Studies in the Physiology of the Onion Plant: VI. A SENSITIVE MORPHOLOGICAL TEST FOR BULBING AND ITS USE IN DETECTING BULB DEVELOPMENT IN STERILE CULTURE

Bulb development in the onion plant, which occurs in responseto the stimulus of long days, has two main morphological features:swelling of the base of the pseudostem and the formation ofscales from leaf initials produced at the stem apex. The swellingmay be expressed as the ‘bulbing ratio’, i.e. theratio of the maximum diameter at the base to the minimum atthe neck, but this is a somewhat insensitive measure wider adverselight conditions and no ratio can be specified at which bulbingis first apparent. In the absence of bulbing, leaf initialshave at all stages of development a blade longer than the leafsheath, giving a ‘leaf ratio’ of blade/sheath atall times greater than unity. In scale formation, when bulbdevelopment begins, the sheath of a small initial starts togrow sooner than in foliage leaf formation and blade growthis suppressed; the leaf ratio therefore falls rapidly belowunity and this is diagnostic of bulbing. Characteristic graphsof leaf ratio plotted against leaf number from the apex demonstratethe stage of bulbing reached and a fall in leaf ratio from nearthe apex in such a graph can indicate the beginning of bulbingbefore any actual scales (with ratios less than unity) havebeen formed. In normally grown plants, differentiation intofoliage leaves or scales appears to take place when the initialsreach about 1 mm in length. When the incidence of bulbing within a treatment is very variable,difficulties arise in averaging the leaf ratio curves for thedifferent plants. Mean number of scales per plant, or mean minimumleaf ratio, may then be used as measures of bulbing. Heavy shading in winter was found to delay but not prevent thebulbing of those plants which survived. ‘Buds’, consisting of a portion of stem with theapex surrounded by leaf initials, but no scales, were dissectedout from onion sets and cultured aseptically in tubes. Limitedgrowth was obtained and rooting occurred in some cases. In thepresence of sucrose, swelling of leaf sheaths and reductionof the leaf ratios took place; these responses increased withsucrose concentration. Glucose similarly stimulated bulbingbut high osmotic pressure caused by mannitol or mineral nutrientshad no effect. Rooting appeared to reduce the degree of bulbing,possibly by competing for carbohydrate supply. The bulb developmentin these sterile cultures was most unusual in occurring in shortphotoperiods and the possibility of a residue of ‘bulbinghormone’ being stored in sets is discussed.     

Evaluation of non-conventional treatments for control of the biting louse (Bovicola ovis) on sheep

variety of non-conventional treatments was applied to biting louse ( Bovicola ovis ) infested sheep in order to evaluate ways in which farmers could control the louse infestations and still maintain Organic Production Standards.
In one trial, louse scores of sheep shorn but kept dry or wetted by water alone or with water plus detergent were compared with unshorn sheep treated similarly. Shearing alone accounted for a 35.7–66.3% reduction in mean louse scores. Wetting alone either with water or with water and added detergent accounted for a 26.9–35.3% reduction in mean louse scores. The combined effects on mean louse scores of shearing and wetting, as opposed to shearing alone, were statistically significant on two of the three farms at 32–35 days post-treatment. The effects persisted for the duration of the trial (between 48 and 52 days), at which point shearing and wetting with detergent provided 95.3–99.6% control of lice. In a second trial, a range of insecticidal substances considered acceptable by Organic Production Standards, azadirachtin (neem), pyrethrum, soap, was applied to louse-infested sheep and their efficacy compared with that of a commercial formulation of cypermethrin. The sheep treated with azadirachtin and pyrethrum had significantly fewer lice than either the control or soap treated sheep over the 48 days of the trial. Neither azadirachtin nor pyrethrum were significantly less effective than cypermethrin. Control (reduction in louse score) of 85.0–100% was achieved over the period of the trial.
It is concluded that most of the non-conventional treatments evaluated had a useful and cost-effective role to play in reducing louse numbers on sheep for at least 40–50 days. The lack of persistence compared with that obtained with conventional insecticides was the only apparent drawback.     

Ten polymorphic microsatellite markers in the invasive round goby (Neogobius melanostomus) and cross‐species amplification

Ten tetranucleotide, dinucleotide and compound microsatellite loci were isolated and characterized for the round goby, Neogobius melanostomus. Analysis of 64 gobies from one nonindigenous population in Lake Erie, Ontario, Canada, indicated that allele number varied from three to 12 per locus, while observed heterozygosity ranged between 0.33 and 0.86. Eight of these primers showed some amplification in other species in four genera. These newly developed microsatellite markers are a powerful tool that will provide insights into population structure and dispersal of the round goby in their novel environment.     

