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91.
Volokhina I Gusev Y Mazilov S Chumakov M 《Journal of bioinformatics and computational biology》2012,10(2):1241009
The transfer of single-stranded (ss) T-DNA from soil bacteria of the genus Agrobacterium with the help of the VirE2 protein, which possibly mediates the delivery of ss-T-DNA across the cell membrane, was demonstrated earlier, but how VirE2 participates in ssDNA transfer across artificial and natural membranes is not known. Using computational methods, we reconstructed model structures composed of two and four VirE2 proteins and showed by the MOLE program the formation of pores with channel diameters of 1.2-1.6 and 1.4-4.6 nm in a model structure formed from two and four VirE2 molecules, respectively. Using light scattering, we recorded the size distribution for recombinant VirE2-dependent complexes in aqueous solutions and found that VirE2 in a buffer solution is present as a complex made up of two or more proteins. We revealed single, long-lived jumps in voltage-dependent membrane conductance during coincubation of planar black membranes with the VirE2 protein. On the addition of VirE2 and FAM-labeled oligonucleotides to HeLa cells, the fluorescence intensity for the cells increased by 56% as compared to that for cells incubated only with oligonucleotides. 相似文献
92.
Leonov AV Gusev VV Gerasimov VN 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2006,(7):94-97
Results of study of capsule formation and immunologic properties of capsular antigen of attenuated strain LA-25 of Pasteurella multocida grown on liquid medium from non-food materials and Hottinger's broth depending on cultivation time are presented. 相似文献
93.
Bauer S Gusev BI Pivina LM Apsalikov KN Grosche B 《Radiatsionnaia biologiia, radioecologiia / Rossi?skaia akademiia nauk》2006,46(5):611-618
This paper describes the Semipalatinsk historical cohort study and, in particular, examines the association between combined external and internal radiation exposure and esophagus cancer. Esophagus cancer is the most frequent single cancer site in the cause of death follow-up for the Semipalatinsk cohort. Set up in the 1960s, this historical cohort included 10 exposed settlements in the vicinity of the Semipalatinsk nuclear test site in East Kazakhstan as well as 6 comparison settlements in a low exposure area of the same region. The external and internal radiation doses to the population of the settlements under study were mainly due to local fallout from atmospheric nuclear testing (1949-1962). The database includes dosimetry and health information for 19.545 inhabitants of exposed and comparison villages in the Semipalatinsk region, comprising a total of 582.750 person-years of follow-up between 1960 and 1999. Cumulative effective dose estimates in this cohort range from 20 mSv to -4 Sv, with a mean dose of 634 mSv in the exposed group. Relative risks were calculated in terms of rate ratios, using a Poisson regression model for grouped person-time data. Esophagus cancer was found substantially elevated, with a statistically significant increase of the relative risk with dose and an ERR/Sv of 2.37 (1.45; 3.28) for the total cohort. If the data set was restricted to the exposed group only, the ERR/Sv was found considerably lower (0.18 (-0.16; 0.52)), whereas the dose-response remained significant only in women. Overall, our results based on the Semipalatinsk historical cohort indicate an association between fallout exposure and the risk of esophagus cancer that should be further investigated. 相似文献
94.
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96.
The recently published review by Dreiza et al. (Cell Stress and Chaperones DOI ) dealing with the functional role of HSPB6 in muscle regulation is critically analyzed. Published data indicate that the
chaperone-like activity of HSPB6 is comparable with that of HSPB5 and that phosphorylation of HSPB6 does not affect its oligomeric
structure. Different hypotheses concerning the molecular mechanisms of HSPB6 action on smooth muscle contraction and on the
reorganization of the cytoskeleton are compared, and it is concluded that although HSPB6 is not a genuine actin-binding protein,
it can affect the actin cytoskeleton indirectly. Phosphorylated HSPB6 interacts with 14-3-3 and thereby displaces other binding
partners of 14-3-3; among them, certain phosphatases, protein kinases, and various actin-binding proteins, which can participate
in the reorganization of the actin cytoskeleton. In addition, HSPB6 seems to regulate the activity of certain protein kinases.
All of these processes are dependent on HSPB6 phosphorylation which in turn might be regulated by the formation of heterooligomeric
complexes of HSPB6 with other small heat shock proteins. 相似文献
97.
