全文获取类型
收费全文 | 759683篇 |
免费 | 86768篇 |
国内免费 | 375篇 |
专业分类
846826篇 |
出版年
2016年 | 8385篇 |
2015年 | 11543篇 |
2014年 | 13511篇 |
2013年 | 20006篇 |
2012年 | 21849篇 |
2011年 | 22480篇 |
2010年 | 15270篇 |
2009年 | 14081篇 |
2008年 | 19970篇 |
2007年 | 20878篇 |
2006年 | 19551篇 |
2005年 | 18795篇 |
2004年 | 18537篇 |
2003年 | 18192篇 |
2002年 | 17724篇 |
2001年 | 32583篇 |
2000年 | 32824篇 |
1999年 | 26212篇 |
1998年 | 9431篇 |
1997年 | 10181篇 |
1996年 | 9591篇 |
1995年 | 9052篇 |
1994年 | 8810篇 |
1993年 | 9009篇 |
1992年 | 22705篇 |
1991年 | 22454篇 |
1990年 | 21915篇 |
1989年 | 21628篇 |
1988年 | 20050篇 |
1987年 | 19139篇 |
1986年 | 17852篇 |
1985年 | 18305篇 |
1984年 | 15265篇 |
1983年 | 13178篇 |
1982年 | 10192篇 |
1981年 | 9103篇 |
1980年 | 8675篇 |
1979年 | 14606篇 |
1978年 | 11468篇 |
1977年 | 10675篇 |
1976年 | 10176篇 |
1975年 | 11069篇 |
1974年 | 11809篇 |
1973年 | 11664篇 |
1972年 | 10725篇 |
1971年 | 9759篇 |
1970年 | 8329篇 |
1969年 | 8143篇 |
1968年 | 7462篇 |
1967年 | 6553篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
901.
Divergence time estimates of mammals from molecular clocks and fossils: Relevance of new fossil finds from India 总被引:1,自引:0,他引:1
G. V. R. Prasad 《Journal of biosciences》2009,34(5):649-659
This paper presents a brief review of recent advances in the classification of mammals at higher levels using fossils and
molecular clocks. It also discusses latest fossil discoveries from the Cretaceous — Eocene (66–55 m.y.) rocks of India and
their relevance to our current understanding of placental mammal origins and diversifications. 相似文献
902.
903.
The Capricorn silvereye (Zosterops lateralis chlorocephalus) is ideally suited to investigating the genetic basis of body size evolution. We have isolated and characterized a set of microsatellite markers for this species. Seven out of 11 loci were polymorphic. The number of alleles detected ranged from two to five and observed heterozygosities between 0.12 and 0.67. One locus, ZL49, was found to be sex‐linked. This moderate level of diversity is consistent with that expected in an isolated, island population. 相似文献
904.
D. L. Grekov 《Plasma Physics Reports》2001,27(12):1011-1016
A possible way to affect the influxes of heavy impurities into the plasma of a stellarator with poloidal slits in the vacuum wall (e.g., the W7-AS stellarator) by RF heating of the impurities is discussed. It is shown that the influxes can be reversed in direction by applying a relatively low RF power. The design features of the W7-AS stellarator are such that there is no need to place additional antennas inside the vacuum chamber. 相似文献
905.
906.
In an ultrastructural study of the hepatopancreas of Porcellio scaber, an obligate intracellular parasite, Chlamydia, was noted in the epithelial cells. Although the infection was found to extend the entire length of the hepatopancreas, it was most extensive in the glandular region. Indirect immunofluorescence testing revealed no cross-reactivity with either lymphogranuloma venereum or psittacosis antisera. 相似文献
907.
