全文获取类型
收费全文 | 72212篇 |
免费 | 5980篇 |
国内免费 | 61篇 |
出版年
2023年 | 365篇 |
2022年 | 731篇 |
2021年 | 1483篇 |
2020年 | 1073篇 |
2019年 | 1211篇 |
2018年 | 1747篇 |
2017年 | 1431篇 |
2016年 | 2256篇 |
2015年 | 3144篇 |
2014年 | 3216篇 |
2013年 | 3995篇 |
2012年 | 4753篇 |
2011年 | 4385篇 |
2010年 | 2734篇 |
2009年 | 2599篇 |
2008年 | 3156篇 |
2007年 | 3184篇 |
2006年 | 2889篇 |
2005年 | 3033篇 |
2004年 | 2995篇 |
2003年 | 2535篇 |
2002年 | 2136篇 |
2001年 | 1837篇 |
2000年 | 1778篇 |
1999年 | 1564篇 |
1998年 | 740篇 |
1997年 | 694篇 |
1996年 | 712篇 |
1995年 | 599篇 |
1994年 | 576篇 |
1993年 | 539篇 |
1992年 | 1095篇 |
1991年 | 976篇 |
1990年 | 883篇 |
1989年 | 900篇 |
1988年 | 864篇 |
1987年 | 785篇 |
1986年 | 723篇 |
1985年 | 708篇 |
1984年 | 655篇 |
1983年 | 514篇 |
1982年 | 403篇 |
1981年 | 390篇 |
1980年 | 350篇 |
1979年 | 491篇 |
1978年 | 417篇 |
1977年 | 345篇 |
1975年 | 344篇 |
1974年 | 367篇 |
1973年 | 351篇 |
排序方式: 共有10000条查询结果,搜索用时 671 毫秒
91.
Jennifer Beckensteiner David M. Kaplan Warren M. Potts Carmen V. Santos Michael R. O’Farrell 《PloS one》2016,11(2)
Excessive truncation of a population’s size structure is often identified as an important deleterious effect of exploitation, yet the effect on population persistence of size-structure truncation caused by exploitation is often not quantified due to data limitations. In this study, we estimate changes in eggs per recruit (EPR) using annual length-frequency samples over a 9 year period to assess persistence of the two most important recreational fishes in southern Angola: west coast dusky kob (Argyrosomus coronus) and leerfish (Lichia amia). Using a length- and age-structured model, we improve on an existing method to fit this type of model to length-frequency data and estimate EPR. The objectives of the methodological changes are to add flexibility and robustness to the approach for assessing population status in data-limited situations. Results indicate that dusky kob presents very low levels of EPR (5%-10% of the per recruit reproductive capacity in the absence of fishing) in 2013, whereas large inter-annual variability in leerfish estimates suggest caution must be applied when drawing conclusions about its exploitation status. Using simulated length frequency data with known parameter values, we demonstrate that recruitment decline due to overexploitation leads to overestimation of EPR values. Considering the low levels of EPR estimated for the study species, recruitment limitation is not impossible and true EPR values may be even lower than our estimates. It is, therefore, likely that management action, such as the creation of Marine Protected Areas, is needed to reconstitute the west coast dusky kob population. 相似文献
92.
Martin T. R. Kuiper Marijke Holtrop Hans de Vries 《Molecular & general genetics : MGG》1988,213(2-3):519-528
93.
94.
95.
96.
I. Albesa P. Bogdanov A. Eraso N.R. Sperandeo M.M. de Bertorello 《Journal of applied microbiology》1995,78(4):373-377
I. ALBESA, P. BOGDANOV, A. ERASO, N.R. SPERANDEO AND M.M. DE BERTORELLO. 1995. The antibiotic activity of new synthetic isoxazolylnaphthoquinone imines was studied. Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922 were resistant to the four compounds studied (MIC > 128 µg ml−1 ), but Staphylococcus aureus ATCC 25923, ATCC 29213 and 30 clinical isolates of Staph. aureus were inhibited by 2-hydroxy- N -(3,4-dimethyl-5-isoxazolyl)-1,4-naphthoquinone-4-imine (I). This compound diminished bloodstream infection of mice injected i.m. with Staph. aureus; septicaemia decayed significantly when I was applied at the beginning of the infection while when I was given 3 d after bacterial challenge, a significant protection was afforded. Bactericidal activity in serum increased during the 5 h after I was administered i.p.
The acetyl derivative of I had a high MIC but when inoculated orally in mice decreased the Staph. aureus counts in circulation. This protection occurred only when the schedule of administration started close to the bacterial challenge. Antibiotic activity in vivo may be associated with in vitro effects. 相似文献
The acetyl derivative of I had a high MIC but when inoculated orally in mice decreased the Staph. aureus counts in circulation. This protection occurred only when the schedule of administration started close to the bacterial challenge. Antibiotic activity in vivo may be associated with in vitro effects. 相似文献
97.
Cassandre Leverrier Giana Almeida Lucia Espinosa-Mu noz Gérard Cuvelier 《Food biophysics》2016,11(3):235-247
This work investigates the impact of structural parameters on the rheological behaviour of apple purees. Reconstructed apple purees from 0 g/100 g up to 2.32 g/100 g of insoluble solids content and varying in particle size were prepared. Three different particle size distributions were obtained by mechanical treatment only, to modify both size and morphology of the particles without modifying the intrinsic rigidity of the cell walls. Rheological measurements showed that the insoluble solids content have a first order effect on the rheological behaviour of the suspensions: three concentrations domains were observed in both dynamic and flow measurements. A model is proposed for each domain. The existence of a weak network between particles is clearly shown over a critical concentration of insoluble solids (cell walls) depending on particle size distribution (semi-diluted domain). In a concentrated domain, particles are on close packing conditions and their apparent volume begin to shrink. Particle size and shape also play an important role on the rheological behaviour of reconstructed apple puree. Due to their irregular shape, cell clusters clog the medium at lower concentration compared to individual cells. 相似文献
98.
We have studied the immunomodulatory properties of epithelial cells from the small intestine on T cell immune function in vitro. Proliferation of lymph node cells stimulated either with antigen or with mitogen was inhibited by epithelial cells in a dose-dependent fashion. The epithelial cell-mediated suppression of lymphocyte proliferation was blocked by indomethacin, a cyclooxygenase pathway inhibitor, demonstrating that the suppressive effect of epithelial cells was related to prostaglandin secretion. Furthermore, the action of epithelial cell-secreted prostaglandin on lymphocytes was related to its effect on IL-2 as the suppressive effect of epithelial cells was abrogated by the addition of exogenous IL-2. As previously reported, epithelial cells constitutively express MHC class II and we found them able to present antigen in a class II-restricted fashion when their suppressive effects were blocked by indomethacin. Furthermore, epithelial cells activated by LPS secrete an IL-1 like molecule in a fashion analogous to other antigen-presenting cells. These results demonstrate that epithelial cells can both enhance and suppress in vitro T cell immune responses and further characterize the mechanisms by which intestinal epithelial cells may function in gut-associated immune responses. 相似文献
99.
100.