排序方式: 共有76条查询结果,搜索用时 31 毫秒
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Carine Chavey Gwendal Lazennec Sylviane Lagarrigue Cyrielle Clapé Irena Iankova Jacques Teyssier Jean-Sébastien Annicotte Julien Schmidt Chikage Mataki Hiroyasu Yamamoto Rosario Sanches Anna Guma Vladimir Stich Michaela Vitkova Bénédicte Jardin-Watelet Eric Renard Robert Strieter Antoinette Tuthill Gôkhan S. Hotamisligil Antonio Vidal-Puig Lluis Fajas 《Cell metabolism》2009,9(4):339-349
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Andrade CM Lopez PL Noronha BT Wink MR Borojevic R Margis R Lenz G Battastini AM Guma FC 《Cell and tissue research》2011,344(2):279-286
Ecto-5′-nucleotidase (eNT/CD73, E.C.3.1.3.5) is a glycosyl phosphatidylinositol (GPI)-linked cell-surface protein with several
functions, including the local generation of adenosine from AMP, with the consequent activation of adenosine receptors and
the salvaging of extracellular nucleotides. It also apparently functions independently of this activity, e.g., in the mediation
of cell-cell adhesion. Liver fibrosis can be considered as a dynamic and integrated cellular response to chronic liver injury
and the activation of hepatic stellate cells (HSCs) plays a role in the fibrogenic process. eNT/CD73 and adenosine are reported
to play an important role in hepatic fibrosis in murine models. Knockdown of eNT/CD73 leads to an increase in mRNA expression
of tissue non-specific alkaline phosphatase (TNALP), another AMP-degrading enzyme and thus no alteration is seen in the total
ecto-AMPase activity of the cell. eNT/CD73 knockdown also leads to changes in the expression of collagen I and a clear alteration
of cell migration. We suggest that eNT/CD73 protein expression controls cell migration and collagen expression in a mechanism
independent of changes in nucleotide metabolism. 相似文献
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Alexis C Edwards Julien F Ayroles Eric A Stone Mary Anna Carbone Richard F Lyman Trudy FC Mackay 《Genome biology》2009,10(7):R76-11
Background
Aggressive behavior is an important component of fitness in most animals. Aggressive behavior is genetically complex, with natural variation attributable to multiple segregating loci with allelic effects that are sensitive to the physical and social environment. However, we know little about the genes and genetic networks affecting natural variation in aggressive behavior. Populations of Drosophila melanogaster harbor quantitative genetic variation in aggressive behavior, providing an excellent model system for dissecting the genetic basis of naturally occurring variation in aggression. 相似文献16.
Trudy FC Mackay 《Genome biology》2000,1(4):reports4018.1-reports40186
A report on the Third Genetic Effects on Aging Meeting, The Jackson Laboratory, Bar Harbor, Maine, August 4-8, 2000. 相似文献
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Guma MK Abdeldaim Kristoffer Strålin Jens Korsgaard Jonas Blomberg Christina Welinder-Olsson Björn Herrmann 《BMC microbiology》2010,10(1):310
Background
Streptococcus pneumoniae and Haemophilus influenzae cause pneumonia and as Neisseria meningitidis they are important agents of meningitis. Although several PCR methods have been described for these bacteria the specificity is an underestimated problem. Here we present a quantitative multiplex real-time PCR (qmPCR) for detection of S. pneumoniae (9802 gene fragment), H. influenzae (omp P6 gene) and N. meningitidis (ctrA gene). The method was evaluated on bronchoalveolar lavage (BAL) samples from 156 adults with lower respiratory tract infection (LRTI) and 31 controls, and on 87 cerebrospinal fluid (CSF) samples from meningitis patients. 相似文献19.
Background
Alcoholism presents widespread social and human health problems. Alcohol sensitivity, the development of tolerance to alcohol and susceptibility to addiction vary in the population. Genetic factors that predispose to alcoholism remain largely unknown due to extensive genetic and environmental variation in human populations. Drosophila, however, allows studies on genetically identical individuals in controlled environments. Although addiction to alcohol has not been demonstrated in Drosophila, flies show responses to alcohol exposure that resemble human intoxication, including hyperactivity, loss of postural control, sedation, and exposure-dependent development of tolerance. 相似文献20.
Cardoso CC Paviani ER Cruz LA Guma FC Borojevic R Guaragna RM 《Molecular and cellular biochemistry》2003,254(1-2):37-46
In liver fibrosis, the quiescent hepatic stellate cells (HSC) are activated to proliferate and express the activated myofibroblast phenotype, losing fat droplets and the stored vitamin A, and depositing more extracellular matrix. Therapeutic strategies for liver fibrosis are focused on HSC. Pentoxifylline (PTF), an analog of the methylxanthine, prevents the biochemical and histological changes associated with animal liver fibrosis. The aim of the present study was to investigate the phenotypic change of myofibroblasts into quiescent lipocytes by PTF and/or retinol, using a permanent cell line GRX that represents murine HSC. We studied the action of both drugs on the synthesis of neutral lipids, activity of phospholipase A2 (PLA2), release of arachidonic acid (AA) and prostaglandins synthesis. Accumulation and synthesis of neutral lipids was dependent upon association of retinol with PTF. PTF (0.5 mg/mL) alone did not induce lipid accumulation and synthesis, but in cells induced by physiologic concentration of retinol (1–2.5 M), it increased the quantity of stored lipids. Retinol and PTF (5 M and 0.1 mg/mL, respectively) had a synergistic effect on neutral lipid synthesis and accumulation. In higher PTF concentrations (0.5 and 0.7 mg/ml), the synthesis was stimulated but accumulation decreased. Membrane-associated PLA2 activity decreased after PTF treatment, which increased the AA release 8 fold, and significantly increased the production of PGE2, but not of PGF2. However, when in presence of retinol, we observed a slightly higher increase in PGE2 and PGF2 production. In conclusion, PTF treatment generated an excess of free AA. We propose that retinol counteracts the action of PTF on the AA release and PGs production, even though both drugs stimulated the lipocyte induction in the HSC. 相似文献