A human NK and K cell subset shares with cytotoxic T cells expression of the antigen recognized by antibody OKT8 总被引:18,自引:0,他引:18
B Perussia V Fanning G Trinchieri 《Journal of immunology (Baltimore, Md. : 1950)》1983,131(1):223-231
The antigen recognized by monoclonal antibody OKT8 is expressed on the cell membrane of 30 to 50% of human NK/K cells. The reactivity of OKT8 with NK/K cells was determined by indirect methods (treatment of the effector cells with OKT8 antibody and complement (C) and separation of OKT8(+) and (-) effector cell populations by fluorescence-activated cell sorting or by rosetting techniques) and, at single cell level, by C-dependent lysis of effector NK cells that bind and kill K562 targets. Analysis by indirect immunofluorescence (flow cytofluorometry) of lymphocyte subpopulations mediating NK/K cytotoxic activity and deprived of OKT8(+) T cells reveals that the NK/K cell subset bears OKT8 antigen at a density lower than that present on cytotoxic T cells. The OKT8 antigen on NK/K cells is trypsin- and pronase-sensitive, but it is resynthesized by the same effector cells during 24 hr of culture at 37 degrees C. OKT8 antibody does not inhibit NK killing, and, on a per cell basis, OKT8(+) cells within the NK/K subset mediate the same level of cytotoxic activity as OKT8(-) NK/K cells. Analogous results were obtained by using anti-Leu-2a, an antibody with the same specificity as OKT8 on cytotoxic/suppressor T cells, but not when OKT5 was used, which might identify a distinct epitope on the same antigenic molecule. The possible significance of these findings in understanding the cell lineage of NK/K cells is discussed. 相似文献
908.
Dependence of cytoplasmic calcium transients on the membrane potential in isolated nerve endings of the guinea pig 总被引:5,自引:0,他引:5
The relation of changes in internal, free Ca2+, measured with arsenazo III, to the membrane potential, measured with the cyanine dye di-S-C2(5) or 86Rb+ distribution ratio, was studied in isolated guinea pig cortical nerve endings. Depolarization of the plasma membrane with veratridine or gramicidin as well as addition of ionophore A23187 led to an increase in cytosolic Ca2+. Only the response to veratridine was inhibited by tetrodotoxin. The dependence of the depolarization-induced increase in intraterminal, free Ca2+ on the membrane potential between about -50 to 0 mV was sigmoidal. A maximal increase in cytosolic Ca2+ was reached when the membrane potential was depolarized from the resting level, about -64 mV, to about -40 mV. These results show that in isolated nerve endings the activation of voltage-sensitive Ca2+ channels concomitantly leads to an increase in cytosolic, free Ca2+. Comparison of the results of the present study with the previous electrophysiological observations indicate that Ca2+ channels in synaptosomes, presynaptic nerve terminals of the squid giant synapse and cardiac cells have essentially similar voltage dependency. 相似文献
909.
R E Byrne D Polacek J I Gordon A M Scanu 《The Journal of biological chemistry》1984,259(23):14537-14543
The proteolytic activity directed against apolipoprotein A-II (apo-A-II) which is released from human blood polymorphonuclear cells (PMN) when they are incubated with human plasma high-density lipoprotein-3 (HDL3) was studied to assess the properties and site specificity of the enzyme. When 125I-apo-A-II-labeled HDL3 was incubated with the PMN protease at 37 degrees C, a complete cleavage of apo-A-II was observed which paralleled the formation of bands of approximately 11,000 and 7,000 daltons by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The 7,000-dalton component had the following N-terminal sequence: NH2-Thr-Asp-Tyr-Gly-Lys-Asp-Leu-Met-Glu-Lys. This corresponds to residues 19 through 28 of the intact apo-A-II monomer. Methoxysuccinyl (MeO-Suc)-Ala-Ala-Pro-Val-chloromethylketone-(CH2Cl) caused a 90% inhibition of apo-A-II hydrolysis at the highest concentration tested (6 X 10(-4)M). Besides apo-A-II, the PMN enzyme also hydrolyzed a synthetic substrate, MeO-Suc-Ala-Ala-Pro-Val-4-nitroanilide and its 4-methylcoumaryl-7-amide analogue. The protease appeared to have a mass of 28,000 daltons as assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the [3H]diisopropylfluorophosphate-labeled PMN enzyme. That the PMN enzyme which cleaves apo-A-II is an elastase was derived from the following criteria: 1) cleavage at the Val-X bond in apo-A-II and in the two synthetic substrates studied; 2) prevention of the cleavage by MeO-Suc-Ala-Ala-Pro-Val-CH2Cl, a known specific elastase inhibitor; and 3) a mass comparable to that reported for a pure PMN elastase. These studies establish that apolipoproteins can be suitable substrates for enzymes of the elastase family. 相似文献
910.
A method is described for the subcellular fractionation of goldfish xanthophores. The procedure produces relatively pure fractions of caroteniod droplets, pterinosomes, cytosol and what appears to be plasma membrane. The presence of a distinct pattern of proteins is shown to be associated with the carotenoid droplets. Treatment of the xanthophores with ACTH affects the buoyant density of some carotenoid droplets and stimulates the phosphorylation of a polypeptide associated with the carotenoid droplets. 相似文献