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191.
G. Franklin L. Carpenter E. Davis C.S. Reddy D. Al-Abed W. Abou Alaiwi M. Parani B. Smith R.V. Sairam 《Plant Growth Regulation》2004,43(1):73-79
Soybean (Glycine max L.) plantlets were e?ciently regenerated from mature and immature cotyledons of?ve different genotypes by studying various parameters affecting regeneration. Green organogenic noduleswere induced at the proximal end, which subsequently differentiated into shoot buds on modi?ed MS(Murashige T. and Skoog F. 1962. Physiol. Plant. 15: 473-497) medium. The presence of 6-benzylaminopurine(BAP) and thidiazuron (TDZ) in the medium exerted a synergistic effect, in that regenerationeffciency was higher than for either cytokin alone. The regenerated shoot buds elongated and rooted onMS medium containing 0.29 lM gibberellic acid (GA3) and 2.69 lM a-naphthaleneaceticacid (NAA),respectively. Rooted plants were established in the greenhouse with 87% success and produced viable seeds.Preliminary studies with Agrobacterium show great promise for soybean transformation based on theregeneration protocol reported here. 相似文献
192.
193.
M.R. Sairam 《Biochimica et Biophysica Acta (BBA)/General Subjects》1982,717(1):149-153
Bovine lutropin was successfully deglycosylated by treatment with anhydrous HF at 0°C for 60 min. The overall loss of carbohydrate residues was about 70%. Fucose, mannose and galactosamine residues were removed completely. About six residues of N-acetylglucosamine were left in the deglycosylated hormone. This degree of deglycosylation did not reduce receptor binding ability but in vitro biological activity was completely lost. Consistent with these properties, the deglycosylated lutropin inhibited the in vitro biological action of the native lutropin. The results demonstrate that the full complement of carbohydrate residues including the sulfated hexosamines are not required for receptor binding but they are necessary to impart appropriate conformational features necessary for activation of the target cells. 相似文献
194.
The disulfide linkage of the 12 half-cystine residues in the beta subunit of ovine interstitial cell stimulating hormone has been investigated by enzymic and partial acid hydrolysis of the intact molecule. Results indicate that the disulfide bridges are formed by residues 9-38, 23-72, 26-110, 34-90, 57-88, and 93-100. 相似文献
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196.
Termination of pregnancy in the rat by the antiserum to the beta subunit of ovine interstitial cell stimulating hormone 总被引:1,自引:0,他引:1
197.
The disulfide linkage of the ten half-cystine residues in the α-subunit of ovine interstitial cell-stimulating hormone has been investigated by partial acid and enzymic hydrolysis of the intact molecule. Results indicate that the disulfide bridges are formed by residues 14–86, 11–64, 35–63, 32–89, and 36–91. 相似文献
198.
Lihua Ye Munhyung Bae Chelsi D. Cassilly Sairam V. Jabba Daniel W. Thorpe Alyce M. Martin Hsiu-Yi Lu Jinhu Wang John D. Thompson Colin R. Lickwar Kenneth D. Poss Damien J. Keating Sven-Eric Jordt Jon Clardy Rodger A. Liddle John F. Rawls 《Cell host & microbe》2021,29(2):179-196.e9
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199.