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81.
E. A. Azizov A. V. Babarykin A. V. Voronin V. K. Gusev A. Yu. Malyshev V. K. Markov A. A. Petrov V. G. Petrov Yu. V. Petrov V. V. Rozhdestvenskii N. V. Sakharov 《Plasma Physics Reports》2004,30(5):422-431
A weeping pulsed radar reflectometer designed for measuring the spatial electron density distribution in the Globus-M spherical tokamak with a minor plasma radius of a=24 cm, a major radius of R=36 cm, a toroidal field of B T=0.5 T, a plasma current of I p=200 kA, and an average density of n=(3–10)×1013 cm?3 is described. The reflectometer operation is based on the reflection of microwaves with a carrier frequency f from a plasma layer with the critical density n=(0.0111f)2, where n is the electron density in units of 1014 cm?3 and f is the microwave frequency in GHz. By simultaneously probing the plasma at different frequencies, it is possible to recover the electron density profile. Microwave pulses with different frequencies are obtained by frequency sweeping. To increase the range of measured densities, channels with fixed frequencies are also used; as a result, the instrument has eleven frequency channels: a 19.5-GHz channel, eight channels in the 26-to 40-GHz frequency range, a 51.5-GHz channel, and a 60-GHz channel, which corresponds to eleven points in the density profile: 0.47×1013 cm?3, eight points in the (0.8–1.95)×1013-cm?3 range, 3.27×1013 cm?3, and 4.5×1013 cm?3. The reflectometer allows detailed measurements of the density profile with a time resolution of several tens of microseconds, which can be useful, in particular, in studying the processes related to the formation of an internal transport barrier in plasma. The first results obtained using this reflectometer in the Globus-M tokamak under various operating conditions are discussed. 相似文献
82.
几种脊椎动物宽频带心电图波形与心电向量环特点的比较 总被引:1,自引:0,他引:1
对小鼠、家鸽、蟾蜍、鲫鱼4种脊椎动物宽频带心电图Ⅱ导联的波形特点及QRS额面心电向量环的位置、形态特点进行了比较研究。结果发现,(1)宽频带心电图Ⅱ导联QRS波群小鼠、蟾蜍、鲫鱼QRS波群的主波均向上,而家鸽的主波向下;(2)QRS波群时程(ms)小鼠88±09,家鸽365±14,蟾蜍790±110,鲫鱼283±57;(3)QRS心电向量环的位置家鸽的位于-90°~-180°象限内,这是家鸽心电图Ⅱ导联QRS波群主波向下的根本原因;小鼠、蟾蜍、鲫鱼的均位于0°~90°象限内,与它们的QRS波群主波向上相一致;(4)QRS心电向量环的形状;小鼠的QRS向量环较其它3种动物的要大,蟾蜍的最小。鲫鱼的不规则,有的呈三角形,有的呈“8”字形,还有的呈半圆形,这是导致鲫鱼的QRS波群出现较多切迹和扭挫的原因。 相似文献
83.
Some properties of human small heat shock protein Hsp20 (HspB6). 总被引:2,自引:0,他引:2
Olesya V Bukach Alim S Seit-Nebi Steven B Marston Nikolai B Gusev 《European journal of biochemistry》2004,271(2):291-302
Human heat shock protein of apparent molecular mass 20 kDa (Hsp20) and its mutant, S16D, mimicking phosphorylation by cyclic nucleotide-dependent protein kinases, were cloned and expressed in Escherichia coli. The proteins were obtained in a homogeneous state without utilization of urea or detergents. On size exclusion chromatography at neutral pH, Hsp20 and its S16D mutant were eluted as symmetrical peaks with an apparent molecular mass of 55-60 kDa. Chemical crosslinking resulted in the formation of dimers with an apparent molecular mass of 42 kDa. At pH 6.0, Hsp20 and its S16D mutant dissociated, and were eluted in the form of two peaks with apparent molecular mass values of 45-50 and 28-30 kDa. At pH 7.0-7.5, the chaperone activity of Hsp20 (measured by its ability to prevent the reduction-induced aggregation of insulin or heat-induced aggregation of yeast alcohol dehydrogenase) was similar to or higher than that of commercial alpha-crystallin. Under these conditions, the S16D mutant of Hsp20 possessed lower chaperone activity than the wild-type protein. At pH 6.0, both alpha-crystallin and Hsp20 interacted with denatured alcohol dehydrogenase; however, alpha-crystallin prevented, whereas Hsp20 either did not affect or promoted, the heat-induced aggregation of alcohol dehydrogenase. The mixing of wild-type human Hsp27 and Hsp20 resulted in a slow, temperature-dependent formation of hetero-oligomeric complexes, with apparent molecular mass values of 100 and 300 kDa, which contained approximately equal amounts of Hsp27 and Hsp20 subunits. Phosphorylation of Hsp27 by mitogen activated protein kinase-activated protein kinase 2 was mimicked by replacing Ser15, 78 and 82 with Asp. A 3D mutant of Hsp27 mixed with Hsp20 rapidly formed a hetero-oligomeric complex with an apparent molecular mass of 100 kDa, containing approximately equal quantities of two small heat shock proteins. 相似文献
84.
对热原不合格的抗淋巴细胞免疫球蛋白 (ALG)半制品再制条件进行了研究。将高热原的ALG半制品通过使用碱、酸以及A5 0处理后 ,细菌内毒素的含量明显降低 ,热原质经家兔和鲎试剂检测均合格 ,其生物学效价仍不低于 1 :4 0 0 0 ,而且它们的其它各项主要指标也都达到中国生物制品暂行规程的要求。这一试验结果表明碱、酸处理并辅以A5 0吸附是去除ALG制品中热原的一种有效方法 相似文献
85.
