全文获取类型
收费全文 | 10530篇 |
免费 | 1383篇 |
国内免费 | 3323篇 |
专业分类
15236篇 |
出版年
2024年 | 78篇 |
2023年 | 264篇 |
2022年 | 484篇 |
2021年 | 685篇 |
2020年 | 581篇 |
2019年 | 679篇 |
2018年 | 531篇 |
2017年 | 484篇 |
2016年 | 596篇 |
2015年 | 781篇 |
2014年 | 953篇 |
2013年 | 886篇 |
2012年 | 1091篇 |
2011年 | 959篇 |
2010年 | 694篇 |
2009年 | 604篇 |
2008年 | 643篇 |
2007年 | 575篇 |
2006年 | 594篇 |
2005年 | 486篇 |
2004年 | 411篇 |
2003年 | 343篇 |
2002年 | 336篇 |
2001年 | 221篇 |
2000年 | 198篇 |
1999年 | 152篇 |
1998年 | 78篇 |
1997年 | 67篇 |
1996年 | 51篇 |
1995年 | 53篇 |
1994年 | 45篇 |
1993年 | 41篇 |
1992年 | 63篇 |
1991年 | 61篇 |
1990年 | 59篇 |
1989年 | 47篇 |
1988年 | 33篇 |
1987年 | 41篇 |
1986年 | 35篇 |
1985年 | 38篇 |
1984年 | 31篇 |
1983年 | 25篇 |
1982年 | 20篇 |
1981年 | 11篇 |
1980年 | 16篇 |
1977年 | 9篇 |
1972年 | 12篇 |
1971年 | 9篇 |
1970年 | 9篇 |
1969年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
SEO Dong-Hwan† Seock-Yeon HWANG† Jikhyon HAN Sang-Kyun KOH Iksoo KIM Kang Sun RYU Chi-Young YUN 《Entomological Research》2004,34(3):207-211
This study was performed to explore novel and valuable uses of insect resources, important subjects of the natural compound used in bio‐industries. The whole bodies of two crickets, Gryllus bimaculatus and Teleogryllus emma, selected from medicinal insect species, were carefully ground and treated with 80% EtOH. The insect extracts were solubilized and separated by hexane, butanol, and D.W according to their polarities. Three types of extracts, a D.W fraction (G1) and a boiling extract (G2) of an introduced cricket, G. bimaculatus, and a D.W fraction (T1) of a Korean local cricket, T. emma, were prepared to assay immune stimulating activity of cricket originated compounds. The all of three treated cricket extracts showed to increase IL‐4, IFN‐, and TNF‐α. Among those extract, extract G2, boiled extract from G. bimaculatus, was the best immune–enhancing fraction. The results of this study could be fundamental information for further works to use insects as natural resources having plenty of potentials and varieties. 相似文献
72.
小麦籽粒充实期氮素的吸收和再分配及6-苄氨基嘌呤的调节作用 总被引:3,自引:0,他引:3
小麦从开花至成熟,其根、茎和叶中的N素不断地进行着分配和再分配,主要是向穗子输出。成熟时穗N含量占植株总N的82%。在总N净输出的同时各营养器官也有相当程度的对当前土壤N的净吸收。在籽粒充实阶段用BA处理叶式穗均影响营养器官及穗子的N素吸收、分配和再分配。 相似文献
73.
Processing of global and local properties——An analysis with event-related brain potentials 总被引:3,自引:1,他引:2
The different processing of global and local properties of compound visual stimuli was studied with event-related brain potentials (ERPs) in the present experiment. It was found that, compared with the identification of global properties, the discrimination of local properties elicited longer RTs, lower accuracies, increased amplitudes of P1, decreased amplitudes of N1, and longer latencies of N2 and P3. The conflict of global and local properties increased the amplitudes of P2, decreased the amplitudes of P3, and prolonged latencies of N2 and P3. These results indicated that the advantage of global processing occurs at an early perceptual stage, and the attentional mechanisms for global and local processing may be different. 相似文献
74.
在主动脉与肾动脉缩窄造成的慢性心功能不全大鼠,血浆儿茶酚胺浓度增高;心脏β-肾上腺素受体(β-AR)数量增加,其中β_1-AR及其mRNA增加,而β_2-AR及其mRNA不变;左心房异丙基肾上腺素(ISO)浓度-收缩效应曲线右移;而心肌ISO浓度-cAMP蓄积曲线无显著改变;血淋巴细胞β-AR数量显著减少.结果提示心功能不全时心脏β_1-AR数量增多,但其介导的正性变力效应反而降低,在cAMP生成以后的信号转导过程或心肌收缩成分功能存在障碍,而血淋巴细胞β-AR的改变与心脏β-AR的功能改变平行. 相似文献
75.
