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71.
Involvement of purinergic 2X4 receptor in glycoprotein 120‐induced pyroptosis in dorsal root ganglia
72.
Yan Wang Qing-Chuan Zheng Ji-Long Zhang Ying-Lu Cui Qiao Xue Hong-Xing Zhang 《Journal of molecular modeling》2013,19(12):5213-5223
Cytosolic insect theta class glutathione S-transferases (GSTs) have not been studied completely and their physiological roles are unknown. A detailed understanding of Anopheles gambiae GST (Aggst1-2) requires an accurate structure, which has not yet been determined. A high quality model structure of Aggst1-2 was constructed using homology modeling and the ligand–protein complex was obtained by the docking method. Molecular dynamics (MD) simulations were carried out to study conformational changes and to calculate binding free energy. The results of MD simulation indicate that Aggst1-2 undergoes small conformational changes after ligands dock to the protein, which facilitate the catalytic reaction. An essential hydrogen bond was found between the sulfur atom of glutathione (GSH) and the hydrogen atom of hydroxyl group in Ser9, which was in good agreement with experimental data. A π–π interaction between Phe204 and CDNB ligand was also found. This interaction seems to be important in stabilization of the ligand. Further study of binding free energy decomposition revealed a van der Waals interaction between two ligands that may play a key role in nucleophilic addition reaction. This work will be a good starting point for further determination of the biological role of cytosolic insect theta class GSTs and will aid the design of structure-based inhibitors. 相似文献
73.
Jinrui Gan Kun Qian Jingjing Wan Liang Qiao Weichao Guo Pengyuan Yang Hubert H. Girault Baohong Liu 《Proteomics》2013,13(21):3117-3123
Amino‐functionalized macroporous silica foam (NH2‐MOSF) has been developed as a host reactor to realize highly efficient proteolysis in acidic solutions where normal tryptic reactions cannot occur. The digestion protocol consists simply of adding the functionalized NH2‐MOSF into the protein and trypsin solutions without altering the bulk pH or preloading the enzymes on the materials. With this protocol, digestion of sample fractions from LC can be efficiently realized in the acidic solutions directly. Digestion of a protein fraction extracted from rat liver tissue after LC separation was performed to illustrate this principle, where 103 proteins were successfully identified at pH 3 after 1.5 h of tryptic digestion. 相似文献
74.
Peiyuan He Yuejin Yang Shubin Qiao Bo Xu Min Yao Yongjian Wu Jinqing Yuan Jue Chen Haibo Liu Jun Dai Xiao Yang Xinran Tang Yang Wang Wei Li Runlin Gao 《PloS one》2015,10(3)
Background
Transradial approach (TRA) outweighed transfemoral approach (TFA) in acute coronary syndrome patients because the former has better short-term outcomes in high-volume percutaneous coronary intervention (PCI) centers. Our study was one of the limited studies specifically in comparing the short- and medium-term effects of TRA and those of TFA in patients undergoing elective PCIs.Methods
A total of 21,242 patients who underwent elective PCI with stent implantation were included. Using propensity score methodology, 1,634 patient pairs were matched. Major clinical outcomes and PCI-related complications between TRA and TFA were compared.Results
In the propensity score-matched patients, the rates of in-hospital net adverse clinical events, which included death, myocardial infarction (MI), target vessel revascularization (TVR), stroke, and major bleeding, were much lower with TRA than with TFA (1.8% vs. 3.9%, P < 0.001). This difference was mainly due to the lower rate of major bleeding (0.6% vs. 1.8%, P < 0.001) and the decreased rate of MI (1.1% vs. 1.9%, P = 0.060). PCI-related dissection and thrombosis were similar between the TRA and TFA groups (both P > 0.05). Meanwhile, one-year incidence rates of major adverse cardiovascular events, which included death, MI, and TVR, were also similar (4.1% vs. 4.9%, P = 0.272) in TRA and TFA. Multivariable regression analyses showed that TRA was an independent predictor of the low rate of in-hospital net adverse clinical events (odds ratio, 0.53; 95% confidence interval, 0.40 to 0.71), but not of major adverse cardiovascular events at one-year follow-up (hazard ratio, 1.01; 95% confidence interval, 0.96 to 1.06).Conclusions
In patients undergoing elective PCI, TRA patients had lower rates of in-hospital net adverse clinical outcomes compared with TFA patients. TRA might be recommended as a routine approach in high-volume PCI hospitals for elective PCIs. 相似文献75.
