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991.
Kine Ø. Hanssen Gunnar Cervin Rozenn Trepos Julie Petitbois Tor Haug Espen Hansen Jeanette H. Andersen Henrik Pavia Claire Hellio Johan Svenson 《Marine biotechnology (New York, N.Y.)》2014,16(6):684-694
The inhibition of marine biofouling by the bromotyrosine derivative ianthelline, isolated from the Arctic marine sponge Stryphnus fortis, is described. All major stages of the fouling process are investigated. The effect of ianthelline on adhesion and growth of marine bacteria and microalgae is tested to investigate its influence on the initial microfouling process comparing with the known marine antifoulant barettin as a reference. Macrofouling is studied via barnacle (Balanus improvisus) settlement assays and blue mussel (Mytilus edulis) phenoloxidase inhibition. Ianthelline is shown to inhibit both marine micro- and macrofoulers with a pronounced effect on marine bacteria (minimum inhibitory concentration (MIC) values 0.1–10 μg/mL) and barnacle larval settlement (IC50?=?3.0 μg/mL). Moderate effects are recorded on M. edulis (IC50?=?45.2 μg/mL) and microalgae, where growth is more affected than surface adhesion. The effect of ianthelline is also investigated against human pathogenic bacteria. Ianthelline displayed low micromolar MIC values against several bacterial strains, both Gram positive and Gram negative, down to 2.5 μg/mL. In summary, the effect of ianthelline on 20 different representative marine antifouling organisms and seven human pathogenic bacterial strains is presented. 相似文献
992.
993.
Characterisation of culture-independent and -dependent microbial communities in a high-temperature offshore chalk petroleum reservoir 总被引:1,自引:0,他引:1
Krista M. Kaster Kristin Bonaunet Harald Berland Grethe Kjeilen-Eilertsen Odd Gunnar Brakstad 《Antonie van Leeuwenhoek》2009,96(4):423-439
Recent studies have indicated that oil reservoirs harbour diverse microbial communities. Culture-dependent and culture-independent
methods were used to evaluate the microbial diversity in produced water samples of the Ekofisk oil field, a high temperature,
and fractured chalk reservoir in the North Sea. DGGE analyses of 16S rRNA gene fragments were used to assess the microbial
diversity of both archaeal and bacterial communities in produced water samples and enrichment cultures from 4 different wells
(B-08, X-08, X-18 and X-25). Low diversity communities were found when 16S rDNA libraries of bacterial and archaeal assemblages
were generated from total community DNA obtained from produced water samples and enrichment cultures. Sequence analysis of
the clones indicated close matches to microbes associated with high-temperature oil reservoirs or other similar environments.
Sequences were found to be similar to members of the genera Thermotoga, Caminicella, Thermoanaerobacter, Archaeoglobus, Thermococcus, and Methanobulbus. Enrichment cultures obtained from the produced water samples were dominated by sheathed rods. Sequence analyses of the cultures
indicated predominance of the genera Petrotoga, Arcobacter, Archaeoglobus and Thermococcus. The communities of both produced water and enrichment cultures appeared to be dominated by thermophilic fermenters capable
of reducing sulphur compounds. These results suggest that the biochemical processes in the Ekofisk chalk reservoir are similar
to those observed in high-temperature sandstone reservoirs. 相似文献
994.
Aim The biogeographical patterns and drivers of diversity on oceanic islands in the tropical South Pacific (TSP) are synthesized. We use published studies to determine present patterns of diversity on TSP islands, the likely sources of the biota on these islands and how the islands were colonized. We also investigate the effect of extinctions. Location We focus on oceanic islands in the TSP. Methods We review available literature and published molecular studies. Results Examples of typical island features (e.g. gigantism, flightlessness, gender dimorphism) are common, as are adaptive radiations. Diversity decreases with increasing isolation from mainland sources and with decreasing size and age of archipelagos, corresponding well with island biogeographical expectations. Molecular studies support New Guinea/Malesia, New Caledonia and Australia as major source areas for the Pacific biota. Numerous studies support dispersal‐based scenarios, either over several 100 km (long‐distance dispersal) or over shorter distances by island‐hopping (stepping stones) and transport by human means (hitch‐hiking). Only one vicariance explanation, the eastward drift of continental fragments (shuttles) that may have contributed biota to Fiji from New Caledonia, is supported by some geological evidence, although there is no evidence for the transport of taxa on shuttle fragments. Another vicariance explanation, the existence of a major continental landmass in the Pacific within the last 100 Myr (Atlantis theory), receives little support and appears unlikely. Extinction of lineages in source areas and persistence in the TSP has probably occurred many times and has resulted in misinterpretation of biogeographical data. Main conclusions Malesia has long been considered the major source region for the biota of oceanic islands in the TSP because of shared taxa and high species diversity. However, recent molecular studies have produced compelling support for New Caledonia and Australia as alternative important source areas. They also show dispersal events, and not vicariance, to have been the major contributors to the current biota of the TSP. Past extinction events can obscure interpretations of diversity patterns. 相似文献
995.
