响应面法优化北五味子褐斑病内生生防真菌淡紫拟青霉WG9发酵工艺及发酵产物稳定性研究

为提高北五味子内生生防真菌WG9发酵液对褐斑病病原菌的抑菌率,本研究采用单因素实验及响应面法对其发酵条件进行优化.首先采用单因素实验,以发酵时间(h)、发酵温度(℃)、摇床转速(r/min)及初始pH为考察指标,测定对病原菌的抑菌率及生防真菌WG9的菌丝体生物量,以获取单因素实验的最优值,然后使用Box-Behnken...     

捕食线虫丝孢菌的菌寄生性测定

研究了节丛孢Arthrobotrys、单顶孢Monacrosporium和隔指孢Dactylella三个捕食线虫丝孢菌属16个菌株,对水稻立枯丝核菌RhizoctoniasolaniAG1、大豆核盘菌Sclerotiniasclerotiorum、茄科镰刀菌Fusariumsolani和恶疫霉Phytophthoracactorum四种常见土壤植物病原真菌的菌寄生性。结果表明供试菌可以通过弹簧式菌丝圈缠绕、类附着胞结构吸附、简单的菌丝缠绕或者贴附寄主菌丝生长四种方式寄生病原菌。其中,绝大多数菌株对立枯丝核病菌有寄生作用,一些供试真菌对其它三种病原真菌有寄生现象。利用孢子液浸泡法测定了其中5种捕食线虫真菌对核盘菌菌核的寄生能力,显示有较高寄生率。     

吡咯喹啉醌对冬小麦某些生理特性和穗部性状的影响(简报)

冬小麦孕穗期叶面喷吡咯喹啉醌(PQQ)后,小麦叶片中叶绿素含量、叶片的光合速率、硝酸还原酶和谷丙转氨酶的活性均提高,穗部小花败育减少,结实率提高。采用的3种浓度PQQ(50、100、170μmol·L     

Effects of Growth Retardants on the Growth of Excised Roots of Dolichos lablab L. in Culture

The effects of various growth-retarding chemicals on the growthof excised roots of Dolichos lablab in sterile culture are investigated.Application of a range of concentrations of CCC, phosfon, andB-995 inhibited growth in length of the roots by reducing thefrequency of cell division, with little or no effect on cellelongation. Treated roots had generally lower DNA, RNA, andprotein contents than controls, although CCC-treatment significantlyenhanced the soluble nitrogen content of the roots. The inhibitoryeffects of growth retardants were not reversed by IAA or GA,but substances like choline chloride and pyridoxine hydrochloridewere partially effective in reinstating normal growth in rootsgrowing in concentrations of CCC producing about 50 percentinhibition of growth in length.     

Chromosome variation in the genus Pinellia (Araceae) in China and Japan

The chromosome numbers of 23 populations belonging to seven species of Pinellia Tenore were counted. The basic chromosome number of all the species studied was x  = 13, and the previously reported basic numbers x  = 9 and x  = 14 in P. ternata and P. cordata were not confirmed. Chromosome numbers of 2 n  = 26 in P. polyphylla and 2 n  = 78 in P. integrifolia are reported for the first time. Heptaploid (7 x ) and nonuploid (9 x ) are new ploidy levels reported for P. ternata , and hexaploid for P. cordata. Taxonomic, phylogenetic and phytogeographical inferences are made for the genus. Particular attention was given to P. ternata , the most widely distributed species in the genus, and considerable variation of chromosome number was found in its different populations. Based on chromosome studies of 11 populations of P. ternata , together with 12 populations reported in previous studies, the lower reaches of the Yangze River are identified as its centre of origin. From there it dispersed, with generation of hexaploid, heptaploid, octoploid and nonuploid forms.  © 2005 The Linnean Society of London, Botanical Journal of the Linnean Society , 2005, 147 , 449–455.     

上海市南汇东滩围垦后海岸带湿地冬春季水鸟生境选择

2009年12月-2010年5月,对上海市南汇东滩围垦后城市湖泊湿地、实验修复湿地、抛荒湿地等三类人工湿地冬季雁鸭类群落和春季鸻鹬类群落展开调查,并对雁鸭类和鸻鹬类群落特征与相应生境因子进行相关分析.结果显示:三类湿地雁鸭类的种类无显著差异,但密度有显著差异:实验修复湿地是抛荒湿地的3.77倍,是城市湖泊湿地的6.03倍.鸻鹬类种类和密度:实验修复湿地分别是抛荒湿地的2.88和5.70倍.两者种类和密度差异均极显著.雁鸭类种类、密度、Shannon-Wiener多样性指数(H')与明水面面积显著正相关;种类、H'与植被面积显著负相关;种类、密度、H'均匀度与植被盖度显著负相关;H'与平均水位显著正相关.鸻鹬类种类、密度均与裸露浅滩面积显著正相关.建议对已围垦土地的利用以大中型鱼蟹塘、水田为主,以提供足够的明水面给越冬的雁鸭类栖息.通过割除植被等方法在春季提供一定的裸露浅滩面积供迁徙的鸻鹬类栖息.     

