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931.
932.
DNA-binding agents have been considered as an established opportunity for the development of anticancer drugs and DNA fluorescence probes. This work reported the synthesis of two novel carbazole derivatives (1 and 2) and investigated their DNA binding properties, living cell image, and cytotoxicity. The results demonstrated that both compounds presented the higher binding affinity to G-quadruplex than to duplex DNA by means of UV–Vis absorption titration and fluorescent intercalator displacement. Continuous variation analysis indicated that their binding stoichiometries of the compound/G-quadruplex were near 2 except the compound/bcl-2. Circular dichroism spectra showed that both compounds had no influence on the conformation of G-quadruplexes. Fluorescence titrations indicated that 2 had the potential to be a G-quadruplex selective fluorescent probe, while 1 could be used as a fluorescent probe for duplex DNA. Confocal fluorescence images indicated that both compounds could enter the living HepG2 cells, and 1 mainly located in nucleus whereas 2 mainly distributed in cytoplasm. DNase and RNase digest tests indicated that both compounds could enter into the nucleus and interact with duplex DNA, especially, 2 could interact with RNA and/or G-quadruplex DNA. They also exhibited an obvious antiproliferative activity to HepG2 by using MTT assays, with IC50 values of 2.7 and 9.5?μM for 1 and 2, respectively. 相似文献
933.
Alberto V. Borges Gaëlle Speeckaert Willy Champenois Mary I. Scranton Nathalie Gypens 《Ecosystems》2018,21(4):583-599
Dissolved CH4 concentrations in the Belgian coastal zone (North Sea) ranged between 670 nmol l?1 nearshore and 4 nmol l?1 offshore. Spatial variations of CH4 were related to sediment organic matter (OM) content and gassy sediments. In nearshore stations with fine sand or muddy sediments, the CH4 seasonal cycle followed water temperature, suggesting methanogenesis control by temperature in these OM-rich sediments. In offshore stations with permeable sediments, the CH4 seasonal cycle showed a yearly peak following the chlorophyll-a spring peak, suggesting that in these OM-poor sediments, methanogenesis depended on freshly produced OM delivery. This does not exclude the possibility that some CH4 might originate from dimethylsulfide (DMS) or dimethylsulfoniopropionate (DMSP) or methylphosphonate transformations in the most offshore stations. Yet, the average seasonal CH4 cycle was unrelated to those of DMS(P), very abundant during the Phaeocystis bloom. The annual average CH4 emission was 126 mmol m?2 y?1 in the most nearshore stations (~4 km from the coast) and 28 mmol m?2 y?1 in the most offshore stations (~23 km from the coast), 1260–280 times higher than the open ocean average value (0.1 mmol m?2 y?1). The strong control of CH4 by sediment OM content and by temperature suggests that marine coastal CH4 emissions, in particular in shallow areas, should respond to future eutrophication and warming of climate. This is supported by the comparison of CH4 concentrations at five stations obtained in March 1990 and 2016, showing a decreasing trend consistent with alleviation of eutrophication in the area. 相似文献
934.
Unlike any great apes, humans have expanded into a wide variety of habitats during the course of evolution, beginning with the transition by australopithecines from forest to savanna habitation. Novel environments are likely to have imposed hominids a demographic challenge due to such factors as higher predation risk and scarcer food resources. In fact, recent studies have found a paucity of older relative to younger adults in hominid fossil remains, indicating considerably high adult mortality in australopithecines, early Homo, and Neanderthals. It is not clear to date why only human ancestors among all hominoid species could survive in these harsh environments. In this paper, we explore the possibility that hominids had shorter interbirth intervals to enhance fertility than the extant apes. To infer interbirth intervals in fossil hominids, we introduce the notion of the critical interbirth interval, or the threshold length of birth spacing above which a population is expected to go to extinction. We develop a new method to obtain the critical interbirth intervals of hominids based on the observed ratios of older adults to all adults in fossil samples. Our analysis suggests that the critical interbirth intervals of australopithecines, early Homo, and Neanderthals are significantly shorter than the observed interbirth intervals of extant great apes. We also discuss possible factors that may have caused the evolutionary divergence of hominid life history traits from those of great apes. 相似文献
935.
936.
Both global change and biological invasions threaten biodiversity worldwide. However, their interactions and related mechanisms are still not well elucidated. To elucidate potential traits contributing to invasiveness and whether ongoing increase in CO2 aggravates invasions, noxious invasive Wedelia trilobata and native Wedelia urticifolia and Wedelia chinensis were compared under ambient and doubled atmospheric CO2 concentrations in terms of growth, biomass allocation, morphology, and physiology. The invader had consistently higher leaf mass fraction (LMF) and specific leaf area than the natives, contributing to a higher leaf area ratio, and therefore to faster growth and invasiveness. The higher LMF of the invader was due to lower root mass fraction and higher fine root percent. On the other hand, the invader allocated a higher fraction of leaf nitrogen (N) to photosynthetic apparatus, which was associated with its higher photosynthetic rate, and resource use efficiency. All these traits collectively contributed to its invasiveness. CO2 enrichment increased growth of all studied species by increasing actual photosynthesis, although it decreased photosynthetic capacities due to decreased leaf and photosynthetic N contents. Responses of the invasive and native plants to elevated CO2 were not significantly different, indicating that the ongoing increase in CO2 may not aggravate biological invasions, inconsistent with the prevailing results in references. Therefore, more comparative studies of related invasive and native plants are needed to elucidate whether CO2 enrichment facilitates invasions. 相似文献
937.
