Cloning and Drought Resistance Analysis of Soybean <i>GmHsps_p23-like</i> Gene

Soybean (Glycine max (L.) Merr.) is an important cultivated crop, which requires much water during its growth, and drought seriously affects soybean yields. Studies have shown that the expression of small heat shock proteins can enhance drought resistance, cold resistance and salt resistance of plants. In this experiment, soybean GmHsps_p23-like gene was successfully cloned by RT-PCR, the protein encoded by the GmHsps_p23-like gene was subjected to bioinformatics analysis, and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed. Agrobacterium-mediated method was used to transform soybeans to obtain positive plants. RT-PCR detection, rehydration experiment and drought resistance physiological and biochemical index detection were performed on the T₂ generation positive transgenic soybean plants identified by PCR and Southern hybridization. The results showed that the overexpression vector plant GmHsps_p23-like gene expression increased. After rehydration, the transgenic overexpression plants returned to normal growth, and the damage to the plants was low. After drought stress, the SOD and POD activities and the PRO content of the transgenic overexpression plants increased, while the MDA content decreased. The reverse was true for soybean plants with genetically modified editing vectors. The drought resistance of the overexpressed soybeans under drought stress was higher than that of the control group, and had a stronger drought resistance. It showed that the expression of soybean GmHsps_p23-like gene can improve the drought resistance of soybean. The cloning and functional verification of soybean GmHsps_p23-like gene had not been reported yet. This is the first time that PCR technology has been used to amplify the soybean GmHsps_p23-like gene and construct an expression vector for this gene. This research has laid the foundation for transgenic technology to improve plant drought resistance and cultivate new drought-resistant transgenic soybean varieties.     

Comprehensive insights into the influence of supplemental green light on the photosynthesis of ginger (Zingiber officinale Roscoe)

Although green light is not considered to contribute to the photosynthesis of plants, the photosynthesis of ginger, a dual-purpose vegetable used as a medicine and food, is affected by the green wave band. In this study, the supplementary green band of sunlight (SG) increased the net photosynthetic rate (Pn), maximal photochemical efficiency of PSII (Fv/Fm), and actual photochemical efficiency of PSII (Y(II)) compared with the sunlight treatment (S). The Pn and Fv/Fm of the SG treatment were higher than those of the white light (W) treatment, while the Pn and Fv/Fm of the green light (G) treatment alone were lower than those of the W treatment. Further analysis found that the minimal fluorescence (Fo) of the S treatment increased, especially at noon, while the Fo of the SG treatment decreased. Similarly, the Fo of the W treatment increased significantly, while the Fo of the white–green mixed light (WG) treatment decreased. The relative fluorescence values of the K-J-I bands in the SG and WG treatments were lower than those in the S and W treatments, respectively. The photochemical quenching (qP) of the WG treatment was higher than that of the W treatment, while the primary thermal losses corresponded to the sum of nonregulated heat dissipation and fluorescence emission (Y(NO)) of the WG treatment was lower than that of the W treatment. The SG treatment reduced the accumulation of plastoglobules but increased the accumulation of starch granules and leaf thickness. Moreover, the green band supplemented with white light significantly increased the biomass of the aboveground plant parts and promoted the active growth of the aboveground parts. Supplementing green light plays a regulatory role in ginger based on the following four points. First, it effectively promotes the transfer of electrons between the acceptor side of photosystem II; second, it optimizes ginger photosynthesis; third, it alleviates strong light stress by reducing the accumulation of reactive oxygen species; and fourth, it promotes heat dissipation and reduces the rapid burst of active oxygen in the chloroplast caused by excess energy. In summary, green light can significantly optimize the photosynthetic characteristics of ginger.

     

The mechanisms for the difference in waterlogging tolerance among sea barley,wheat and barley

Plant Growth Regulation - Waterlogging is a major abiotic stress limiting crop growth and yield. There is a distinct difference among cereal crops in their responses to waterlogging stress,...     

