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221.
Medicago truncatula is a legume species belonging to the inverted repeat lacking clade (IRLC) with trifoliolate compound leaves. However, the regulatory mechanisms underlying development of trifoliolate leaves in legumes remain largely unknown. Here, we report isolation and characterization of fused compound leaf1 (fcl1) mutants of M. truncatula. Phenotypic analysis suggests that FCL1 plays a positive role in boundary separation and proximal-distal axis development of compound leaves. Map-based cloning indicates that FCL1 encodes a class M KNOX protein that harbors the MEINOX domain but lacks the homeodomain. Yeast two-hybrid assays show that FCL1 interacts with a subset of Arabidopsis thaliana BEL1-like proteins with slightly different substrate specificities from the Arabidopsis homolog KNATM-B. Double mutant analyses with M. truncatula single leaflet1 (sgl1) and palmate-like pentafoliata1 (palm1) leaf mutants show that fcl1 is epistatic to palm1 and sgl1 is epistatic to fcl1 in terms of leaf complexity and that SGL1 and FCL1 act additively and are required for petiole development. Previous studies have shown that the canonical KNOX proteins are not involved in compound leaf development in IRLC legumes. The identification of FCL1 supports the role of a truncated KNOX protein in compound leaf development in M. truncatula. 相似文献
222.
We have developed a simple approach for generating peptide-conjugated gold nanoparticles (AuNPs) from the Rev peptide and gold aqueous solution. The peptide functions as both a reducing agent and a capping molecule. AuNPs of various sizes (20-300 nm) and shapes (spheres, triangular plates, and polygons) can be obtained upon modulating the ratio of gold ions to the Rev peptide. Transmission electron microscopy, X-ray diffraction, and UV-vis spectroscopy were utilized to characterize these nanoparticles. Fourier-transform infrared and X-ray photoelectron spectroscopy measurements were performed to investigate chemical interactions between the Rev peptide and AuNPs. Lactate dehydrogenase and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays revealed that the Rev peptide-AuNP nanocomposites exhibited exceptionally high cytotoxic effects toward mouse ovarian surface epithelial cell lines, relative to the effects of equal doses of the free Rev peptide. Our study suggests a new way of utilizing biomolecule-conjugated AuNPs as potentially effective anticancer drugs. 相似文献
223.
Xuan J Pan G Qiu Y Yang L Su M Liu Y Chen J Feng G Fang Y Jia W Xing Q He L 《Journal of proteome research》2011,10(12):5433-5443
Despite recent advances in understanding the pathophysiology of schizophrenia and the mechanisms of antipsychotic drug action, the development of biomarkers for diagnosis and therapeutic monitoring in schizophrenia remains challenging. Metabolomics provides a powerful approach to discover diagnostic and therapeutic biomarkers by analyzing global changes in an individual's metabolic profile in response to pathophysiological stimuli or drug intervention. In this study, we performed gas chromatography-mass spectrometry based metabolomic profiling in serum of unmedicated schizophrenic patients before and after an 8-week risperidone monotherapy, to detect potential biomarkers associated with schizophrenia and risperidone treatment. Twenty-two marker metabolites contributing to the complete separation of schizophrenic patients from matched healthy controls were identified, with citrate, palmitic acid, myo-inositol, and allantoin exhibiting the best combined classification performance. Twenty marker metabolites contributing to the complete separation between posttreatment and pretreatment patients were identified, with myo-inositol, uric acid, and tryptophan showing the maximum combined classification performance. Metabolic pathways including energy metabolism, antioxidant defense systems, neurotransmitter metabolism, fatty acid biosynthesis, and phospholipid metabolism were found to be disturbed in schizophrenic patients and partially normalized following risperidone therapy. Further study of these metabolites may facilitate the development of noninvasive biomarkers and more efficient therapeutic strategies for schizophrenia. 相似文献
224.
To investigate the expression and biological significance of Leptin, Leptin receptor, Vascular Endothelial Growth Factor (VEGF),
and CD34 protein in colorectal carcinoma tissues. The expression of Leptin, Leptin receptor, VEGF, and CD34 was detected in
68 cases of colorectal carcinoma tissues, paired para-carcinoma tissues and normal colorectal tissues by Immunohistochemical
SP Method. The results and related clinicopathological data were analyzed. The positive rate of Leptin, Leptin receptor, and
VEGF was significantly higher in colorectal carcinoma tissues than that in paired para-carcinoma tissues and normal colorectal
tissues. The expression of Leptin, Leptin receptor, and VEGF was correlated with grade of tumor differentiation, depth of
bowel wall invasion, lymph node metastasis, Dukes stage, distant metastasis, and lympho/vascular tumor embolization. Microvessel
density (MVD) value in colorectal carcinoma was significantly higher than that in para-carcinoma tissues and normal colorectal
tissues, and the density in para-carcinoma tissues was higher than that in normal colorectal tissues. The expression of Leptin,
Leptin receptor, VEGF, and MVD value in colorectal carcinoma was positively correlated. In conclusion, microvessel density
value is an important index of the growth, invasion, and metastasis of colorectal carcinoma. The binding of Leptin and Leptin
receptor promotes the proliferation of colorectal carcinoma cells. The synergy between Leptin and VEGF accelerates the angiogenesis
in colorectal carcinoma and accelerates the invasion and metastasis of the tumor cells. 相似文献
225.
