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101.
102.
Wadhwa SK Kazi TG Chandio AA Afridi HI Kolachi NF Khan S Kandhro GA Nasreen S Shah AQ Baig JA 《Biological trace element research》2011,144(1-3):86-96
The investigated data shows that arsenic (As) in drinking water is associated with increased mortality from different types of cancers including liver cancer. In this study, blood and scalp hair samples of male liver cancer patients and healthy referents belonging to As exposed areas of Sindh Pakistan were analyzed for As contents. The As levels in drinking water of understudy area showed that sections of this population was exposed to 3-15-folds higher concentrations of As than permissible limit. For comparative purposes, blood and scalp hair samples of matched cancerous patient as referent patients belonging to big city (Hyderabad) who have used municipal treated water with low As levels <10?μg/L were also collected. The results of this study showed that the average As concentration was higher in the blood and scalp hair of exposed and non-exposed referent cancer patients as compared to referents (p?001). The exposed cancerous patients have twofolds higher As level in both biological samples as related to non-exposed case matched cancerous male subjects. 相似文献
103.
104.
Chandrabhan Seniya Harshal Mishra Ajay Yadav Nitin Sagar Babita Chaturvedi Kuldeep Uchadia Gulshan Wadhwa 《Bioinformation》2013,9(1):54-60
4-hydroxypanduratin A is a secondary metabolite of Boesenbergia pandurata Schult. (Fingerroot) plant with various pharmacological
activities such as neuroprotective, potent antioxidant, antibacterial and antifungal. Flaviviral NS2B/NS3 protease activity is
essential for polyprotein processing and viral replication for Japanese Encephalitis Virus (JEV), a major cause of Acute Encephaltis in
Asia. Inhibition of formation of this complex by arresting the binding of NS2B with NS3 would reduce the enzyme''s activity to
meager proportions and hence would prevent further viral proliferation. The automated 3D structure of NS2B protein of the JEV
GP78 was predicted based on the sequence-to-structure-to-function paradigm using I-TASSER and the function of NS2B protein
was inferred by matching to other known proteins. The stereochemical quality of predicted structure was checked by PROCHECK.
The antiviral activity of 4-hydroxypanduratin A against NS2B protein as a potential drug has been elucidated in this paper.
Docking simulation analysis showed 4-hydroxypanduratin A as potential inhibitor of NS2B protein/cofactor which is necessary for
NS3 protease activity. 220 derivatives of 4-hydroxypanduratin A were virtually screened with rigid criteria of Lipinski''s rule of 5
using Autodock4.2. 4-hydroxypanduratin A was found interacting with target hydrophilic domain in NS2B protein by two Hbonds
(Gly80 and Asp81) with active residues, several hydrophobic interactions, Log P value of 5.6, inhibition constant (Ki) of
51.07nM and lowest binding energy of -9.95Kcal/Mol. Hence, 4-hydroxypanduratin A targeted to Site 2 will have sufficient
profound effect to inhibit protease activity to abrogate viral replication. It could be a promising potential drug candidate for JEV
infections using NS2B Site 2 as a Drug target. 相似文献
105.
Sumbul Firdaus Anupam Dhasmana Vandana Srivastava Tasneem Bano Afreen Fatima Qazi Mohammad Sajid Jamal Roshan Jahan Gulshan Wadhwad Mohtashim Lohani 《Bioinformation》2015,11(3):122-126
Fullerenes have attracted considerable attention due to their unique chemical structure and potential applications which has
opened wide venues for possible human exposure to various fullerene types. Therefore, in depth knowledge of how fullerene may
interfere with various cellular processes becomes quite imperative. The present study was designed to investigate how the
presence of fullerene affect the binding of DNA with different enzymes involved in replication process. Different fullerenes were
first docked with DNA and then binding scores of different enzymes was analyzed with fullerene docked DNA. C30, C40 & C50
once docked with DNA, reduced the binding score of primase, whereas no significant change in the binding score was observed
with the helicase, ssb protein, dna pol δ, dna pol ε, ligase, DNA clamp, and topoisomerases. On the contrast, the binding score of
RPA14 decreases in fluctuating manner while interacting with increasing molecular weight of fullerene bound single-stranded
DNA complex. The study revealed the affect of fullerene family interacting with DNA on the binding pattern of enzymes involved
in replication process. Study suggests that the presence of most of fullerenes may not affect the activity of these enzymes necessary
for replication process whereas C30, C40 & C50 may disrupt the activity of primase, (strating point for DNA polymerase) its
docking score decreases from 13820 to 10702. 相似文献
106.
