全文获取类型
收费全文 | 19343篇 |
免费 | 1771篇 |
国内免费 | 2127篇 |
出版年
2024年 | 40篇 |
2023年 | 256篇 |
2022年 | 555篇 |
2021年 | 976篇 |
2020年 | 715篇 |
2019年 | 859篇 |
2018年 | 885篇 |
2017年 | 588篇 |
2016年 | 810篇 |
2015年 | 1245篇 |
2014年 | 1521篇 |
2013年 | 1579篇 |
2012年 | 1895篇 |
2011年 | 1709篇 |
2010年 | 1100篇 |
2009年 | 1048篇 |
2008年 | 1117篇 |
2007年 | 1052篇 |
2006年 | 860篇 |
2005年 | 706篇 |
2004年 | 632篇 |
2003年 | 543篇 |
2002年 | 461篇 |
2001年 | 309篇 |
2000年 | 295篇 |
1999年 | 269篇 |
1998年 | 188篇 |
1997年 | 154篇 |
1996年 | 144篇 |
1995年 | 96篇 |
1994年 | 122篇 |
1993年 | 66篇 |
1992年 | 86篇 |
1991年 | 68篇 |
1990年 | 61篇 |
1989年 | 40篇 |
1988年 | 34篇 |
1987年 | 21篇 |
1986年 | 16篇 |
1985年 | 25篇 |
1984年 | 13篇 |
1983年 | 16篇 |
1982年 | 15篇 |
1980年 | 4篇 |
1978年 | 5篇 |
1974年 | 3篇 |
1973年 | 5篇 |
1972年 | 3篇 |
1968年 | 3篇 |
1965年 | 8篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
951.
Developmental expression and stress induction of glutathione S-transferase in the spruce budworm, Choristoneura fumiferana 总被引:1,自引:0,他引:1
Feng Q Davey KG S D Pang A Ladd TR Retnakaran A Tomkins BL Zheng S Palli SR 《Journal of insect physiology》2001,47(1):1-10
Developmental and stress-induced expression of Choristoneura fumiferana glutathione S-transferase (CfGST) mRNA and protein were examined using Northern blots and Western blots. High levels of CfGST mRNA and protein were detected in 1st instar larvae and diapausing 2nd instar larvae. Expression of CfGST gradually decreased during larval development from 3rd to 5th instar, after which the expression increased once again, reaching peak levels in 6th instar larvae. CfGST mRNA and protein were undetectable in the pupal stage. Exposure to low temperature did not induce an increase in CfGST expression. Feeding on balsam fir foliage resulted in an increase in the expression of CfGST as compared to larvae that fed on artificial diet. The bacterial insecticide, Bacillus thuringiensis delta-endotoxin (Bt), the non-steroidal ecdysone analog, tebufenozide, and the synthetic pyrethroid, permethrin, induced the expression of CfGST mRNA in 5th instar larvae, whereas the chitin synthesis inhibitor, diflubenzuron, did not have any such effect. These results suggest that CfGST plays an important role in detoxifying various allelochemicals and insecticides in the spruce budworm. The developmental expression pattern strongly suggests that in addition to detoxification, CfGST might be involved in other functions. 相似文献
952.
