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Development of a dot immunobinding assay to detect Phytophthora spp. in naturally dark-rooted woody plants 总被引:1,自引:0,他引:1
An indirect dot immunobinding assay (DIBA) with a PAb raised against an isolate of P. cinnumomi was evaluated to detect Phytophthora spp. in naturally dark-rooted woody plants. Screening of different modifications of the procedure were done with root samples of Chamaecyparis lawsoniana non-infected and infected with P. cinnamomi. With the optimised DIBA procedure, root infection could be diagnosed by a clearly defined halo around the spot and by a colour change in the spot using Fast Red or Fast Blue substrate. Detection of different Phytophthora species in Chamaecyparis plants from commercial nurseries was successful with the optimised procedure. 相似文献
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ABSTRACT Three vocalisation types of the másafuera rayadito Aphrastura masafuerae are described using sonograms: common call, alarm call, and song. They are analysed according to their structure, kHz-frequency, duration and time of day. The common call can be uttered singly or in a series with regular pauses. This intensive call is heard even through fog and wind and seems to be used for contacting the partner as well as signalling the home range to neighbours. The alarm call is a continuous series of similar syllables without pauses. The song is given exclusively in the morning before sunrise by only one individual of the pair. It has one type of phrase only, which is repeated. Phrases are interrupted by regular pauses. These vocalisation types are clearly different from each other, but also have features in common: the monotonous structure, repetition of units and temporal frequency of elements. 相似文献
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