全文获取类型
收费全文 | 381篇 |
免费 | 23篇 |
出版年
2022年 | 4篇 |
2021年 | 9篇 |
2020年 | 6篇 |
2019年 | 2篇 |
2018年 | 6篇 |
2017年 | 4篇 |
2016年 | 17篇 |
2015年 | 18篇 |
2014年 | 25篇 |
2013年 | 30篇 |
2012年 | 34篇 |
2011年 | 34篇 |
2010年 | 17篇 |
2009年 | 23篇 |
2008年 | 26篇 |
2007年 | 23篇 |
2006年 | 19篇 |
2005年 | 17篇 |
2004年 | 12篇 |
2003年 | 12篇 |
2002年 | 13篇 |
2001年 | 5篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1998年 | 8篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1983年 | 2篇 |
1981年 | 1篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1967年 | 1篇 |
1957年 | 2篇 |
1943年 | 1篇 |
1941年 | 1篇 |
排序方式: 共有404条查询结果,搜索用时 15 毫秒
81.
82.
83.
Microdissection of Shoot Meristem Functional Domains 总被引:1,自引:0,他引:1
84.
P58IPK: A Novel “CIHD” Member of the Host Innate Defense Response against Pathogenic Virus Infection
85.
86.
Cell-penetrating peptides comprising cloned epitopes that contribute to membrane transduction, DNA-binding and cell targeting
functions are known to facilitate nucleic acid delivery. Using the ITASSER software, we predicted the 3-D structure of a well
characterized and efficient transfecting cell-penetrating peptide, namely TAT-Mu and its derivative TAT-Mu-AF protein that
harbors a targeting ligand, the HER2-binding affibody. Our model predicts TAT-Mu-AF fusion protein as primarily comprising
α-helices. The affibody in TAT-Mu-AF is predicted as a 3-helical domain that is distinct from the TAT-Mu domain. Its positioning
in three-dimensional structure is oriented in a manner that possibly favors interactions with receptor and facilitates transport
to the target site. The linker region between TAT-Mu and the affibody is also predicted as a helix that is likely to stabilize
the overall fold of the TAT-Mu-AF complex. Further, the evaluation of secondary structure of the designed TAT-Mu-AF fusion
protein by circular dichroism is in support of our predictions. 相似文献
87.
A novel 13 residue acyclic peptide from the marine snail, Conus monile, targets potassium channels 总被引:1,自引:0,他引:1
Sudarslal S Singaravadivelan G Ramasamy P Ananda K Sarma SP Sikdar SK Krishnan KS Balaram P 《Biochemical and biophysical research communications》2004,317(3):682-688
A novel 13-residue peptide Mo1659 has been isolated from the venom of a vermivorous cone snail, Conus monile. HPLC fractions of the venom extract yielded an intense UV absorbing fraction with a mass of 1659Da. De novo sequencing using both matrix assisted laser desorption and ionization and electrospray MS/MS methods together with analysis of proteolytic fragments successfully yielded the amino acid sequence, FHGGSWYRFPWGY-NH(2). This was further confirmed by comparison with the chemically synthesized peptide and by conventional Edman sequencing. Mo1659 has an unusual sequence with a preponderance of aromatic residues and the absence of apolar, aliphatic residues like Ala, Val, Leu, and Ile. Mo1659 has no disulfide bridges distinguishing it from the conotoxins and bears no sequence similarity with any of the acyclic peptides isolated thus far from the venom of cone snails. Electrophysiological studies on the effect of Mo1659 on measured currents in dorsal root ganglion neurons suggest that the peptide targets non-inactivating voltage-dependent potassium channels. 相似文献
88.
89.
We propose three-dimensional models corresponding to the C-terminal domain of human alphaA- and alphaB-crystallins by using the comparative modeling program Modeler and the more closely related crystal structure of the small heat-shock protein (sHSP) belonging to the eukaryotic species from wheat HSP16.9 as template structure. The sequence alignments differ slightly from alignments that were used previously to construct alpha-crystallin models based on homology and the crystal structure of the more distantly related small heat-shock protein from archaeal species; Methanococcus jannaschii Mj HSP16.5, the only related structure then available as a template. The alpha-crystallin models based on HSP16.9 show better 3-D profile scores and reflect the relative shifts in the beta-strands corresponding to the beta-sandwich associated with the core C-terminal domain that is common to small heat-shock proteins and the alpha-crystallins. The loop between the equivalent beta5-beta7 strands corresponds to a region of seven amino acid residues deletion in alpha-crystallins and defines the new set of amino acid residues likely to be associated with a dimer interface. The models may be useful to examine sites of mutations that are known to affect chaperone-like activity and provide the structural basis for dimerization in alpha-crystallins. 相似文献
90.
Pichyangkul S Endy TP Kalayanarooj S Nisalak A Yongvanitchit K Green S Rothman AL Ennis FA Libraty DH 《Journal of immunology (Baltimore, Md. : 1950)》2003,171(10):5571-5578
At least two distinct human dendritic cell (DC) subsets are produced in the bone marrow and circulate in the peripheral blood-precursor myeloid DCs (pre-mDCs) and plasmacytoid DCs (PDCs). Both lineages of DCs are instrumental in antiviral innate immunity and shaping Th1 adaptive immune responses. PDCs are the most potent IFN-alpha-producing cells to viral pathogens. Dengue, an acute flavivirus disease, provides a model to study DC responses to a self-limited human viral infection. We analyzed circulating DC subsets in a prospective study of children with dengue across a broad range of illness severities: healthy controls; mild, nondengue, presumed viral infections; moderately ill dengue fever; and, the most severe form of illness, dengue hemorrhagic fever. We also examined PDC responses in monkeys with asymptomatic dengue viremia and to dengue virus exposure in vitro. The absolute number and frequency of circulating pre-mDCs early in acute viral illness decreased as illness severity increased. Depressed pre-mDC blood levels appeared to be part of the typical innate immune response to acute viral infection. The frequency of circulating PDCs trended upward and the absolute number of circulating PDCs remained stable early in moderately ill children with dengue fever, mild other, nondengue, febrile illness, and monkeys with asymptomatic dengue viremia. However, there was an early decrease in circulating PDC levels in children who subsequently developed dengue hemorrhagic fever. A blunted blood PDC response to dengue virus infection was associated with higher viremia levels, and was part of an altered innate immune response and pathogenetic cascade leading to severe disease. 相似文献