Comparsion of staining characteristics of Toto bodies

Toto bodies are eosinophilic structures that resemble the cells of the superficial cell layer of the oral epithelium. Toto bodies commonly are associated with inflammatory gingival and other mucosal lesions including pyogenic granuloma, irritational fibroma, epulis fissuratum, peripheral giant cell granuloma and inflammatory hyperplastic gingivitis. We evaluated staining characteristics of Toto bodies to establish their origin and to identify their significance in lesions. We investigated pyogenic granuloma, fibroma and leukoplakia with epithelium that exhibited Toto bodies after hematoxylin and eosin (staining. Sections were stained with Alcian blue, periodic acid-Schiff and Ayoub-Shklar stains to evaluate staining intensity and distribution. More Toto bodies were found in pyogenic granuloma than in fibroma and leukoplakia. PAS and Alcian blue staining exhibited mild intensity and did not establish the origin of Toto bodies. High staining intensity and diffuse distribution of stain was observed using Ayoub-Shklar staining, which indicated that Toto bodies originate from keratin.     

Regulation of in vitro progesterone release from caprine luteal tissues by prostaglandins E2 and F2a

Luteal slices obtained from Day-10 cyclic, sexually mature, mixed-breed, superovulated goats were used to study the effects of prostaglandins E(2) and F(2)a (PGE(2) and PGF(2)a) on the release of progesterone. The goats were synchronized for estrus using a single intramuscular injection of 5 mg PGF(2)a given during the mid-luteal phase of the estrous cycle. Multiple follicular growth and superovulation were induced using a treatment regiment of follicle stimulating hormone (FSH) and luteinizing hormone releasing hormone (LHRH) previously standardized in our laboratory (1). The luteal slices were treated with PGE(2) or PGF(2)a at concentrations of 1 and 10 ng/ml each. Untreated luteal slices continued to release significant amounts of progesterone over the entire period of incubation (30 to 360 minutes). There was a progressive increase in progesterone accumulation following treatment with PGE(2) at both concentrations. The mean progesterone values were significantly higher in the PGE(2)-treated groups at all incubation periods than in the controls. Progesterone values at 10 ng/ml were higher (P<0.05) than at 1 ng/ml. Treatment with PGF(2)a decreased (P<0.05) progesterone release at 60 to 360 minutes of incubation compared with that of the corresponding controls for each incubation period. However, there appeared to be no differences (P>0.05) in mean progesterone values between the two concentrations of PGF(2)a. The results of this study showed that PGE(2) enhanced the release of progesterone by caprine luteal tissues, whereas PGF(2)a inhibited its release.     

Evaluation of the prevalence and economic burden of adverse drug reactions presenting to the medical emergency department of a tertiary referral centre: a prospective study

Background  

Adverse drug reactions (ADRs) are now recognized as an important cause of hospital admissions, with a proportion ranging from 0.9–7.9%. They also constitute a significant economic burden. We thus aimed at determining the prevalence and the economic burden of ADRs presenting to Medical Emergency Department (ED) of a tertiary referral center in India     

鼠李糖脂的表面化学和生物合成及其在垃圾堆肥中的应用展望

鼠李糖脂是生物表面活性剂中一类非常重要而应用广泛的微生物发酵产物,在环境污染修复中需求量越来越多。针对近十年来国内外对鼠李糖脂生物表面活性剂的研究,较系统地总结了其化学结构、性质、生物合成机理及产量调节方法,及大规模生产鼠李糖脂的基础研究工作,并对其在城市生活垃圾堆肥中的应用做了展望。