全文获取类型
收费全文 | 500篇 |
免费 | 30篇 |
国内免费 | 1篇 |
出版年
2023年 | 9篇 |
2022年 | 10篇 |
2021年 | 24篇 |
2020年 | 9篇 |
2019年 | 13篇 |
2018年 | 21篇 |
2017年 | 18篇 |
2016年 | 23篇 |
2015年 | 31篇 |
2014年 | 26篇 |
2013年 | 52篇 |
2012年 | 51篇 |
2011年 | 37篇 |
2010年 | 21篇 |
2009年 | 20篇 |
2008年 | 30篇 |
2007年 | 18篇 |
2006年 | 20篇 |
2005年 | 22篇 |
2004年 | 16篇 |
2003年 | 7篇 |
2002年 | 6篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1986年 | 5篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1966年 | 1篇 |
1958年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有531条查询结果,搜索用时 15 毫秒
41.
In this work, we report the thermodynamic and ultrasonic properties of ascorbic acid (vitamin C) in water and in presence of newly synthesized ammonium based protic ionic liquid (diethylethanolammonium propionate) as a function of concentration and temperature. Apparent molar volume and apparent molar isentropic compression, which characterize the solvation state of ascorbic acid (AA) in presence of protic ionic liquid (PIL) has been determined from precise density and speed of sound measurements at temperatures (293.15 to 328.15) K with 5 K interval. The strength of molecular interactions prevailing in ternary solutions has been discussed on the basis of infinite dilution partial molar volume and partial molar isentropic compression, corresponding volume of transfer and interaction coefficients. Result has been discussed in terms of solute-solute and solute-solvent interactions occurring between ascorbic acid and PIL in ternary solutions (AA + water + PIL). 相似文献
42.
Ramansu Goswami Sudipto Mandal Sandip Mandal Pratap Kumar Padhy Santanu Ray Shibnath Mazumder 《Biologia》2014,69(7):825-833
Aeromonas hydrophila is frequently reported from arsenic affected areas. Present study was aimed to determine the effect of arsenic and temperature on growth of A. hydrophila. The bacteria were isolated from naturally infected fish from a water body in Birbhum, West-Bengal, India, which is reported to be an arsenic-free area. Arsenic concentration in natural aquatic reservoirs (e.g., pond, lake or river) varies from 0–6 mg/L. No significant change in bacterial growth was observed within this range of arsenic exposure. However, variation in temperature impacted the growth of A. hydrophila. A single dimension model was constructed using simple logistic equation. Rate parameters of the model were derived from the experimental observations. Comparison of model results and laboratory observations gives a good conformity regarding the effect of variation of arsenic concentration and temperature change on growth of this bacterium. From the analysis of this model we further get the idea that the maximum growth of A. hydrophila is supposed to be at 31.4°C in absence of arsenic, whereas at 477 mg/L arsenic concentration, the growth of the bacteria totally stops at 30°C. 相似文献
43.
Sheelendra Pratap Singh Dinesh K. Yadav Preeti Rawat Rakesh Maurya Girish Kumar Jain 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2010,878(3-4):391-397
A new simple, rapid, sensitive and accurate quantitative detection method using liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) for the measurement of formononetin (FMN) and daidzein (DZN) levels in rat plasma is described. Analytes were separated on a Supelco Discovery C18 (4.6 × 50 mm, 5.0 μm) column with acetonitrile: methanol (50:50, v/v) and 0.1% acetic acid in the ratio of 90:10 (v/v) as a mobile phase. The method was proved to be accurate and precise at linearity range of 5–100 ng/mL with a correlation coefficient (r) of ≥0.996. The intra- and inter-day assay precision ranged from 1.66–6.82% and 1.87–6.75%, respectively; and intra- and inter-day assay accuracy was between 89.98–107.56% and 90.54–105.63%, respectively for both the analytes. The lowest quantitation limit for FMN and DZN was 5.0 ng/mL in 0.1 mL of rat plasma. Practical utility of this new LC–MS/MS method was demonstrated in a pharmacokinetic study in rats following intravenous administration of FMN. 相似文献
44.
A bioinformatic filter for improved base-call accuracy and polymorphism detection using the Affymetrix GeneChip® whole-genome resequencing platform 下载免费PDF全文
Gagan A. Pandya Michael H. Holmes Sirisha Sunkara Andrew Sparks Yun Bai Kathleen Verratti Kelly Saeed Pratap Venepally Behnam Jarrahi Robert D. Fleischmann Scott N. Peterson 《Nucleic acids research》2007,35(21):e148
DNA resequencing arrays enable rapid acquisition of high-quality sequence data. This technology represents a promising platform for rapid high-resolution genotyping of microorganisms. Traditional array-based resequencing methods have relied on the use of specific PCR-amplified fragments from the query samples as hybridization targets. While this specificity in the target DNA population reduces the potential for artifacts caused by cross-hybridization, the subsampling of the query genome limits the sequence coverage that can be obtained and therefore reduces the technique's resolution as a genotyping method. We have developed and validated an Affymetrix Inc. GeneChip® array-based, whole-genome resequencing platform for Francisella tularensis, the causative agent of tularemia. A set of bioinformatic filters that targeted systematic base-calling errors caused by cross-hybridization between the whole-genome sample and the array probes and by deletions in the sample DNA relative to the chip reference sequence were developed. Our approach eliminated 91% of the false-positive single-nucleotide polymorphism calls identified in the SCHU S4 query sample, at the cost of 10.7% of the true positives, yielding a total base-calling accuracy of 99.992%. 相似文献
45.
Mishra Vipul Singh Pooja Kushwaha Bishwajit Kumar Tripathi Durgesh Kumar Corpas Francisco J. Singh Vijay Pratap 《Plant Growth Regulation》2022,96(1):1-23
Plant Growth Regulation - Ion transporters are essential for plant growth and development, and play key roles not only in acquisition/ transportation of essential ions from the surrounding and... 相似文献
46.
Fatima Abreeq Husain Tajammul Suhel Mohammad Prasad Sheo Mohan Singh Vijay Pratap 《Journal of Plant Growth Regulation》2022,41(1):163-177
Journal of Plant Growth Regulation - It is a well-established fact that nitric oxide (NO) is a multifaceted signaling molecule, which plays diverse role in organisms. In the past two decades,... 相似文献
47.
48.
Giang Ngan Khong Pratap Kumar Pati Frédérique Richaud Boris Parizot Przemyslaw Bidzinski Chung Duc Mai Martine Bès Isabelle Bourrié Donaldo Meynard Tom Beeckman Michael Gomez Selvaraj Ishitani Manabu Anna-Maria Genga Christophe Brugidou Vinh Nang Do Emmanuel Guiderdoni Jean-Benoit Morel Pascal Gantet 《Plant physiology》2015,169(4):2935-2949
49.
50.
Arnab Nayek Parth Sarthi Sen Gupta Shyamashree Banerjee Vishma Pratap Sur Pratyay Seth Sunit Das Rifat Nawaz Ul Islam Amal Kumar Bandyopadhyay 《Bioinformation》2015,11(8):413-415
AvailabilityADSBET2 is freely available at http://sourceforge.net/projects/ADSBET2/ for all users. 相似文献