全文获取类型
收费全文 | 36806篇 |
免费 | 3145篇 |
国内免费 | 6129篇 |
出版年
2024年 | 167篇 |
2023年 | 671篇 |
2022年 | 1429篇 |
2021年 | 2113篇 |
2020年 | 1506篇 |
2019年 | 1930篇 |
2018年 | 1668篇 |
2017年 | 1271篇 |
2016年 | 1680篇 |
2015年 | 2291篇 |
2014年 | 2818篇 |
2013年 | 3031篇 |
2012年 | 3385篇 |
2011年 | 3147篇 |
2010年 | 2011篇 |
2009年 | 1942篇 |
2008年 | 2215篇 |
2007年 | 1973篇 |
2006年 | 1686篇 |
2005年 | 1369篇 |
2004年 | 1114篇 |
2003年 | 993篇 |
2002年 | 803篇 |
2001年 | 764篇 |
2000年 | 716篇 |
1999年 | 612篇 |
1998年 | 355篇 |
1997年 | 320篇 |
1996年 | 301篇 |
1995年 | 268篇 |
1994年 | 246篇 |
1993年 | 177篇 |
1992年 | 230篇 |
1991年 | 195篇 |
1990年 | 147篇 |
1989年 | 109篇 |
1988年 | 88篇 |
1987年 | 82篇 |
1986年 | 55篇 |
1985年 | 60篇 |
1984年 | 30篇 |
1983年 | 34篇 |
1982年 | 32篇 |
1981年 | 26篇 |
1980年 | 8篇 |
1979年 | 6篇 |
1976年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1950年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 32 毫秒
151.
温度对黄粉虫成虫繁殖的影响 总被引:2,自引:0,他引:2
黄粉虫是多种小型经济动物的常用优良饵料,本试验在20.1℃,24.0℃、28.5℃、31.7℃和36.5五种恒温下饲养该成虫结果,成虫寿命平均分别为63.0、54.2、38.8、38.0和26.1天;每雌平均产卵量则分别为200.3、207.3、122.3、115.8和81.2粒,成虫平均生产1g卵消耗麸皮量分别为2.66、2.00、2.19、2.14和4.15g。结果说明人工繁殖黄粉虫的成虫期温度以24℃为最适宜。 相似文献
152.
The competition method in which the Fenton reaction is employed as an OH radical generator and deoxyribose as a detecting molecule, has been used to determine the rate constants for reactions of the OH radical with its scavengers. Nonlinear competition plots were obtained for those scavengers which reacted with the Fenton reagents (Fe2+ or H2O2). Ascorbic acid is believed to overcome this problem. We have investigated the kinetics of deoxyribose degradation by -OH radicals generated by the Fenton reaction in the presence of ascorbic acid, and observed that the inclusion of ascorbic acid in the Fenton system greatly increased the rate of OH radical generation. As a result, the interaction between some scavengers and the Fenton reagents became negligeable and linear competition plots of A7A vs scavenger concentrations were obtained. The effects of experimental conditions such as, the concentrations of ascorbic acid, deoxyribose, H2O2 and Fe2+-EDTA, the EDTA/Fe2+ ratio as well as the incubation time, on the deoxyribose degradation and the determination of the rate constant for mercaptoethanol chosen as a reference compound were studied. The small standard error, (6.76± 0.21) ±' 109M-1s-1 observed for the rate constant values for mercaptoethanol determined under 13 different experimental conditions, indicates the latter did not influence the rate constant determination. This is in fact assured by introducing a term, kx, into the kinetic equation. This term represents the rate of-OH reactions with other reagents such as ascorbic acid, Fe2+-EDTA, H2O2 etc. The agreement of the rate constants obtained in this work with that determined by pulse radiolysis techniques for cysteine, thiourea and many other scavengers, suggests that this simple competition method is applicable to a wide range of compounds, including those which react with the Fenton reagents and those whose solubility in water is low. 相似文献
153.
Na2SO3对热-DTT活化的游离CF1及类囊体膜上CF1-ATPase活力均有显著的促进作用,NaHCO3亦有明显的促进作用。Na2SO3和NaHCO3的促进作用与它们解除Mg2+的抑制作用有关。从NaHCO3和Na2SO3及它们与Mg2+之间的竞争性关系,表明三者是结合在酶的同一部位上。Na2SO3可明显降低热-DTT活化的游禹CF1-ATPase催化反应的活化能,这可能与促进产物ADP的释放有关。 相似文献
154.