107.
Neha Wadhwa Udai Narayan Joshi Sudarshan Kumar Gandhi 《Journal of Phytopathology》2013,161(3):172-179
Clusterbean seedlings growing in soil inoculated with Rhizoctonia were treated with either 5 or 10 ppm Cu2+ and Mn2+. Soil was inoculated by pretreatment with 250 mg (wet weight) of Rhizoctonia inoculum. A similar set of plants was maintained in uninoculated soil. Root rot incidence of plants treated with Cu2+ 5 ppm, Cu2+ 10 ppm, Mn2+ 5 ppm and Mn2+ 10 ppm was 26.6, 30.5, 11.8 and 29.2% less than the inoculated control, respectively. Inoculation with Rhizoctonia reduced chlorophyll, non‐structural carbohydrate and in vitro dry matter digestibility (IVDMD) content compared with uninoculated ones. Oxidative enzymes activities (polyphenol oxidase, peroxidase, phenylalanine ammonia lyase and tyrosine ammonia lyase), crude protein, phenolic content, structural components (acid detergent fibre, cellulose and lignin), silica, macronutrients and micronutrients increased in inoculated seedlings and this increase was further heightened by the Cu2+ 10 ppm treatment compared with the Cu2+ 5 ppm, Mn2+ 5 ppm and Mn2+ 10 ppm treatments in response to fungal invasion. It was concluded that the Cu2+ 10 ppm treatment may be an effective soil nutrient to provide enhanced resistance of clusterbean plants to root rot (fungal) diseases. 相似文献
108.
Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres 总被引:4,自引:0,他引:4
Zhong ZH Jiang WQ Cesare AJ Neumann AA Wadhwa R Reddel RR 《The Journal of biological chemistry》2007,282(40):29314-29322
Immortalized human cells are able to maintain their telomeres by telomerase or by a recombination-mediated DNA replication mechanism known as alternative lengthening of telomeres (ALT). We showed previously that overexpression of Sp100 protein can suppress ALT and that this was associated with sequestration of the MRE11/RAD50/NBS1 (MRN) recombination protein complex by Sp100. In the present study, we determined whether MRN proteins are required for ALT activity. ALT cells were depleted of MRN proteins by small hairpin RNA-mediated knockdown, which was maintained for up to 100 population doublings. Knockdown of NBS1 had no effect on the level of RAD50 or MRE11, but knockdown of RAD50 also depleted cells of NBS1, and knockdown of MRE11 depleted cells of all three MRN proteins. Depletion of NBS1, with or without depletion of other members of the complex, resulted in inhibition of ALT-mediated telomere maintenance, as evidenced by decreased numbers of ALT-associated promyelocytic leukemia bodies and decreased telomere length. In some clones there was an initial period of rapid shortening followed by stabilization of telomere length, whereas in others there was continuous shortening at a rate within the reported range for normal human somatic cells lacking a telomere maintenance mechanism. In contrast, depletion of NBS1 in telomerase-positive cells did not result in telomere shortening. A recent study showed that NBS1 was required for the formation of extrachromosomal telomeric circles (Compton, S. A., Choi, J. H., Cesare, A. J., Ozgur, S., and Griffith, J. D. (2007) Cancer Res. 67, 1513-1519), also a marker for ALT. We conclude that the MRN complex, and especially NBS1, is required for the ALT mechanism. 相似文献
109.
110.
Saqib Bashir Muzammal Rehman Muhammad Yousaf Abdus Salam Allah Bakhsh Gulshan Javaid Iqbal 《International journal of phytoremediation》2019,21(11):1098-1103
AbstractBiochar is considered a novel soil amendment for cadmium (Cd) stabilization in contaminated soils. A pot experiment was conducted to examine the efficiency of wheat straw and sugarcane bagasse induced biochar on Cd mobility in soil and its bioavailability to spinach in contaminated soil. Soil pH, Cd contents in plant tissues and microbial biomass were examined. Results showed that Cd was significantly decreased by 30.95% and 20.83% with wheat straw and sugarcane bagasse biochar at 2% application rate respectively, relative to the control. Similarly, Cd contents were decreased in plants shoots by 15.41 and 14.33%, while in roots by 48.3 and 35.54%, when wheat straw and sugarcane biochar were added at 2% application rate respectively. Moreover, soil microbial biomass was significantly increased with the application of all biochar types and their applications rates. Finally, wheat straw biochar at 2% application rate can be considered as an effective approach for Cd stabilization in contaminated soils. 相似文献