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The presence of small amounts of 3β-hydroxy-Δ5-C19- and C21-steroid oxidoreductase in the microsomal fraction of rat liver is shown. NAD was the preferred cofactor. Km for the oxidation of dehydroepiandrosterone (3β-hydroxy-5-androsten-17-one) into androstenedione (4-androstene-3,17-dione) was 3 × 10−6 M. In similarity to the adrenal and gonadal 3β-hydroxy-Δ5-C19-steroid oxidoreductase, but in contrast to the hepatic 3β-hydroxy-Δ5-C27-steroid oxidoreductase involved in the biosynthesis of bile acids, the hepatic 3β-hydroxy-Δ5-C19-steroid oxidoreductase was inhibited by the 3β-hydroxy-Δ5-steroid oxidoreductase inhibitor, 2α-cyano-4,4,17-trimethyl-17β-hydroxy-5-androsten-3-one, and the activity was greatly reduced with microsomes from immature rats. 相似文献
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Identification and functional characterization of a novel mitochondrial thioredoxin system in Saccharomyces cerevisiae 总被引:2,自引:0,他引:2
Pedrajas JR Kosmidou E Miranda-Vizuete A Gustafsson JA Wright AP Spyrou G 《The Journal of biological chemistry》1999,274(10):6366-6373
The so-called thioredoxin system, thioredoxin (Trx), thioredoxin reductase (Trr), and NADPH, acts as a disulfide reductase system and can protect cells against oxidative stress. In Saccharomyces cerevisiae, two thioredoxins (Trx1 and Trx2) and one thioredoxin reductase (Trr1) have been characterized, all of them located in the cytoplasm. We have identified and characterized a novel thioredoxin system in S. cerevisiae. The TRX3 gene codes for a 14-kDa protein containing the characteristic thioredoxin active site (WCGPC). The TRR2 gene codes for a protein of 37 kDa with the active-site motif (CAVC) present in prokaryotic thioredoxin reductases and binding sites for NADPH and FAD. We cloned and expressed both proteins in Escherichia coli, and the recombinant Trx3 and Trr2 proteins were active in the insulin reduction assay. Trx3 and Trr2 proteins have N-terminal domain extensions with characteristics of signals for import into mitochondria. By immunoblotting analysis of Saccharomyces subcellular fractions, we provide evidence that these proteins are located in mitochondria. We have also constructed S. cerevisiae strains null in Trx3 and Trr2 proteins and tested them for sensitivity to hydrogen peroxide. The Deltatrr2 mutant was more sensitive to H2O2, whereas the Deltatrx3 mutant was as sensitive as the wild type. These results suggest an important role of the mitochondrial thioredoxin reductase in protection against oxidative stress in S. cerevisiae. 相似文献
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Matthews J Wihlén B Heldring N MacPherson L Helguero L Treuter E Haldosén LA Gustafsson JA 《The Biochemical journal》2007,406(2):343-353
In the present study we examined the ability of 3,3',4,4',5-pentachlorinated biphenyl [PCB126 (polychlorinated biphenyl 126)], a prototypical AHR (aryl hydrocarbon receptor) agonist, and 2,2',4,6,6'-PCB (PCB104), which does not activate AHR, to induce the recruitment of ERalpha (oestrogen receptor alpha) to CYP1A1 (cytochrome P4501A1 gene) and CYP1B1 promoters in T-47D human breast cancer cells and other cell lines. PCB126 treatment strongly induced CYP1A1 and CYP1B1 mRNA expression that was unaffected by co-treatment with E2 (17beta-oestradiol). PCB104 failed to induce changes in either CYP1A1 or CYP1B1 expression levels. ChIP (chromatin immunoprecipitation) assays show that PCB126, but not PCB104, increased the promoter occupancy by ERalpha to CYP1A1 and CYP1B1 promoters. Co-treatment with PCB126+E2 significantly enhanced the promoter occupancy of ERalpha at CYP1A1, whereas co-treatment with PCB126+4-hydroxytamoxifen or ICI182,780 did not. Competitive binding studies revealed that neither PCB126 nor PCB104 bound to ERalpha. HEK-293 cells (human embryonic kidney-293 cells) stably transfected with ERalpha showed significantly higher PCB126-induced CYP1A1 expression compared with empty vector controls, whereas no increase was observed in cells stably transfected with ERalpha lacking its N-terminal AF1 (activation function-1) domain (ERalphaDeltaAF1). Despite no increase in AHR-mediated gene expression, ChIP assays revealed that ERalphaDeltaAF1 was present at CYP1A1 and CYP1B1 promoters. HC11 mouse mammary cells stably expressing shRNA (small-hairpin RNA) against ERalpha showed an 8-fold reduction in PCB126-dependent Cyp1a1 expression. Our results provide further evidence that AHR agonists induce ERalpha promoter occupancy at AHR target genes through indirect activation of ERalpha, and support a role for ERalpha in AHR transactivation. 相似文献