155.
The reductive acetyl coenzyme A pathway: sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum. 总被引:1,自引:1,他引:0 下载免费PDF全文
The methyltransferase (MeTr) from Clostridium thermoaceticum transfers the N5-methyl group of (6S)-methyltetrahydrofolate to the cobalt center of a corrinoid/iron-sulfur protein in the acetyl coenzyme A pathway. MeTr was purified to homogeneity and shown to lack metals. The acsE gene encoding MeTr was sequenced and actively expressed in Escherichia coli at a level of 9% of cell protein. Regions in the sequence of MeTr and the E. coli cobalamin-dependent methionine synthase were found to share significant homology, suggesting that they may represent tetrahydrofolate-binding domains. 相似文献
156.
Characterization of the interaction of the glp repressor of Escherichia coli K-12 with single and tandem glp operator variants. 总被引:2,自引:2,他引:0 下载免费PDF全文
N Zhao W Oh D Trybul K S Thrasher T J Kingsbury T J Larson 《Journal of bacteriology》1994,176(8):2393-2397
The glp operons of Escherichia coli are negatively controlled by the glp repressor. Comparison of the repressor-binding affinities for consensus and altered consensus operators in vivo showed that all base substitutions at positions 3, 4, 5, and 8 from the center of the palindromic operator caused a striking decrease in repressor binding. Substitutions at other positions had a severe to no effect on repressor binding, depending on the base substitution. The results obtained indicate that the repressor binds with highest affinity to operators with the half-site WATKYTCGWW, where W is A or T, K is G or T, and Y is C or T. Strong cooperative binding of the repressor to tandem operators was demonstrated in vivo. Cooperativity was maximal when two 20-bp operators were directly repeated or when 2 bp separated the two operators. Cooperativity decreased with the deletion of 2 bp or the addition of 4 bp between the individual operators. Cooperativity was eliminated with a 6-bp insertion between the operators. 相似文献
157.
Recurrent response patterns of a zooplankton community to whole-lake fish manipulation 总被引:2,自引:0,他引:2
XI HE ‡ MARK D. SCHEURELL PATRICIA A. SORANNO RUSSELL A. WRIGHT † 《Freshwater Biology》1994,32(1):61-72
1. In a series of whole-lake manipulations conducted from 1984 to 1991, planktivorous fishes were alternately removed and restocked in a small mesotrophic lake, resulting in dramatic changes in the zooplankton community. 2. Response patterns in the zooplankton community, which include species and size structure, and within-year community variability, were examined. Variation in the zooplankton community in unmanipulated years was much lower than that in manipulated years, regardless of direction of the manipulation (i.e. decreasing or increasing planktivory). 3. The succession of zooplankton species abundance was repeated in the second removal of planktivorous fishes. The community shifted from small-bodied cladocerans, copepods and rotifers, through an intermediate state with high abundance of Holopedium, to an assemblage dominated by large-bodied daphnids. 相似文献
158.
Ten soils collected from the major arable areas in Britain were used to assess the availability of soil sulphur (S) to spring
wheat in a pot experiment. Soils were extracted with various reagents and the extractable inorganic SO4-S and total soluble S(SO4-S plus a fraction of organic S) were determined using ion chromatography (IC) or inductively-coupled plasma atomic emission
spectrometry (ICP-AES), respectively. Water, 0.016 M KH2PO4, 0.01 M CaCl2 and 0.01 M Ca(H2PO4)2 extracted similar amounts of SO4-S, as measured by IC, which were consistently smaller than the total extractable S as measured by ICP-AES. The amounts of
organic S extracted varied widely between different extractants, with 0.5 M NaHCO3 (pH 8.5) giving the largest amounts and 0.01 M CaCl2 the least. Organic S accounted for approximately 30–60% of total S extracted with 0.016 M KH2PO4 and the organic C:S ratios in this extract varied typically between 50 and 70. The concentrations of this S fraction decreased
in all soils without added S after two months growth of spring wheat, indicating a release of organic S through mineralisation.
All methods tested except 0.5 M NaHCO3-ICP-AES produced satisfactory results in the regression with plant dry matter response and S uptake in the pot experiment.
In general, 0.016 M KH2PO4 appeared to be the best extractant and this extraction followed by ICP-AES determination was considered to be a good method
to standardise on. 相似文献
159.
160.