全文获取类型
收费全文 | 945篇 |
免费 | 112篇 |
专业分类
1057篇 |
出版年
2023年 | 4篇 |
2022年 | 9篇 |
2021年 | 20篇 |
2020年 | 10篇 |
2019年 | 8篇 |
2018年 | 31篇 |
2017年 | 23篇 |
2016年 | 30篇 |
2015年 | 35篇 |
2014年 | 36篇 |
2013年 | 55篇 |
2012年 | 47篇 |
2011年 | 59篇 |
2010年 | 42篇 |
2009年 | 33篇 |
2008年 | 22篇 |
2007年 | 34篇 |
2006年 | 22篇 |
2005年 | 31篇 |
2004年 | 35篇 |
2003年 | 30篇 |
2002年 | 28篇 |
2001年 | 28篇 |
2000年 | 29篇 |
1999年 | 25篇 |
1998年 | 24篇 |
1997年 | 11篇 |
1996年 | 8篇 |
1995年 | 15篇 |
1994年 | 13篇 |
1993年 | 14篇 |
1992年 | 19篇 |
1991年 | 16篇 |
1990年 | 17篇 |
1989年 | 15篇 |
1988年 | 21篇 |
1987年 | 19篇 |
1986年 | 26篇 |
1985年 | 15篇 |
1984年 | 10篇 |
1983年 | 14篇 |
1982年 | 9篇 |
1981年 | 7篇 |
1980年 | 5篇 |
1978年 | 7篇 |
1977年 | 8篇 |
1975年 | 7篇 |
1973年 | 6篇 |
1971年 | 4篇 |
1969年 | 4篇 |
排序方式: 共有1057条查询结果,搜索用时 0 毫秒
61.
Mauricio Toro‐Nahuelpan Laura Corrales‐Guerrero Theresa Zwiener Manuel Osorio‐Valeriano Frank‐Dietrich Müller Jürgen M. Plitzko Marc Bramkamp Martin Thanbichler Dirk Schüler 《Molecular microbiology》2019,112(5):1423-1439
Cell division needs to be tightly regulated and closely coordinated with other cellular processes to ensure the generation of fully viable offspring. Here, we investigate division site placement by the cell division regulator MipZ in the alphaproteobacterium Magnetospirillum gryphiswaldense, a species that forms linear chains of magnetosomes to navigate within the geomagnetic field. We show that M. gryphiswaldense contains two MipZ homologs, termed MipZ1 and MipZ2. MipZ2 localizes to the division site, but its absence does not cause any obvious phenotype. MipZ1, by contrast, forms a dynamic bipolar gradient, and its deletion or overproduction cause cell filamentation, suggesting an important role in cell division. The monomeric form of MipZ1 interacts with the chromosome partitioning protein ParB, whereas its ATP‐dependent dimeric form shows non‐specific DNA‐binding activity. Notably, both the dimeric and, to a lesser extent, the monomeric form inhibit FtsZ polymerization in vitro. MipZ1 thus represents a canonical gradient‐forming MipZ homolog that critically contributes to the spatiotemporal control of FtsZ ring formation. Collectively, our findings add to the view that the regulatory role of MipZ proteins in cell division is conserved among many alphaproteobacteria. However, their number and biochemical properties may have adapted to the specific needs of the host organism. 相似文献
62.
Jeimy D. Santiago‐Valentín Alma P. Rodríguez‐Troncoso Eric Bautista‐Guerrero Andrs Lpez‐Prez Amilcar L. Cupul‐Magaa 《Invertebrate Biology》2019,138(1):29-39
Porites panamensis is a hermatypic brooder coral endemic to, and distributed along, the Eastern Tropical Pacific, and is considered a species vulnerable to local effects because it has limited capacity for long‐distance dispersal (and low genetic diversity). Although larvae of P. panamensis have been previously shown to recruit to artificial settlement platforms, they have never been observed in the water column. The present study describes the reproductive behavior of P. panamensis, with a focus on using molecular tools to document evidence for a larval planktonic stage and for successful recruitment. Larvae collected from the water column, and recruitment on natural and artificial substrata were documented. Phylogenetic analysis of two ribosomal markers, 18s rRNA and ITS (ITS1‐5.8‐ITS2), and one mitochondrial marker, cytochrome oxidase subunit 1 (cox1), confirmed the taxonomic identity of larvae, and showed that larvae and recruits have genotypes similar to adults of P. panamensis. Lipid vacuoles and Symbiodinium sp. were present in the gastrodermis of all larvae. A total of 12 and 371 recruits settled on artificial and natural substrates, respectively, and the recruitment rate differed significantly over time. By documenting the reproductive success of the species, we show the potential for existing individuals both to maintain the population in the study area and to contribute to maintenance of the coral reef community in the coming decades. 相似文献
63.