Efficacy and safety of mesenchymal stromal cell treatment from related donors for patients with refractory aplastic anemia

Background aimsThis study evaluated the feasibility, safety and immunological effects of the intravenous administration of mesenchymal stromal cells (MSCs) from a related donor in patients with refractory aplastic anemia (AA).MethodsA mean of 6 × 10⁵/kg (range, 5.0–7.1 × 10⁵) MSCs were injected intravenously to 18 patients, including 14 patients with nonsevere AA and four patients with severe AA who were refractory to prior immunosuppressive treatment. The outcomes of patients treated with MSCs were evaluated and compared with a historic control cohort, including 18 patients with refractory AA.ResultsTwo patients had injection-related adverse events, including transient fever and headache. No major adverse events were reported during the follow-up period. An immunological analysis revealed an increased proportion of CD4⁺CD25⁺ FOXP3⁺regulatory T cells in peripheral mononuclear cells. Following up for 1 year, six of 18 patients (33.3%) achieved a complete response or a partial response to MSC treatment. In six patients, two achieved a complete response including a recovery of three hematopoietic cell lines after MSCs therapy at days 88 and 92, two patients achieved only a red cell recovery with hemoglobin levels >100 g/L at days 30 and 48 and two patients had only a platelet recovery with a platelet count of >60 × 10⁹/L at days 54 and 81. In the control cohort, only one patient (5.56%) achieved a partial response during the follow-up period.ConclusionsThe data from the present study suggest that treatment with MSCs from a related donor may be a promising therapeutic strategy for patients with refractory AA. The trial has been registered at ClinicalTrials.gov: identifier NCT01305694.     

Synthesis,biological evaluation,and molecular docking studies of novel 1,3,4-oxadiazole derivatives possessing benzotriazole moiety as FAK inhibitors with anticancer activity

1,3,4-Oxadiazole derivatives have drawn continuing interest over the years because of their varied biological activities. In order to search for novel anticancer agents, we designed and synthesized a series of new 1,3,4-oxadiazole derivatives containing benzotriazole moiety as potential focal adhesion kinase (FAK) inhibitors. All the synthesized compounds were firstly reported. Among the compounds, compound 4 shows the most potent inhibitory activity against MCF-7 and HT29 cell lines with IC₅₀ values of 5.68 μg/ml and 10.21 μg/ml, respectively. Besides, all the compounds were assayed for FAK inhibitory activity using the TRAP–PCR–ELISA assay. The results showed compound 4 exhibited the most potent FAK inhibitory activity with IC₅₀ values of 1.2 ± 0.3 μM. Docking simulation by positioning compound 4 into the FAK structure active site was performed to explore the possible binding mode. Apoptosis which was analyzed by flow cytometry, demonstrated that compound 4 induced apoptosis against MCF-7 cells. Therefore, compound 4 may be a potential anticancer agent against MCF-7 cancer cell.     

Effects of leaf shape plasticity on leaf surface temperature北大核心CSCD

Aims The shape plasticity of plant leaves is an important survival strategy to high temperature and drought in arid region, yet reliable evidences are insufficient to validate the fundamental concepts. Our objective was to demonstrate the specific effects of leaf morphology on leaf surface temperature. Methods Infrared thermal images were processed to determine the leaf temperature and shape parameters of simulated and actual leaf shape. Microclimatic conditions were recorded using a automatic weather station near the sampling plot, including wind speed, radiation and air temperature. Important findings Under the drought and high temperature, the plasticity of leaf shape appeared an important measure to regulate leaf temperature, except leaf transpiration. The exchange rates of matter and energy between leaves and the environment were enhanced by smaller leaves that effectively decreased leaf temperature. With low wind speed and high temperature, leaf surface temperature decreased 2.1 °C per 1 cm reduction in leaf width. However, leaf surface temperature of a simulated leaf decreased 0.60–0.86 °C per 1 cm reduction in leaf width. Results from this study will help us to understand plant adaptability and survival strategy in arid region. © 2018 Editorial Office of Chinese Journal of Plant Ecology. All rights reserved.