Port landing specimens of torpedo scad, Megalaspis cordyla were collected at monthly intervals from August 2013 to June 2014 from the northern Arabian Sea coast of Pakistan. The life history characteristics revealed that torpedo scad has a prolonged breeding season which occurred with two peaks: from May to June and from November to February. The spawning seasonality was also validated with microscopic gonad examination.The highest GSI value was noted in May and January whereas the lowest in November was recorded for both sexes. The age at which individuals of both sexes become 50% mature (t50) was estimated as 2.44 year. Life span (tmax) calculated as 3.96 years for male and females. The size of 270 and 265 mm at which 50% individuals attained sexual maturity (L50) was determined for males and females respectively. The reproductive potential (fecundity)was made from ten ripe females ranged from 29–43.8 cm TL and weighing 220–540 g of IV–V developmental stage was enumerated as 32306–236677 eggs (CV = 2.135) with an average number of eggs 86544 counted per ripe female/season. This study contains fundamental data of an important fish species in the northern Arabian Sea coast of Pakistan. It provides vital information to the fisheries managers for implementation on sustainable use of the resource during spawning season. 相似文献
938.
Dicken CL Israel DD Davis JB Sun Y Shu J Hardin J Neal-Perry G 《Biology of reproduction》2012,87(2):51
The mechanism(s) by which vitamin D(3) regulates female reproduction is minimally understood. We tested the hypothesis that peripubertal vitamin D(3) deficiency disrupts hypothalamic-pituitary-ovarian physiology. To test this hypothesis, we used wild-type mice and Cyp27b1 (the rate-limiting enzyme in the synthesis of 1,25-dihydroxyvitamin D(3)) null mice to study the effect of vitamin D(3) deficiency on puberty and reproductive physiology. At the time of weaning, mice were randomized to a vitamin D(3)-replete or -deficient diet supplemented with calcium. We assessed the age of vaginal opening and first estrus (puberty markers), gonadotropin levels, ovarian histology, ovarian responsiveness to exogenous gonadotropins, and estrous cyclicity. Peripubertal vitamin D(3) deficiency significantly delayed vaginal opening without affecting the number of GnRH-immunopositive neurons or estradiol-negative feedback on gonadotropin levels during diestrus. Young adult females maintained on a vitamin D(3)-deficient diet after puberty had arrested follicular development and prolonged estrous cycles characterized by extended periods of diestrus. Ovaries of vitamin D(3)-deficient Cyp27b1 null mice responded to exogenous gonadotropins and deposited significantly more oocytes into the oviducts than mice maintained on a vitamin D(3)-replete diet. Estrous cycles were restored when vitamin D(3)-deficient Cyp27b1 null young adult females were transferred to a vitamin D(3)-replete diet. This study is the first to demonstrate that peripubertal vitamin D(3) sufficiency is important for an appropriately timed pubertal transition and maintenance of normal female reproductive physiology. These data suggest vitamin D(3) is a key regulator of neuroendocrine and ovarian physiology. 相似文献
939.
Alfredo Saldaña Christopher H. Lusk Wilfredo L. Gonzáles Ernesto Gianoli 《Evolutionary ecology》2007,21(5):651-662
Unlike other species of the genus Blechnum, the fern Blechnum chilense occurs in a wide range of habitats in Chilean temperate rainforest, from shaded forest understories to abandoned clearings
and large gaps. We asked if contrasting light environments can exert differential selection on ecophysiological traits of
B. chilense. We measured phenotypic selection on functional traits related to carbon gain: photosynthetic capacity (A
max), dark respiration rate (R
d), water use efficiency (WUE), leaf size and leaf thickness in populations growing in gaps and understorey environments. We
assessed survival until reproductive stage and fecundity (sporangia production) as fitness components. In order to determine
the potential evolutionary response of traits under selection, we estimated the genetic variation of these traits from clonally
propagated individuals in common garden experiments. In gaps, survival of B. chilense was positively correlated with WUE and negatively correlated with leaf size. In contrast, survival in shaded understories
was positively correlated with leaf size. We found positive directional fecundity selection on WUE in gaps population. In
understories, ferns of lower R
d and greater leaf size showed greater fecundity. Thus, whereas control of water loss was optimized in gaps, light capture
and net carbon balance were optimized in shaded understories. We found a significant genetic component of variation in WUE,
R
d and leaf size. This study shows the potential for evolutionary responses to heterogeneous light environments in functional
traits of B. chilense, a unique fern species able to occupy a broad successional niche in Chilean temperate rainforest. 相似文献
940.
Stephan Herget Philip V Toukach René Ranzinger William E Hull Yuriy A Knirel Claus-Wilhelm von der Lieth 《BMC structural biology》2008,8(1):35