Melatonin could act on immune system by regulating cytokine production of immunocompetent cells. The hormone enhances IL-2, IFN-gamma and IL-6 production by cultured human mononuclear cells. As enhancement of IL-6 production is related to monocyte activation by melatonin, the hormone acts on human lymphoid cells causing a Th1-type response. This paper shows that melatonin seems to promote a Th1-response by increasing IL-12 production. The hormone enhances IL-12 production by cultured monocytes under suboptimal stimulation in a dose-dependent way. The effect of the hormone increases when PBMCs are incubated with melatonin before monocyte isolation. Enhanced IL-12 production by melatonin can also be shown in cultured human mononuclear cells. 相似文献
64.
Fuenzalida M Illanes J Lemus R Guerrero A Oyarzún A Acuña O Lemus D 《Journal of morphology》1999,242(3):295-309
Using tartrate-resistant acid phosphatase (TRAP), we examined the cytodifferentiation of odontoclast cells in resorbing areas of dental tissues during the replacement of teeth in a polyphyodont lizard, Liolaemus gravenhorsti. We also report, by means of Lectin-HRP histochemistry, the distribution pattern of some specific sugar residues of TRAPase-positive cells. For detection of TRAPase activity, the azo dye-coupling technique was used. Lectin binding sites were demonstrated by means of specific HRP-lectins. The process of tooth resorption was divided into four stages: 1) preresorption-the wall of the dental pulp is covered with an odontoblast layer, and no TRAP-positive cells are in the dental pulp; 2) early resorption-TRAP-positive multinucleate odontoclasts are present on the dental wall, but the rest of the pulp surface is still covered with an odontoblast layer; 3) later resorption-the entire surface of the pulp chamber is lined with multinucleate odontoclasts; and 4) final resorption-the tooth has been totally resorbed. Odontoclasts are usually detached from the resorbed surface, and show signs of degeneration. Of the six lectins used, PNA, ECA, and UEA-1 bind to multinucleated but not mononuclear cells. All the remaining lectins, BS-1, RCA(120), and LTA showed no binding to any cells of the teeth. The significance of saccharidic moieties such as acetyl-galactosamine, acetyl-glucosamine, and fucose sugar residues is difficult to ascertain. Perhaps these oligosaccharides might be borne on molecules associated with odontoclastic resorption or associated with multinucleation of odontoclasts after attachment to the dentine surface. 相似文献
65.
Innate protection conferred by fucosylated oligosaccharides of human milk against diarrhea in breastfed infants 总被引:4,自引:0,他引:4
Newburg DS Ruiz-Palacios GM Altaye M Chaturvedi P Meinzen-Derr J Guerrero Mde L Morrow AL 《Glycobiology》2004,14(3):253-263
To test the hypothesis that human milk fucosyloligosaccharides are part of an innate immune system, we addressed whether their expression (1) depends on maternal genotype and (2) protects breastfed infants from pathogens. Thus the relationship between maternal Lewis blood group type and milk oligosaccharide expression and between variable oligosaccharide expression and risk of diarrhea in their infants was studied in a cohort of 93 Mexican breastfeeding mother-infant pairs. Milk of the 67 Le(a-b+) mothers contained more LNF-II (Le(a)) and 3-FL (Le(x)) (oligosaccharides whose fucose is exclusively alpha 1,3- or alpha 1,4-linked) than milk from the 24 Le(a-b-) mothers; milk from Le(a-b-) mothers contained more LNF-I (H-1) and 2'-FL (H-2), whose fucose is exclusively alpha 1,2-linked. The pattern of oligosaccharides varied among milk samples; in each milk sample, the pattern was summarized as a ratio of 2-linked to non-2-linked fucosyloligosaccharides. Milks with the highest ratios were produced primarily by Le(a-b-) mothers; those with the lowest ratios were produced exclusively by Le(a-b+) mothers (p<0.001). Thus maternal genetic polymorphisms expressed as Lewis blood group types are expressed in milk as varied fucosyloligosaccharide ratios. The four infants who developed diarrhea associated with stable toxin of Escherichia coli were consuming milk with lower ratios (4.4 +/- 0.8 [SE]) than the remaining infants (8.5 +/- 0.8; p<0.001). Furthermore, the 27 infants who developed moderate to severe diarrhea of any cause were consuming milk with lower ratios (6.1 +/- 0.9) than the 26 who remained healthy (10.5 +/- 1.9; p = 0.042). Thus, milk with higher 2-linked to non-2-linked fucosyloligosaccharide ratios affords greater protection against infant diarrhea. We conclude that specific oligosaccharides constitute a major element of an innate immune system of human milk. 相似文献
66.