RNAi介导的GdHsp60和GdHsp70基因沉默对沙葱萤叶甲幼虫抗寒性的影响

【目的】本研究旨在比较不同RNAi方法对沙葱萤叶甲Galeruca daurica幼虫热激蛋白基因(GdHsp60和GdHsp70)的沉默效率,以选择一种可高效降低靶基因表达水平的研究方法,明确这两个热激蛋白在沙葱萤叶甲幼虫抗寒性中的作用。【方法】分别通过饲喂法和显微注射法进行RNAi沉默沙葱萤叶甲1和2龄幼虫的GdHsp60和GdHsp70,采用qPCR检测GdHsp60和GdHsp70的沉默效率;通过显微注射法分别 对GdHsp60和GdHsp70进行RNAi后24 h,用热电偶法测定沙葱萤叶甲2龄幼虫过冷却点和结冰点,生物测定沙葱萤叶甲2龄幼虫暴露于不同低温条件下(-6~-14℃) 2 h的半致死温度(Ltemp₅₀)以及在-5℃低温条件下处理不同时间后的半致死时间(Ltime₅₀)。【结果】用饲喂法和显微注射法进行RNAi均可以降低GdHsp60和GdHsp70的表达水平,但显微注射法的沉默效率更高。与对照组(显微注射dsGFP)相比,沙葱萤叶甲2龄幼虫分别显微注射dsGdHsp60和dsGdHsp70 24 h后,GdHsp60和GdHsp70的表达水平均降至最低,分别降低了84.15%和92.38%。在沙葱萤叶甲2龄幼虫中,显微注射dsGdHsp60 24 h后其过冷却点、结冰点、Ltemp₅₀及Ltime₅₀值分别-10.56±0.42℃,-7.66±0.56℃,-8.33℃和49.25 h,显微注射dsGdHsp70 24 h后其过冷却点、结冰点、Ltemp₅₀及Ltime₅₀值分别为-9.08±0.23℃,-6.09±0.28℃, -8.20℃和52.21 h,而对照组的分别为-14.71±0.11℃,-13.94±0.09℃,-10.63℃和87.13 h。与对 照组(显微注射dsGFP)相比,在沙葱萤叶甲2龄幼虫分别显微注射dsGdHsp60和dsGdHsp70 24 h后过冷却点 、结冰点和Ltemp₅₀显著上升,而Ltime50值显著缩短。【结论】显微注射法可作为沙葱萤叶甲Hsp相关基 因RNAi的主要干扰方法;沉默GdHsp60和GdHsp70基因均会显著降低了沙葱萤叶甲幼虫的抗寒能力。     

Plant Hormone Metabolome and Transcriptome Analysis of Dwarf and Wild-type Banana

Journal of Plant Growth Regulation - Gibberellin (GA), auxin (IAA) and brassinosteroid (BR) are indispensable in the process of plant growth and development. Currently, research on the regulatory...     

PLEK2 promotes the proliferation and migration of non-small cell lung cancer cells in a BRD4-dependent manner

Molecular Biology Reports - It has been reported that Pleckstrin 2 (PLEK2) acts as an oncogene in non-small cell lung cancer (NSCLC). Bromodomain containing protein 4 (BRD4), an important...     

Exosomal miR-19a decreases insulin production by targeting Neurod1 in pancreatic cancer associated diabetes

Molecular Biology Reports - New onset diabetes mellitus demonstrates a roughly correlation with pancreatic cancer (PaC), which is unique in PaC and was named as PaC-induced DM, but the inner...     

Curcumin Attenuates the PERK-eIF2α Signaling to Relieve Acrylamide-Induced Neurotoxicity in SH‑SY5Y Neuroblastoma Cells

Neurochemical Research - Curcumin is a natural polyphenolic compound with neuroprotective and antioxidant properties. Acrylamide (ACR) is a by-product of food processing that produces neurotoxicity...     

缙云山4种林分土壤无机磷与活性铝的含量及分布

为探索不同林分对土壤中无机磷与活性铝的含量及分布的影响,以及无机磷与活性铝之间的相互关系,以在我国西南地区酸性土壤上的农林经营管理提供理论和实践依据。研究以缙云山广泛分布的4种林分类型：山莓、马尾松、楠竹和柑橘林土壤为对象,采用酸性土壤无机磷分级方法和铝试剂比色法测定了土壤剖面A、B和C层中各形态的无机磷与活性铝的含量,分析了不同林分土壤中无机磷和活性铝的含量和分布特征。结果表明,林分类型显著影响土壤中无机磷与活性铝的含量与分布,且铁磷(Fe-P)、交换态铝(Ex-Al)和羟基铝(Hy-Al)的含量和比例还受到土壤层次的影响。4种林分相比,山莓林能促进闭蓄态磷(O-P)的形成,而马尾松、楠竹和柑橘林则有利于Al-P、Fe-P和Ca-P的形成;柑橘林有利于低活性的腐殖酸铝(Al-AH)形成,而山莓、马尾松和楠竹林促进高活性的Ex-Al或Hy-Al溶出;Ex-Al、Al-P和Fe-P在土层的分布上有表层富集现象,而Hy-Al集中分布于B层。此外,土壤Ex-Al和Al-P与Fe-P之间,Hy-Al和Ca-P之间均呈显著(P<0.05)正相关。因此,林分类型显著影响土壤无机磷与活性铝的含...     

2013-2018年武夷山亚热带常绿阔叶林乔木层动态

从植物群落组成和结构的角度研究森林群落的演替规律,是探究森林群落的退化与恢复过程及相关机制的重要途径,对于指导天然林保护和恢复具有重要理论价值和实践意义。2018年,对面积为9.6 hm~2的武夷山亚热带常绿阔叶林样地进行了第2轮调查,并从重要值、物种多度、物种丰富度、Shannon-Wiener多样性指数、Simpson指数、Margalef丰富度指数、Pielou均匀度指数、死亡率、补员率、种群大小变化率、相对适合度、胸径变异系数等角度分析了乔木层群落动态。结果表明：2018年的乔木个体为48科88属174种,较2013年增加了1种。Margalef丰富度指数上升,Shannon-Wiener多样性指数、Simpson指数、Pielou均匀度指数均略有下降。DBH≥1 cm的乔木个体从68336个减少到63897个,共死亡7430个个体,补员2991个个体。群落的年死亡率为2.30%,年补员率为0.96%,种群大小变化率为-1.34%,相对适合度为0.42。群落的平均胸径从5.02(±0.02) cm上升为5.49(±0.03) cm。28个重要值之和由71.92%下降为71.81...