Feng Y Minnerly JC Zurfluh LL Joy WD Hood WF Abegg AL Grabbe ES Shieh JJ Thurman TL McKearn JP McWherter CA 《Biochemistry》1999,38(14):4553-4563
The sequence of granulocyte colony-stimulating factor (G-CSF) has been circularly permuted by introducing new chain termini into interhelical loops and by constraining the N- and C-terminal helices, either by direct linkage of the termini (L0) or by substitution of the amino-terminal 10-residue segment with a seven-residue linker composed of glycines and serines (L1). All the circularly permuted G-CSFs (cpG-CSFs) were able to fold into biologically active structures that could recognize the G-CSF receptor. CD and NMR spectroscopy demonstrated that all of the cpG-CSFs adopted a fold similar to that of the native molecule, except for one [cpG-CSF(L1)[142/141]] which has the new termini at the end of loop 34 with the shorter L1 linker. All of the cpG-CSFs underwent cooperative unfolding by urea, and a systematically lower free energy change (DeltaGurea) was observed for molecules with the shorter L1 linker than for those molecules in which the original termini were directly linked (the L0 linker). The thermodynamic stability of the cpG-CSFs toward urea was found to correlate with their relative ability to stimulate proliferation of G-CSF responsive cells. Taken together, these results indicate that the G-CSF sequence is robust in its ability to undergo linear rearrangement and adopt a biologically active conformation. The choice of linker, with its effect on stability, seems to be important for realizing the full biological activity of the three-dimensional structure. The breakpoint and linker together are the ultimate determinants of the structural and biological profiles of these circularly permuted cytokines. In the following paper [McWherter, C. A., et al. (1999) Biochemistry 38, 4564-4571], McWherter and co-workers have used circularly permuted G-CSF sequences to engineer chimeric dual IL-3 and G-CSF receptor agonists in which the relative spatial orientation of the receptor agonist domains is varied. Interpreting the differences in activity for the chimeric molecules in terms of the connectivity between domains depends critically on the results reported here for the isolated cpG-CSF domains. 相似文献
953.
954.
955.
956.
R S Harris G Feng K J Ross R Sidhu C Thulin S Longerich S K Szigety P J Hastings M E Winkler S M Rosenberg 《Mutation research》1999,437(1):51-60
This paper is an invited Response to a recent Commentary [P.L. Foster, Rev. Mut. Res. 436 (1999) 179-184] entitled "Are adaptive mutations due to a decline in mismatch repair? The evidence is lacking". The Commentary argues that no evidence exists supporting the idea that mismatch repair is limiting specifically during stationary-phase mutation. A primary concern of the author is to question the method that we used previously to measure growth-dependent mutation. In this method, mutation rates are calculated using counts of mutant colonies taken at times when those colonies arise, rather than at a predetermined, fixed time. Here we show further data that illustrate why this must be done to ensure accurate mutation measurements. Such accuracy was necessary for our published determination that mismatch repair proteins are not limiting during growth-dependent mutation, but become so during stationary-phase mutation. We review the evidence supporting the idea that stationary-phase reversion of a lac frameshift mutation occurs in an environment of decreased mismatch repair capacity. Those data are substantial. The data presented in the Commentary, in apparent contradiction to this idea, do not justify the conclusion presented there. 相似文献
957.
958.
959.
960.
The INK4 family of cyclin-dependent kinase (CDK) inhibitors negatively regulates cyclin D-dependent CDK4 and CDK6 and induces the growth-suppressive function of Rb family proteins. Mutations in the Cdk4 gene conferring INK4 resistance are associated with familial and sporadic melanoma in humans and result in a wide spectrum of tumors in mice, suggesting that INK4 is a major regulator of CDK4. Mice lacking the Cdk4 gene exhibit various defects in many organs associated with hypocellularity, whereas loss of the p18(Ink4c) gene results in widespread hyperplasia and organomegaly. To genetically test the notion that the function of INK4 is dependent on CDK4, we generated p18; Cdk4 double-mutant mice and examined the organs and tissues which developed abnormalities when either gene is deleted. We show here that, in all organs we have examined, including pituitary, testis, pancreas, kidney, and adrenal gland, hyperproliferative phenotypes associated with p18 loss were canceled. The double-mutant mice exhibited phenotypes very close to or indistinguishable from that of Cdk4 single-mutant mice. Mice lacking p27(Kip1) develop widespread hyperplasia and organomegaly similar to those developed by p18-deficient mice. The p27; Cdk4 double-mutant mice, however, displayed phenotypes intermediate between those of p27 and Cdk4 single-mutant mice. These results provide genetic evidence that in mice p18(Ink4c) and p27(Kip1) mediate the transduction of different cell growth and proliferation signals to CDK4 and that p18(Ink4c) is functionally dependent on CDK4. 相似文献