Aberrant Gag protein composition of a human immunodeficiency virus type 1 vif mutant produced in primary lymphocytes. 总被引:7,自引:6,他引:1
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Productive, spreading infection of peripheral blood lymphocytes (PBL) with human immunodeficiency virus type 1 (HIV-1) requires the viral protein Vif. To study the requirement for vif in this system, we infected PBL with a phenotypically complemented HIV-1 clone mutated in vif. Progeny virus was produced which was noninfectious in PBL but replicated in SupT1 cells. Analysis of metabolically labeled proteins of sedimentable extracellular particles made in PBL by radioimmunoprecipitation with either serum from a patient with AIDS or a monoclonal antibody reactive with HIV-1 Gag proteins revealed that vif-negative but not wild-type particles carry higher levels of p55, p41, and p38 Gag-specific proteins compared with those of p24. Similar results were obtained with sucrose-purified virions. Our data indicate that vif plays a role in Gag protein processing or in incorporation of processed Gag products into mature virions. The presence of unprocessed precursor Gag polyprotein (Pr55gag) and other Gag processing intermediates in PBL-derived vif-negative extracellular particles may contribute to the reduced infectivity of this virus. 相似文献
76.
Ras transformation results in an elevated level of cyclin D1 and acceleration of G1 progression in NIH 3T3 cells. 总被引:14,自引:7,他引:7
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
J J Liu J R Chao M C Jiang S Y Ng J J Yen H F Yang-Yen 《Molecular and cellular biology》1995,15(7):3654-3663
Ectopic overexpression of v-H-Ras protein in NIH 3T3 cells resulted in cellular transformation and an acceleration of G1 progression of these cells. A shortened G1 phase was found to be associated with an increased level of cyclin D1 but not cyclin E protein. Using an antisense blocking method, reduced synthesis of cyclin D1 in v-H-Ras transformants resulted in a slower G1 progression rate of these cells. Although constitutive overexpression of cyclin D1 in NIH 3T3 cells accelerated G1 progression, cells remained untransformed. Furthermore, inhibition of cyclin D1 synthesis greatly impaired the soft-agar cloning efficiency of v-H-Ras transformants. These results suggest that increased expression of cyclin D1 is necessary but not sufficient for the transforming activity of v-H-Ras. Similar effect on cell cycle progression was also observed in Raf-transformed cells. In addition to cyclin D1, cyclin E protein was found to be elevated in Src transformants. This may account for the further shortening of the G1 phase of these cells. Activation of an additional Ras-independent pathway was suggested to be responsible for the further acceleration of the G1 phase in Src transformants. 相似文献
77.
中枢ACTH受体研究进展 总被引:2,自引:0,他引:2
除垂体以外,中枢神经系统也含有促肾上腺皮质激素(ACTH)能神经元,其神经纤维在中枢具有较广泛的投射。ACTH相关肽类在中枢发挥着多种生理功能。近年来对于中枢ACTH受体的研究取得很大的进展,现已确认ACTH结合位点在中枢具有广泛的分布。新近克隆出的四种ACTH受体中,有两种是中枢神经系统占优势的受体亚型。 相似文献
78.
79.
80.
Kallikrein activation of a high molecular weight atrial peptide 总被引:5,自引:0,他引:5
M G Currie D M Geller J Chao H S Margolius P Needleman 《Biochemical and biophysical research communications》1984,120(2):461-466
Mammalian atrial extracts contain bioactive peptides that exert profound effects upon renal function and isolated smooth muscle preparations. Gel filtration chromatography of rat atrial extract separates the activity into two peaks having apparent molecular weights of 20,000 to 30,000 and less than 10,000. Mild proteolytic treatment (trypsin 1 U/ml) of the high molecular weight fraction enhances the smooth muscle relaxant activity of this fraction and concomitantly reduces the apparent molecular weight of this fraction to less than 10,000. In this report we show that urinary and submaxillary kallikrein enhances the activity of rat atrial extracts in a similar fashion. Pretreatment of the high molecular weight fraction with either kallikrein (1 microgram/ml) enhances the smooth muscle relaxant activity of this fraction. Similar treatment of the low molecular weight fraction had no effect. The enhancement of the bioactivity of the high molecular weight substance(s) by the kallikreins was abolished by aprotinin but was unaffected by soybean trypsin inhibitor. These results suggest that exogenous addition of tissue kallikrein activates a high molecular weight peptide by limited proteolysis. Analysis of the kallikrein-treated high molecular weight peptide fraction by gel filtration indicates that the biological activity comigrates with the low molecular weight peptides present in the original atrial extract. 相似文献