Grazing responses in herbs in relation to herbivore selectivity and plant traits in an alpine ecosystem 总被引:1,自引:0,他引:1
Herbivores shape plant communities through selective foraging. However, both herbivore selectivity and the plant’s ability
to tolerate or resist herbivory may depend on the density of herbivores. In an alpine ecosystem with a long history of grazing,
plants are expected to respond to both enhanced and reduced grazing pressures, and the interaction between plant traits and
changes in species abundance are expected to differ between the two types of alteration of grazing regime. To understand the
mechanisms behind species response, we investigated the relationship between sheep selectivity (measured in situ), plant traits
and experimentally derived measures of change in species abundance as a response to the enhancement (from low to high density)
or cessation (from low to zero density) of sheep grazing pressure over a six-year time period for 22 abundant herb species
in an alpine habitat in south Norway. Sheep selected large, late-flowering herbs with a low leaf C/N ratio. Species that increased
in abundance in response to enhanced grazing pressure were generally small and had high root/shoot ratios, thus exhibiting
traits that reflect both resistance (through avoidance) and tolerance (through regrowth capacity) strategies. The abundance
of selected species remained stable during the study period, and also under the enhanced grazing pressure treatment. There
was, however, a tendency for selected species to respond positively to cessation of grazing, although overall responses to
cessation of grazing were much less pronounced than responses to enhanced grazing. Avoidance through short stature (probably
associated with increased light availability through the removal of tall competitors) as well as a certain amount of regrowth
capacity appear to be the main mechanisms behind a positive response to enhanced grazing pressure in this study. The plant
trait perspective clearly improves our insight into the mechanisms behind observed changes in species abundance when the disturbance
regime is altered.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
996.
Jaran S. Olsen Tone Aarskaug Gunnar Skogan Else Marie Fykse Anette Bauer Ellingsen Janet M. Blatny 《Journal of microbiological methods》2009,78(3):271-285
Vibrio cholerae is the etiological agent of cholera and may be used in bioterror actions due to the easiness of its dissemination, and the public fear for acquiring the cholera disease. A simple and highly discriminating method for connecting clinical and environmental isolates of V. cholerae is needed in microbial forensics. Twelve different loci containing variable numbers of tandem-repeats (VNTRs) were evaluated in which six loci were polymorphic. Two multiplex reactions containing PCR primers targeting these six VNTRs resulted in successful DNA amplification of 142 various environmental and clinical V. cholerae isolates. The genetic distribution inside the V. cholerae strain collection was used to evaluate the discriminating power (Simpsons Diversity Index = 0.99) of this new MLVA analysis, showing that the assay have a potential to differentiate between various strains, but also to identify those isolates which are collected from a common V. cholerae outbreak. This work has established a rapid and highly discriminating MLVA assay useful for track back analyses and/or forensic studies of V. cholerae infections. 相似文献
997.
João R. M. Almeida Magnus Bertilsson Marie F. Gorwa-Grauslund Steven Gorsich Gunnar Lidén 《Applied microbiology and biotechnology》2009,82(4):625-638
There is a growing awareness that lignocellulose will be a major raw material for production of both fuel and chemicals in
the coming decades—most likely through various fermentation routes. Considerable attention has been given to the problem of
finding efficient means of separating the major constituents in lignocellulose (i.e., lignin, hemicellulose, and cellulose)
and to efficiently hydrolyze the carbohydrate parts into sugars. In these processes, by-products will inevitably form to some
extent, and these will have to be dealt with in the ensuing microbial processes. One group of compounds in this category is
the furaldehydes. 2-Furaldehyde (furfural) and substituted 2-furaldehydes—most importantly 5-hydroxymethyl-2-furaldehyde—are
the dominant inhibitory compounds found in lignocellulosic hydrolyzates. The furaldehydes are known to have biological effects
and act as inhibitors in fermentation processes. The effects of these compounds will therefore have to be considered in the
design of biotechnological processes using lignocellulose. In this short review, we take a look at known metabolic effects,
as well as strategies to overcome problems in biotechnological applications caused by furaldehydes. 相似文献
998.
999.
Sonal Patel Elin Sørhus Ingrid Uglenes Fiksdal Per Gunnar Espedal Øivind Bergh Odd Magne Rødseth H. Craig Morton Audun Helge Nerland 《Fish & shellfish immunology》2009,26(3):385-395
In teleost fish, the head kidney, thymus, and spleen are generally regarded as important immune organs. In this study, the ontogeny of these organs was studied in Atlantic halibut (Hippoglossus hippoglossus), larvae at various stages of development. We observed that the kidney was present at hatching, the thymus at 33 days post hatch (dph), while the spleen was the last organ to be detected at 49 dph. All three lymphoid organs were morphologically well developed during late metamorphic stages. Real time RT-PCR analysis showed that IgM mRNA expression could be observed at 66 dph and later, which correlates well with in situ hybridisation data showing that a few IgM positive cells could be detected in the anterior kidney and spleen from 66 dph. Our data also showed that the highest levels of IgM mRNA could be detected in halibut spleen. Immunostaining using a monoclonal antibody against halibut IgM detected IgM positive cells at 94 dph in both the head kidney and the spleen, which is much later than the IgM mRNA. Numerous cells expressing both IgM mRNA and protein could be detected in the spleen and anterior kidney and also to some extent in thymus specimens from adult halibut. 相似文献
1000.