南方鲇的营养学研究:饲料中大豆蛋白水平对消化率及摄食率的影响 总被引:14,自引:0,他引:14
以23.78±0.09g的南方鲇为实验对象,以Cr2O3作为测定饲料消化率的指示物,以大豆蛋白分别替代0%、13%、26%、39%、52%和65%的鱼粉蛋白,配制成6种等氮(粗蛋白48%)、等能(总能20KJ/g)的饲料,用于探讨饲料中大豆蛋白替代鱼粉蛋白的比例对南方鲇消化率及摄食率的影响。实验在27.5±0.02℃水温条件下进行6周,溶氧维持在5mg/L以上,光制为14L∶10D。结果表明,饲料中不同比例的鱼粉蛋白与大豆蛋白对南方鲇的消化率及摄食率均存在显著影响(P<0.05)。其中南方鲇对于物质、蛋白质、脂肪和能量的消化率随着大豆蛋白替代率的提高而逐步下降,当替代量为65%时,其消化率显著低于其余各组(P<0.05)。而南方鲇的摄食率均随着饲料中大豆粉添加量的升高而升高,当大豆蛋白替代鱼粉蛋白超过39%时,摄食率显著高于其余各组(P<0.05)。通过分析认为,消化率是限制南方鲇利用大豆粉的重要因素,而摄食率不是影响其利用率的主要原因。 相似文献
86.
G. P. Gusev A. O. Sherstobitov T. I. Ivanova A. Yu. Bogdanova 《Journal of Evolutionary Biochemistry and Physiology》2001,37(3):225-230
To study role of glycolysis and oxidative metabolism in providing active transport of monovalent cations, isolated erythrocytes of the lamprey Lampetra fluviatlis were incubated at 20°C in the presence of various metabolic inhibitors. The active (ouabain-sensitive) K+ (86Rb) influx into erythrocytes did not change after cell incubation for 1–2 h in the absence of glucose or in the presence of 10 mM deoxy-D-glucose or 1 mM monoiodoacetate. Inhibitors of oxidative phosphorylation (antimycin A, rotenone, sodium azide, cyanide) produced a significant decrease (on average, by 74% ) in the active K+ transport in the lamprey erythrocytes. All blockers of oxidative phosphorylation produced the same degree of inhibition of the K+ transport after the cell pre-incubation with them for 30 and 60 min. In experiments with rotenone, the K+ influx was reduced statistically significantly as early as in 5 min of cell incubation and reached a maximal effect after 10–20 min. The intracellular ATP content in erythrocytes decreased by 17, 37, and 45% after 5, 10, and 20 min of cell incubation with rotenone, respectively. The active K+ transport in the lamprey erythrocytes is most likely to be closely associated with the intracellular ATP concentration. The data obtained indicate that the energy supply of the Na,K-pump in the lamprey erythrocytes is due exclusively to oxidative phosphorylation processes. 相似文献
87.
The infection of tobacco, nightshade, rice plants, and their tissue cultures with the cyanobacteria–bacteria associative microsymbiont complexes (AMC) isolated from natural syncyanoses (the ferns Azolla pinnataand Azollasp. and the cycad Encephalartos ferox) was studied. The inoculation of the intact plants or their cuttings with AMC led to the colonization of the plant roots, stems, and leaves by cyanobacteria and their bacterial symbionts (referred to as satellite bacteria, SB). The sites of the long-term contact of plant organs with cyanobacteria were characterized by the formation of copious slime. On the roots of infected plants, one could observe the callus growth of cortical parenchyma cells and the formation of pseudonodules, in which SB cells gradually accumulated. In mixed cultures of plant callus tissues and the AMC isolated from the fernsA. pinnataand Azollasp., the callus tissue specifically influenced the growth of the AMC components, causing (depending on the plant species and strain) either their balanced growth, or their cyclic growth, or the predominant growth of one of the AMC components (either cyanobacteria or satellite bacteria). This phenomenon is proposed to be used for the dissociation of stable multicomponent natural symbiotic complexes and the selection of their particular components. 相似文献
88.
报道了鲎试剂在抗淋巴细胞免疫球蛋白内毒素检查中的应用。在用鲎试剂检测细菌内毒素时 ,按中国药典的要求对鲎试剂灵敏度进行标定 ,并作干扰实验 (增强或抑制实验 ) ,然后用鲎试法与家兔法同时测定 15批抗淋巴细胞免疫球蛋白。结果发现三批鲎试剂的灵敏度标示值均正确 ,五批抗淋巴细胞免疫球蛋白半成品在最大有效稀释度 (MVD)时对试验无干扰 ,这两种方法测定结果的符合率达到 91.7%。从而表明鲎试法很有可能代替家兔法 相似文献
89.
90.
Phosphorylation of smooth muscle caldesmon by three protein kinases: implication for domain mapping 总被引:1,自引:0,他引:1
Phosphorylation of duck gizzard caldesmon by Ca2+/phospholipid-dependent protein kinase, Ca2+/calmodulin-dependent protein kinase and casein kinase II has been investigated. The Ca2+/phospholipid-dependent protein kinase incorporates more than 3 mol phosphate per mol (140 kDa) caldesmon. All phosphorylation sites are localized in the actin- and calmodulin-binding peptide (40-45 kDa) supposed to be a part of the C-terminal domain of caldesmon. Casein kinase II phosphorylates only one site located in a short (25-27 kDa) peptide, presumably in the caldesmon N-terminal domain. The Ca2+/calmodulin-dependent protein kinase phosphorylates two sites located in the N- and C-terminal domains of caldesmon. 相似文献