Richard Cornette Yasushi Kanamori Masahiko Watanabe Yuichi Nakahara Oleg Gusev Kanako Mitsumasu Keiko Kadono-Okuda Michihiko Shimomura Kazuei Mita Takahiro Kikawada Takashi Okuda 《The Journal of biological chemistry》2010,285(46):35889-35899
Some organisms are able to survive the loss of almost all their body water content, entering a latent state known as anhydrobiosis. The sleeping chironomid (Polypedilum vanderplanki) lives in the semi-arid regions of Africa, and its larvae can survive desiccation in an anhydrobiotic form during the dry season. To unveil the molecular mechanisms of this resistance to desiccation, an anhydrobiosis-related Expressed Sequence Tag (EST) database was obtained from the sequences of three cDNA libraries constructed from P. vanderplanki larvae after 0, 12, and 36 h of desiccation. The database contained 15,056 ESTs distributed into 4,807 UniGene clusters. ESTs were classified according to gene ontology categories, and putative expression patterns were deduced for all clusters on the basis of the number of clones in each library; expression patterns were confirmed by real-time PCR for selected genes. Among up-regulated genes, antioxidants, late embryogenesis abundant (LEA) proteins, and heat shock proteins (Hsps) were identified as important groups for anhydrobiosis. Genes related to trehalose metabolism and various transporters were also strongly induced by desiccation. Those results suggest that the oxidative stress response plays a central role in successful anhydrobiosis. Similarly, protein denaturation and aggregation may be prevented by marked up-regulation of Hsps and the anhydrobiosis-specific LEA proteins. A third major feature is the predicted increase in trehalose synthesis and in the expression of various transporter proteins allowing the distribution of trehalose and other solutes to all tissues. 相似文献
98.
Yashin A. Yu. Bulanin V. V. Petrov A. V. Gusev V. K. Kurskiev G. S. Minaev V. B. Patrov M. I. Petrov Yu. V. 《Plasma Physics Reports》2020,46(7):683-688
Plasma Physics Reports - Here we report the results of the turbulence study in the high-confinement mode (H-mode) with and without edge localized modes (ELMs). The study was performed by the... 相似文献
99.
Structure and properties of small heat shock proteins (sHsp) and their interaction with cytoskeleton proteins 总被引:6,自引:0,他引:6
The modern classification of small heat shock proteins (sHsp) is presented and peculiarities of their primary structure and the mechanism of formation of oligomeric complexes are described. Data on phosphorylation of sHsp by different protein kinases are presented and the effect of phosphorylation on oligomeric state and chaperone activity of sHsp is discussed. Intracellular location of sHsp under normal and stress conditions is described and it is emphasized that under certain condition sHsp interact with different elements of cytoskeleton. The literature concerning the effect of sHsp on polymerization of actin in vitro is analyzed. An attempt is made to compare effects of sHsp on polymerization of actin in vitro with the results obtained on living cells under normal conditions and after heat shock or hormone action. The literature concerning possible effects of sHsp on cell motility is also analyzed. 相似文献
100.
Exploring Cryptic Diversity and Distribution Patterns in the Mallomonas kalinae/rasilis Species Complex with a Description of a New Taxon—Mallomonas furtiva sp. nov. 下载免费PDF全文
Evgeniy S. Gusev Dora Čertnerová Magda Škaloudová Pavel Škaloud 《The Journal of eukaryotic microbiology》2018,65(1):38-47
A complex of closely related Mallomonas taxa belonging to the section Papillosae, M. kalinae ?ezá?ová and M. rasilis Dürrschmidt, has been studied in detail by molecular and morphometric methods. Our investigations uncovered the existence of a new species found in water bodies in Vietnam, which we describe here as Mallomonas furtiva sp. nov. This taxon is morphologically very similar to M. kalinae , from which it differs by minute, but statistically significant morphological differences on the structure of silica scales. Indeed, the principal component analysis of morphological traits measured on silica scales significantly separates all three species in the complex. Mallomonas kalinae and M . furtiva differ by number of papillae on the shield and the dome, as well as by the scale sizes. Likewise, Mallomonas rasilis and M. furtiva are primarily differentiated by the absence of submarginal anterior ribs on silica scales of the former species. Phylogenetic analyses showed that Mallomonas furtiva is closely related to M . kalinae , with which it formed a highly supported lineage. Distribution patterns of all three studied taxa are further discussed. 相似文献