Tran TT Brown LE Coburn JW Lynn SK Dabbs NC Schick MK Schick EE Khamoui AV Uribe BP Noffal GJ 《Journal of strength and conditioning research / National Strength & Conditioning Association》2011,25(12):3472-3478
Tran, TT, Brown, LE, Coburn, JW, Lynn, SK, Dabbs, NC, Schick, MK, Schick, EE, Khamoui, AV, Uribe, BP, and Noffal, GJ. Effects of different elastic cord assistance levels on vertical jump. J Strength Cond Res 25(12): 3472-3478, 2011-Currently, little research has been conducted using body weight reduction (BWR) as a means to enhance vertical jump. The purpose of this study was to determine the effects of different elastic cord assistance levels on vertical jump height (JH), takeoff velocity (TOV), relative ground reaction force (rGRF), relative impact force (RIF), and descent velocity (DV). Thirty recreationally trained college men and women (M = 15, W = 15) completed 3 testing sessions consisting of 5 conditions: 0, 10, 20, 30, and 40% BWR. In all BWR conditions, the subjects wore a full body harness while being attached to 2 elastic cords suspended from the ceiling and a linear velocity transducer. They then performed 3 maximal countermovement jumps with arm swing on a force plate. The results indicated no interaction of condition by sex for any variable; however, there was a significant (p < 0.05) main effect for condition for each variable. The JH significantly increased across all conditions (0%: 43.73 ± 1.62 cm, 40%: 64.77 ± 2.36 cm). The TOV at 30% (2.73 ± 0.34 m·s) was significantly greater than that at 0% (2.59 ± 0.39 m·s) and 10% (2.63 ± 0.34 m·s), whereas that at 40% (2.79 ± 0.43 m·s) was significantly greater than that at >0, 10, and 20%. The rGRF at 30% (18.62 ± 4.35 N·kg) was significantly greater than that at >0, 10, and 20%, whereas that at 40% (21.38 ± 5.21 N·kg) was significantly greater than in all conditions. The RIF at 20, 30, and 40% (40%: 61.60 ± 18.53 N·kg) was significantly greater than that at 0% (44.46 ± 9.12 N·kg). The DV at 20% (2.61 ± 0.31 m·s) was significantly greater than at 10%, whereas those at 30 and 40% (2.8 ± 0.41 m·s) were significantly greater than at 0, 10, and 20%. These results demonstrate that using different elastic cord levels to reduce body weight appears effective for increasing ascent and descent force and velocity variables. Future research should investigate greater BWR% and chronic training. 相似文献
226.
Background
Pollen development in flowering plants requires strict control of the gene expression program and genetic information stability by mechanisms possibly including the miRNA pathway. However, our understanding of the miRNA pathway in pollen development remains limited, and the dynamic profile of miRNAs in developing pollen is unknown. 相似文献227.
Background
Colorectal cancer (CRC) is one of the most common malignancies but the current therapeutic approaches for advanced CRC are less efficient. Thus, novel therapeutic approaches are badly needed. The purpose of this study is to investigate the involvement of nuclear protein kinase CK2 α subunit (CK2α) in tumor progression, and in the prognosis of human CRC.Methodology/Principal Findings
Expression levels of nuclear CK2α were analyzed in 245 colorectal tissues from patients with CRC by immunohistochemistry, quantitative real-time PCR and Western blot. We correlated the expression levels with clinicopathologic parameters and prognosis in human CRC patients. Overexpression of nuclear CK2α was significantly correlated with depth of invasion, nodal status, American Joint Committee on Cancer (AJCC) staging, degree of differentiation, and perineural invasion. Patients with high expression levels of nuclear CK2α had a significantly poorer overall survival rate compared with patients with low expression levels of nuclear CK2α. In multi-variate Cox regression analysis, overexpression of nuclear CK2α was proven to be an independent prognostic marker for CRC. In addition, DLD-1 human colon cancer cells were employed as a cellular model to study the role of CK2α on cell growth, and the expression of CK2α in DLD-1 cells was inhibited by using siRNA technology. The data indicated that CK2α-specific siRNA treatment resulted in growth inhibition.Conclusions/Significance
Taken together, overexpression of nuclear CK2α can be a useful marker for predicting the outcome of patients with CRC. 相似文献228.
229.
Background
MPAs (minor physical anomalies) frequently occur in neurodevelopmental disorders because both face and brain are derived from neuroectoderm in the first trimester. Conventionally, MPAs are measured by evaluation of external appearance. Using MRI can help overcome inherent observer bias, facilitate multi-centre data acquisition, and explore how MPAs relate to brain dysmorphology in the same individual. Optical MPAs exhibit a tightly synchronized trajectory through fetal, postnatal and adult life. As head size enlarges with age, inter-orbital distance increases, and is mostly completed before age 3 years. We hypothesized that optical MPAs might afford a retrospective ‘window’ to early neurodevelopment; specifically, inter-orbital distance increase may represent a biomarker for early brain dysmaturation in autism.Methods
We recruited 91 children aged 7–16; 36 with an autism spectrum disorder and 55 age- and gender-matched typically developing controls. All children had normal IQ. Inter-orbital distance was measured on T1-weighted MRI scans. This value was entered into a voxel-by-voxel linear regression analysis with grey matter segmented from a bimodal MRI data-set. Age and total brain tissue volume were entered as covariates.Results
Intra-class coefficient for measurement of the inter-orbital distance was 0.95. Inter-orbital distance was significantly increased in the autism group (p = 0.03, 2-tailed). The autism group showed a significant relationship between inter-orbital distance grey matter volume of bilateral amygdalae extending to the unci and inferior temporal poles.Conclusions
Greater inter-orbital distance in the autism group compared with healthy controls is consistent with infant head size expansion in autism. Inter-orbital distance positively correlated with volume of medial temporal lobe structures, suggesting a link to “social brain” dysmorphology in the autism group. We suggest these data support the role of optical MPAs as a “fossil record” of early aberrant neurodevelopment, and potential biomarker for brain dysmaturation in autism. 相似文献230.