Sabariego Ruiz S Díaz de la Guardia Guerrero C Alba Sánchez F 《Revista iberoamericana de micología》2004,21(3):121-127
This study analysed the seasonal and intradiurnal behaviour of fungal spores from Alternaria and Cladosporium on air samples collected in the city of Almeria (SE Spain), as well as the influence that meteorological parameters have on the concentration of these type of spores. Aerobiological sampling was made during four years (1998-2001) using a Hirst-type volumetric spore trap. Spores of Alternaria and Cladosporium were detected throughout the year, reaching the highest concentrations from May to October. The diurnal patterns of these taxons reflected a presence similar of spores during a 24 h period, with values horary close to 4% of total sampling daily. The correlations show a positive association with temperature, hours of sunshine and accumulate rainfall, but negative with daily rainfall. 相似文献
67.
Age at acquisition of Helicobacter pylori infection: comparison of two areas with contrasting risk of gastric cancer 总被引:2,自引:0,他引:2
Background. Helicobacter pylori infection is usually acquired during childhood and is a known risk factor for the development of gastric malignancies in adulthood. It has been reported that early age at first infection may determine a neoplastic outcome in adults. The purpose of this study was to determine the prevalence of Helicobacter pylori infection in children residing in areas with high (Pasto) and low risk (Tumaco) of gastric cancer in Colombia to evaluate whether differences in the age of acquisition of H. pylori infection were present in the two populations. Materials and Methods. The study sample was based on a census taken in 1999. Using the 13C‐urea breath test, we compared the prevalence of H. pylori infection among children aged 1–6 years. Results. Among 345 children in Pasto, 206 (59.7%) were H. pylori‐positive, compared with 188 (58.6%) among 321 children in Tumaco. The two populations share a common pattern of very early age at infection and marked increase in prevalence during the first 4 years of life. No differences in any one year were observed when comparing the two groups. Conclusions. The prevalence of infection was similarly high and increased with age in both populations. In these populations the age of acquisition of H. pylori after 1 year of age does not appear to be a primary factor responsible for the differences in the rates of gastric cancer incidence in adults. Previous findings in adults showed lower prevalence of the most virulent genotypes in Tumaco compared to Pasto, and bacterial virulence may play a key role in determining cancer outcome. 相似文献
68.
Saks V Kuznetsov A Andrienko T Usson Y Appaix F Guerrero K Kaambre T Sikk P Lemba M Vendelin M 《Biophysical journal》2003,84(5):3436-3456
Heterogeneity of ADP diffusion and regulation of respiration were studied in permeabilized cardiomyocytes and cardiac fibers in situ and in silico. Regular arrangement of mitochondria in cells was altered by short-time treatment with trypsin and visualized by confocal microscopy. Manipulation of matrix volumes by changing K(+) and sucrose concentrations did not affect the affinity for ADP either in isolated heart mitochondria or in skinned fibers. Pyruvate kinase (PK)-phosphoenolpyruvate (PEP) were used to trap ADP generated in Ca,MgATPase reactions. Inhibition of respiration by PK-PEP increased 2-3 times after disorganization of regular mitochondrial arrangement in cells. ADP produced locally in the mitochondrial creatine kinase reaction was not accessible to PK-PEP in intact permeabilized fibers, but some part of it was released from mitochondria after short proteolysis due to increased permeability of outer mitochondrial membrane. In in silico studies we show by mathematical modeling that these results can be explained by heterogeneity of ADP diffusion due to its restrictions at the outer mitochondrial membrane and in close areas, which is changed after proteolysis. Localized restrictions and heterogeneity of ADP diffusion demonstrate the importance of mitochondrial functional complexes with sarcoplasmic reticulum and myofibrillar structures and creatine kinase in regulation of oxidative phosphorylation. 相似文献
69.
Shirai K Guerrero I Huerta S Saucedo G Castillo A Obdulia Gonzalez R Hall GM 《Enzyme and microbial technology》2001,28(4-5):446-452
Fermentation conditions and microorganisms were determined, based on acid production, glucose concentration as carbohydrate source. Inoculation levels to obtain a stable shrimp waste silage were also determined. Shrimp waste ensilation was an efficient method of preservation, allowing the recovery of chitin and another added-value products such as pigments, proteins and enzymes. From the various lactic acid bacteria tested, Lactobacillus pentosus and Lactobacillus sp. (B2) were the best lactic acid producers, although small quantities of acetic acid were detected in samples inoculated with Lactobacillus pentosus. Therefore B2 was chosen for the analysis of glucose consumption as well as for the determination of optimum inoculation levels. The best results were obtained at 10% (w/w wet basis) and 5% (v/w wet basis) respectively. Presence of starters and initial glucose concentration were critical factors in the fermentation of shrimp waste. High initial glucose and starter concentrations reduced the time and increased the amount of lactic acid produced. The fermentation pattern changed during ensilation from hetero to homofermentative. Shrimp waste ensilation prevented the growth of spoilage microorganisms keeping their microbial counts steady and pH values within the acid region. 相似文献
70.
Systematic Parasitology - Four new species of Molinema (Filarioidea: Onchocercidae), parasites of echimyid rodents in South America, are described: M. algardneri n. sp. from Proechimys... 相似文献