Spawning activity of the four major Chinese carps in the middle mainstream of the Yangtze River,during the Three Gorges Reservoir operation period,China 下载免费PDF全文
W. Xu Y. Qiao X.‐J. Chen Y.‐P. Cai Z. Yang H.‐G. Liu 《Zeitschrift fur angewandte Ichthyologie》2015,31(5):846-854
River flow alterations caused by dams have introduced many ecological problems, in particular a decline in aquatic species such as fishes. One compensatory measure is to create a hydrological process similar to the natural state with regard to the survival requirements of the fish. In recent years, the Three Gorges Reservoir (TGR) has introduced man‐made flood by ecological operation experiments to facilitate spawning of the four major Chinese carps in the Yangtze River, China. To investigate the fish spawning activities and their responses to the TGR operation, eggs from the four major Chinese carps were sampled using conical drift nets in the middle mainstream of the Yangtze River, May to July in 2012 and 2013. Spawning timing, location, and scale of the four carps were studied and compared between the 2 years; key hydrological and environmental factors associated with spawning were determined by principal component analysis and stepwise regression analysis. Two factors were significantly positive when correlated with egg abundance: one was increasing rate of the river flow (flood amplitude), and the other was river transparency; only one factor, starting of the river flow (flooding occasions), was significantly and negatively correlated with the time of spawning. Comparison of egg abundance in one flood pulse response to different operation rules showed that flooding made by an ecological operation induced a larger scale of spawning than a conventional operation. The study implied that suitable flood conditions could produce a successful spawning event, and that the occasion and pattern of the flood process might result in different responses in fish spawning. Further research is required to develop more scientific monitoring designs in order to obtain accurate field data for both biotic and abiotic factors, and explore new research methods for egg abundance estimations combined with particle experiment and hydrodynamic modeling. This work is fundamental to improve the strategic decisions on reservoir operation and river management. 相似文献
76.
77.
Diachronic analysis of genetic diversity in rice landraces under on-farm conservation in Yunnan,China 总被引:1,自引:0,他引:1
78.
Dai Chunxiao Ma Qiao Li Yan Zhou Duandi Yang Bingyu Qu Yuanyuan 《Bioprocess and biosystems engineering》2019,42(12):1963-1971
Bioprocess and Biosystems Engineering - Indigo, one of the most widely used dyes, is mainly produced by chemical processes, which generate amounts of pollutants and need high energy consumption.... 相似文献
79.
Qiao Huang Kostiantyn Turcheniuk Xiaolei Ren Alexandre Magasinski Daniel Gordon Nasr Bensalah Gleb Yushin 《Liver Transplantation》2019,9(17)
As an alternative to commercial Ni‐ and Co‐based intercalation‐type cathode materials, conversion‐type metal fluoride (MFx) cathodes are attracting more interest due to their promises to increase cell‐level energy density when coupled with lithium (Li) or silicon (Si)‐based anodes. Among metal fluorides, iron fluorides (FeF2 and FeF3) are regarded as some of the most promising candidates due to their high capacity, moderately high potential and the very low cost of Fe. In this study, the impacts of electrolyte composition on the performance and stability of nanostructured FeF2 cathodes are systematically investigated. Dramatic impacts of Li salt composition, Li salt concentration, solvent composition, and cycling potential range on the cathode's most critical performance parameters—stability, capacity, rate, and voltage hysteresis are discovered. In contrast to previous beliefs, it is observed that even if the Fe2+ cation dissolution could be avoided, the dissolution of F? anions may still negatively affect cathode performance. Formation of the more favorable cathode solid electrolyte interface (CEI) is found to minimize both processes. 相似文献
80.
Ya Zheng Chen Dong Junling Yang Yi Jin Wenjie Zheng Qiao Zhou Yi Liang Liuliu Bao Guijuan Feng Juan Ji Xingmei Feng Zhifeng Gu 《Journal of cellular physiology》2019,234(11):20662-20674
The mechanism of local inflammation and systemic injury in chronic periodontitis is complicated, in which and exosomes play an important role. In our study, we found that T helper cell 17 (Th17)/regulatory T cell (Treg) balance is destabilized in the peripheral blood of patients with periodontitis, with upregulated Th17 or downregulated Treg, respectively. Porphyromonas gingivalis lipopolysaccharide (LPS) was used to simulate the inflammatory microenvironment of chronic periodontitis. The exosomes were extracted from periodontal ligament stem cells (PDLSCs) in LPS-induced periodontitis environment, which inversely effected on CD4+ T cells under normal and inflammatory conditions. Furthermore, compared with exosomes from normal PDLSCs, lower expression of microRNA-155-5p (miR-155-5p) and higher expression of Sirtuin-1 (SIRT1) were observed in exosomes from LPS-stimulated PDLSCs. Exosomes from PDLSCs alleviated inflammatory microenvironment through Th17/Treg/miR-155-5p/SIRT1 regulatory network. This study aimed to find the “switching” factors that affected the further deterioration of periodontitis to maximally control the multiple downstream damage signal factors to further understand periodontitis and find new targets for its treatment. 相似文献