Characterization of eight polymorphic microsatellite DNA markers for the greenside darter,Etheostoma blennioides (Percidae)

The greenside darter, Etheostoma blennioides is a small benthic fish found in fast‐flowing streams in eastern North America. In Canada, this species is native to three, and introduced into one, Great Lakes tributaries in southwestern Ontario. It is currently listed as a species of Special Concern. To characterize population genetic structure and diversity in the Canadian populations of greenside darter, eight polymorphic microsatellite markers were developed for the species. The polymerase chain reaction primers were tested between 32 and 60 individuals from the Sydenham River and yielded a high number of alleles (four to 42 per locus), and observed heterozygosities ranging from 0.14 to 0.82.     

Neither compatible nor self-incompatible pollinations of Brassica napus involve reorganization of the papillar cytoskeleton

Compatible pollination of Brassica napus necessitates pollen hydration, pollen germination and growth of the pollen tube through the loosened walls of stigmatic papillar cells, whereas self-incompatible (SI) pollinations fail at one of these stages. Analyses of the early stages of pollination show that at high (but not low) relative humidities, both compatible and SI pollen hydrates, but SI germination is reduced and the rare pollen tubes generally fail to penetrate the papillar walls, although there is some wall loosening. Inside the papillae, both compatible and SI interactions may induce the formation of callose, but there is no evidence for a major accumulation of cytoplasm or secretory vesicles in the vicinity of the pollen tubes and neither microtubule nor F-actin patterns re-arrange in this zone. These observations indicate that the source of the wall-loosening enzymes is probably the pollen tube or pollen coat, and that the common cellular responses of plants to attempted invasions have become suppressed in the papilla–pollen tube interaction.     

Compensation Points and Carbon Dioxide Enrichment for Lettuce Grown Under Glass in Winter

Measurements of , the minimum intercellular-space carbon dioxideconcentration or carbon dioxide compensation point, made attwo temperatures and at light intensities from 15 to 1,500 f.c.,were used to study the relation between the compensation pointsfor light and carbon dioxide. This was found to be hyperbolicand consistent with the Warburg–Maskell model for photosynthesis.The apparently beneficial effects of carbon dioxide enrichmentof the atmosphere at increased temperature, found in practicewith lettuce or other crops grown under glass at light intensitiesthat might be expected to be severely limiting, must be in partdue to the reduction of the light compensation point at highcarbon dioxide concentration.     

The distribution and orientation of epizoic barnacles on crabs

The distribution and orientation of Balanus crenatus on Carcinus maenas is described. Elminius modestus was also found on C. maenas but it was not common. Both species of barnacle were also found on Cancer pagurus, although neither species of barnacle appeared to be as common on Cancer as on Carcinus. Possible reasons for this are discussed.
Treating the carapaces of similar sized C. maenas as sampling units it is clear that the distribution of B. crenatus over these sampling units is not random but is aggregated. This probably arises because of the gregarious nature of the cyprids when they settle out but other possibilities are considered. B. crenatus on the carapace of C. maenas is found exclusively in the grooves and depressions on the carapace and this is because the cyprids preferentially settle in concavities.
The orientation of B. crenatus also shows a consistent pattern, with the cirral nets facing predominantly backwards. This pattern could arise because the barnacle cyprids orientate to water currents, generated by the exhalant respiratory currents, flowing forwards over the carapace.     

Mutualism variation in the nodulation response to nitrate

The evolution of mutualisms under novel selective pressures will play a key role in ecosystem responses to environmental change. Because fixed nitrogen is traded in plant–rhizobium mutualisms, increasing N availability in the soil is predicted to alter coevolution of these interactions. Legumes typically decrease the number of associations (nodules) with rhizobia in response to nitrate, but the evolutionary dynamics of this response remain unknown. We grew plant and rhizobium genotype combinations in three N environments to assess the coevolutionary potential of the nodule nitrate response in natural communities of plants and rhizobia. We found evidence for coevolutionary genetic variation for nodulation in response to nitrate (G × G × E interaction), suggesting that the mutualism response to N deposition will depend on the combination of partner genotypes. Thus, the nitrate response is not a fixed mechanism in plant–rhizobium symbioses, but instead is potentially subject to natural selection and dynamic coevolution.