltids are genetically highly differentiated, as measured by genetic distances (lowest interspecific Nei's unbiased genetic distances, 0.22-0.27 among several Sri Lankan species; 2.3 between Teretrurus of India and other uropeltines). The phylogenetic tree most consistent with both the immunological and electrophoretic data shows uropeltines from Sri Lanka to be monophyletic, but the Indian species are paraphyletic with respect to those from Sri Lanka. Rhinophis travancoricus of India is inferred to be the sister taxon to the Sri Lankan radiation. As the genera are presently understood, neither Rhinophis nor Uropeltis appears to be monophyletic. A biogeographic scenario derived from the phylogenetic hypothesis suggests an early diversification of uropeltids in India, followed by a single invasion into the lowlands of Sri Lanka. Subsequent evolution on Sri Lanka resulted in occupation of montane biotopes. Cylindrophis is the sister group to uropeltines and is considered a member of the Uropeltidae. The immunological data indicate no phylogenetic association between uropeltids and other ‘anilioid’ taxa, specifically Anilius, Loxocemus or Xenopeltis, although we cannot rule out a very remote relationship. We specifically reject the hypothesis that uropeltines and scolecophidians form a clade relative to henophidians. High levels of genetic variation and a trend toward negative FIS values for polymorphic loci in three populations suggest generally large effective population sizes and outbreeding in these species. The niche-width variation hypothesis for allozyme loci is not supported by the uropeltid data. In comparison to other vertebrates, the relationship between Nei's genetic distance and albumin immunological distance in uropeltids suggests either conservative albumin evolution or strong differentiation at electrophoretic loci. 相似文献
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ROSE MARIE. PANGBORN JEAN-XAVIER. GUINARD HERBERT L. MEISELMAN 《Journal of sensory studies》1989,4(1):31-53
Perceived intensity of bitterness of caffeine (0.5 - 1.6 g/100 mL) and of quinine HCI (0.5 - 1.6 g/L) added to hot cocoa gave similar response distributions with concentration by category scales (CS) and magnitude estimation (ME). Using caffeine additives, CS and graphic analogue scales (GS) gave similar bitterness response functions. Parabolic functions provided the best fit between concentration and ratings for CS, GS and ME. The data demonstrated no difference for ME of bitterness intensity between free or fixed moduli. Hedonic responses also were not modified by whether the modulus was free or fixed, however, greater ME values were ascribed to lower concentrations in the series when the reference was 4 g/L than when it was 2 g/L caffeine. CS intensity response distributions were similar between a series with a larger number of low levels versus a series with a larger number of high levels across the same concentration range. The results indicate that ME may be inappropriate for scaling of degree of liking, independent of type of modulus. 相似文献
24.
ADHESION IN BYSSALLY ATTACHED BIVALVES 总被引:2,自引:0,他引:2
J. HERBERT WAITE 《Biological reviews of the Cambridge Philosophical Society》1983,58(2):209-231
The byssus is a structure produced by marine bivalve molluscs to adhere, usually permanently, to substrata under water. As the adhesion of synthetic polymers to surfaces is predictably compromised by the presence of water, particularly bulk water, it is of particular interest to discover the mechanism of byssal adhesion. In most species, the byssus consists of at least four essential components: acid mucopolysaccharides, adhesive protein, fibrous proteins, and an oxidative enzyme, polyphenoloxidase. The function of the mucopolysaccharide component is still uncertain, but it can conceivably be used by the animal as a temporary adhesive, a surface modifying agent, and/or a stabilizing filler for the permanent adhesive. The adhesive protein known as the polyphenolic protein in Mytilus is but a thin plaque applied to the substrate surface by the foot of the animal. The molecular and physical properties of this adhesive protein conform remarkably well to what one expects of an ideal synthetic polymer, i.e. high molecular weight, abundance of large and polar side chains, near-zero surface contact angle, and total water-insolubility after setting. The fibrous proteins constitute the major portion of the thread or ribbon-like material connecting the animal to the adhesive plaque on the substrate surface. These proteins are packed in ordered crystalline arrays, e.g. β-pleated sheet and collagen helix (in mytilids) as is to be expected from structural tensile elements of Nature. The enzyme polyphenoloxidase is presumed to induce intermolecular cross-linking of proteins in the fibrous and adhesive portions of the byssus. In Mytilus the natural substrates of the enzymc may be the dopa-containing polyphenolic protein and accessory gland protein. 相似文献
25.
SYNOPSIS. The influence of the type of growth carbohydrate on the subsequent metabolic activity of Trichomonas gallinae was investigated. Washed suspensions of cells collected from CPL-glucose, CPL-maltose, CPL-galactose, and CPL-glucose-maltose media were examined in the Warburg respirometer for their ability to utilize glucose, maltose, and galactose. Comparisons of the metabolic parameters of substrate consumption, changes in glycogen content, and CO2 and H2 production were made. The pattern of utilization of the sugars, both qualitatively and quantitatively, depended upon the type of carbohydrate in the CPL medium used to culture the cells and upon the time of exposure of the cells to a particular sugar in the medium. 相似文献
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Meiotic Protein in Spermatocytes of Mammals 总被引:8,自引:0,他引:8
THE DNA-binding protein in meiotic cells of Lilium1 has a very high binding affinity for single stranded DNA and also the unusual property of catalysing the renaturation of thermally denatured lily DNA at room temperature. Significantly, these and other in vitro properties are very similar to those of the “gene 32-protein” which is essential to genetic recombination in T4 bacteriophage2 and the possibility that this protein may have a function in meiotic recombination of Lilium led us to a more extensive study of its behaviour3. 相似文献
28.
The Foraging Ecology of Migratory Shorebirds in Marine Soft-sediment Communities: The Effects of Episodic Predation on Prey Populations 总被引:1,自引:0,他引:1
This paper explores the extent to which life histories of infaunalprey have been molded and maintained by predation. It is arguedsuch relationships should be most evident for episodic predation,where the predation is predictable, intense and short-lived.Migratory shorebirds are used herein as model episodic predators.Four stop-over areas in North America, for which experimentaldata exist, are compared. Evidence exists that SemipalmatedSandpipers in the upper Bay of Fundy maintain the observed lifehistory of their major prey, the amphipod Corophium volutator.In southeastern Massachusetts, shorebirds significantly reducethe abundance of their prey but in a frequency-dependent fashion,precluding life history responses of the prey. For Grays Harbor,Washington and Delaware Bay, there is no detectable effect ofshorebird predation on the infauna. In three of the four studies,there is no apparent effect of early shorebird migrants in significantlyincreasing the required stop-over of later migrants by depressionof prey densities. The data support the characterization ofsoft-sediment communities as loosely organized assemblages whosespecific constitution is determined more by ecological combiningability than by specific evolutionary responses to associatedspecies. 相似文献
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