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41.
42.
Serotypic Variation Among Isolates of Ichthyophthirius multifiliis Based on Immobilization 总被引:4,自引:0,他引:4
HARRY W. DICKERSON THEODORE G. CLARK ADAM A. LEFF 《The Journal of eukaryotic microbiology》1993,40(6):816-820
Efforts have been made to determine whether surface antigens could be used as biochemical markers to define strain differences in the parasitic ciliate Ichthyophthirius multifiliis. In previous studies, a wild-type isolate designated G1 was found to have surface proteins analogous to the immobilization antigens of Paramecium and Tetrahymena; rabbit antiserum against this strain immobilizes homologous cells in vitro. It has now been shown for two additional Ichthyophthirius isolates (designated G1.1 and G2) that immobilization antigens are both present and serologically distinct. Proteins of similar size, which cross-react in Western blots with rabbit antisera against immobilization antigens of the G1 strain, are nevertheless found in the G1.1 and G2 isolates. As shown by Southern blotting analysis, the G1.1 and G2 strains also contain genomic DNA sequences which hybridize with an immobilization antigen cDNA from G1 when probed under conditions of reduced stringency. The serotypic differences in immobilization between I. multifiliis isolates appear to be stable over time and provide a means of discriminating strains. In addition to providing a basis for comparative studies, the work described here has implications for the development of vaccines against this important fish parasite. 相似文献
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HARRY K. KENWARD 《Ecological Entomology》1976,1(1):7-17
- 1 Analysis of insect ‘death assemblages’ from ancient natural and anthropogenic deposits can provide valuable information about conditions in the past but the theoretical basis of such investigations is weak, especially with respect to the quantification of habitats on archaeological sites.
- 2 Evidence is presented for the existence of a transported component, termed ‘background fauna’, in death assemblages, as well as the remains of insects derived from the immediate surroundings.
- 3 The background component is derived principally from airborne insects and from bird droppings and may seriously detract from the accuracy of reconstructions of past ecological conditions. Factors affecting its relative importance are discussed.
- 4 As well as including species from distant habitats, the background fauna may be biased in favour of species from temporary habitats.
- 5 Some solutions to the problems presented by the background fauna are discussed. A high proportion of a species or ecological group will usually be evidence that its habitat existed near to the deposit enclosing the fauna, although there may be exceptions.
- 6 The structure of the background rain of insects has probably altered with changes in town and countryside brought about by man. Attempts to recognize background fauna in ancient assemblages must take account of this.
- 7 Many ancient assemblages, especially if small, will give no useful ecological information.
45.
Surface Changes Induced by Immune Serum on Eimeria tenella Sporozoites and Merozoites 总被引:2,自引:0,他引:2
ABSTRACT. The surface of merozoites and sporozoites of Eimeria tenella was affected by incubation with E. tenella -immune chicken serum (ICS). Normal chicken serum (NCS) and heat-inactivated ICS had no effect on the pellicular surface of either developmental stage. Sporozoites formed surface bulges or swellings after 10 min of incubation with ICS, and by 15 min postincubation, the morphology of the sporozoites was distorted by a surface coating of fibrinous material. Merozoites exposed to ICS were similarly coated, but surface swelling was not as severe. The coating formed rapidly and was seen as early as 5 min postincubation. Sporozoites incubated with heat-inactivated ICS supplemented with normal chicken serum were coated with a fibrinous material and in some cases lysed. These data indicated that complement must be present for the surface interaction to occur. 相似文献
46.
HARRY SANABRIA 《American anthropologist》2007,109(3):587-588
Fear of Small Numbers: An Essay on the Geography of Anger . Arjun Appadurai. Durham, NC: Duke University Press, 2006. 153 pp. 相似文献
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48.
OWEN G. HARRY 《The Journal of eukaryotic microbiology》1965,12(2):296-305
SYNOPSIS. The Eugregarine Gregarina garnhami is parasitic in the mid-intestine and caecae of the desert locust Schistocerra gregaria (Forsk). The penetration oi the sporozoite and its development into a three-segmented cephalont is described. At no time in its development is the Fregarine completely intracellular; the so-called intracellular stages of other workers are shown to be the breakdoan products of the intestinal host cells themselves.
After penetrating the host intestinal cell the sporozoite grows rapidly and the anterior intracellular region outgrows the posterior estracellular region. Once the intracellular region reaches a size 13–16 μ in diameter. growth oi this region ceases and growth is now concentrated in the posterior region. When this latter region has reached 20 μ in diameter an annulus appears. This, by growing diametrically across the posterior epimerite region, divides the extracellular region into protomerite and deutomerite.
Detachment of the cephalont is considered to be a passive and not an active process and is dependent on the breakdown and extrusion of host cells. In this process the epimerite may or may not become detached from the protomerite.
A system of lonpitudinal fibres (myonemes) exists within the protomerite, and these play an important role in converting the protomerite into an adhesil-e disc prior to conjugation.
Extensive folding of the epicyte occurs in both the protomerite and deutomerite in the three-segmented cephalont; this folding could not be observed prior to the division of the gregarine into three regions. The epimerite has a dual function in the early stages of development, serving both as an attachment organelle and as an absorptive region across which iood materials may pass from the host cell. 相似文献
After penetrating the host intestinal cell the sporozoite grows rapidly and the anterior intracellular region outgrows the posterior estracellular region. Once the intracellular region reaches a size 13–16 μ in diameter. growth oi this region ceases and growth is now concentrated in the posterior region. When this latter region has reached 20 μ in diameter an annulus appears. This, by growing diametrically across the posterior epimerite region, divides the extracellular region into protomerite and deutomerite.
Detachment of the cephalont is considered to be a passive and not an active process and is dependent on the breakdown and extrusion of host cells. In this process the epimerite may or may not become detached from the protomerite.
A system of lonpitudinal fibres (myonemes) exists within the protomerite, and these play an important role in converting the protomerite into an adhesil-e disc prior to conjugation.
Extensive folding of the epicyte occurs in both the protomerite and deutomerite in the three-segmented cephalont; this folding could not be observed prior to the division of the gregarine into three regions. The epimerite has a dual function in the early stages of development, serving both as an attachment organelle and as an absorptive region across which iood materials may pass from the host cell. 相似文献
49.
Response of African savanna ants to long-term fire regimes 总被引:2,自引:0,他引:2
50.